Fototerapinin Hücre Kinetiği Üzerine Etkisi

Abstract

Sağlıklı yenidoğanlardan doğumu izleyen ilk bir hafta içinde ve hiperbilirübinemik yenidoğanlardan fototerapi öncesi, sonrası ve iki gün sonrası alınan periferik kan, 10 mg/ml final konsantrasyonda BrdU içeren vasatlara ekilmiştir. Karanlıkta yürütülen kültür işlemi sonunda hazırlanan preparatlarda birinci, ikinci ve üçüncü mitozlar sayılmıştır. Hücre kinetik indeksi (CKI) CKI = (M1 +2.M2+3.M3/100 formülü kullamlarak hesaplanmistir, Elde edilen sonuçlar fototerapinin CKI tzerinde ¢ok belirgin bir etkisinin olmadığını düşündürmüştür.

Keywords

• yenidoğan
• kültür
• Hücre

Effects of Phototherapy on Cell Kinetics

Abstract

Blood cultures were obtained from healthy newborns at first week of life, newborns with hyperbilirubinemia before and after the phototherapy for the analysis of cell kinetics with a repeat two days later. Cultures were carried on dark with a final concentration of BrdU 10 mg/ml. Slides were screened for the first, second and the third mitotic division. Cell Kinetics Index (CKI) was evaluated with the formula of CKI — (M1+2M2+3.M3)/100. Findings were suggested no obvious effect has phototherapy on CKI

Keywords

• suggested
• Cultures
• concentration

References

1. 1. Das BC Sharma T : The fate of X-ray-induced chromosome aberrations in blood lymphocyte culture, Mutat. Res., 176 : 83-104, 1987.
2. 2. Balakrishna Murthy P Abdul Rahiman M Tulpule PG :UV-Induced Chromosome Aberrations, Sister-Chromatid Exchanges and Cell Survival in Cultured Lymphocytes from Malnourished Children. Pediatr. Res., 16 : 663-664, 1982.
3. 3. Vogel F Motulsky AG : Human Genetics, Problems and Approaches. Second ed. : Springer-Verlag Berlin Heidelberg New York, pp 330-359, 1982.
4. 4. Kilbey BJ Legator M Nichols W Ramel C : Handbook of Mutagenicity Test Procedures : Elseiver Scintific Publishing Company, 1877.
5. 5. Tucker JD Christensen ML : Effects of antikoagulants upon sister-chromatid exchanges, cell-cycle kinetics, and mitotic index in human peripheral lymphocytes. Mutat. Res., 180 : 225-228, 1987.
6. 6. Mutchinick O Ruz L Gonsebatt ME Mauleon P Lisker R Garcia G : Frequency and Types of Induced Spontaneous Chromosome Aberrations in Relation to Cell Kinetics, Hum. Genet., 59 : 137-140.
7. 7. Korenberg IR Freedlender EF : Giemsa tecnique for the detection of SCE. Chromosoma, 48 : 355-360, 1874.
8. 8.Demirsoy D Tungbilek E Oran O : Fototerapinin kromozomlar uzerindeki etkisinin in vivo

9. 9.Sideris EG Papageorgiou GC Charalampous SC Vitsa EM : A Spectrum Response Study on Single Strand DNA Breaks Sister Chromatid Exchanges. and Lethality Induced by Phototherapy Lights. Pediatr. Res., 15 : 1019-1023, 1981
10. 10.Hatcher NH Risemberg HM Powers MM Hook EB : Sister Chromatid Exchange and Phototherapy. Mutat. Res., 60 : 401, 1970.