The evaluation of the oxidative stress index of reproductive tissues and serum thiol/disulphide homeostasis during estrous cycle in bitches

Yıl 2020, Cilt: 67 Sayı: 1, 87 - 94, 26.12.2019

Duygu Baki Acar Mürşide Ayşe Demirel Saadet Özen Dizakar Akarca Muhammed Kürşad Birdane

https://doi.org/10.33988/auvfd.604496

Öz

The aim of this study was firstly to determine
the relationship between estrous cycle and oxidative stress in reproductive
tissues in bitches. This research was performed in twenty-nine healthy bitches
from different breeds and of varied ages (the range was 2-5 years) that were
brought to the clinic for routine ovariohysterectomy. The stages of estrous
cycle were detected using by vaginal cytology, blood progesterone level and
histological findings. Ovarian, oviduct and uterine tissues were taken into Eppendorf
tubes for oxidative stress index and stored at -80°C until analyses. Also, another
part of these tissues were fixed in 10% formalin solution. There were no
significant differences (P > 0.05) among total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) values in the reproductive tissues in
concerning estrous cycle stages. However, there were remarkable correlations
between oxidative stress parameters and reproductive tissues in different
estrus stages in bitches. In
conclusion, the physiological values of TAS and TOS concentration, and OSI in
the ovarian, oviduct and uterine tissues during estrous cycle were firstly
defined in this article. The serum thiol/disulphide homeostasis was also
determined in the estrous cycle of bitches. We have found that there are
remarkable variations of oxidative stress balance in the ovary, oviduct and
uterus concurrently, during estrous cycle in the bitches. Besides, significant
correlations between oxidative stress parameters and estrous cycle stages in
the reproductive tissues were observed in the present study.    

Anahtar Kelimeler

Bitch, estrous, oxidative stress index, progesterone, thiol/disulphide

Kaynakça

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