DEVELOPMENT OF CLCUV TOLERANT UPLAND CULTIVARS THROUGH INTROGRESSIO

Öz

Cotton Leaf Curl Virus (CLCuV) is a major threat to global cotton production, causing significant yield losses, particularly in Pakistan. Upland cotton (Gossypium hirsutum) lacks natural resistance to CLCuV, and the only viable solution is the introgression of resistance from wild species, such as Gossypium arboreum and Gossypium anomalum, which have shown resistance or immunity to the virus. In this study, interspecific hybridization techniques were employed to transfer CLCuV resistance from these diploid species into tetraploid G. hirsutum. Hybridization, polyploidy induction using colchicine, and backcrossing were applied to develop virus-resistant cotton varieties. The developed hybrids were screened for CLCuV resistance in greenhouse and field conditions. Several hybrid combinations exhibited varying degrees of resistance, with the highest recorded resistance of 33.0% in a multispecies hybrid combination. Additionally, plants with good fiber quality and high seed cotton yield were identified. Virus-resistant plants, including those with extra-long fiber length, were evaluated for their potential in breeding programs. The CIM-608 variety, approved in 2013, and the Cyto-124 variety, approved in 2016, demonstrate promising characteristics such as high seed cotton yield, good fiber quality, and resistance to pests, making them valuable for enhancing cotton production and food security. These results highlight the success of introgressing CLCuV resistance into upland cotton and provide a foundation for future breeding programs aimed at improving cotton's resistance to viral diseases and enhancing its economic traits

Anahtar Kelimeler

• Cotton
• Cotton Leaf Curl Virus (CLCuV)
• Hybridization
• polyploidy
• gossypium spp

DEVELOPMENT OF CLCUV TOLERANT UPLAND CULTIVARS THROUGH INTROGRESSION

Öz

Cotton Leaf Curl Virus (CLCuV) is a major threat to global cotton production, causing significant yield losses, particularly in Pakistan. Upland cotton (Gossypium hirsutum) lacks natural resistance to CLCuV, and the only viable solution is the introgression of resistance from wild species, such as Gossypium arboreum and Gossypium anomalum, which have shown resistance or immunity to the virus. In this study, interspecific hybridization techniques were employed to transfer CLCuV resistance from these diploid species into tetraploid G. hirsutum. Hybridization, polyploidy induction using colchicine, and backcrossing were applied to develop virus-resistant cotton varieties. The developed hybrids were screened for CLCuV resistance in greenhouse and field conditions. Several hybrid combinations exhibited varying degrees of resistance, with the highest recorded resistance of 33.0% in a multispecies hybrid combination. Additionally, plants with good fiber quality and high seed cotton yield were identified. Virus-resistant plants, including those with extra-long fiber length, were evaluated for their potential in breeding programs. The CIM-608 variety, approved in 2013, and the Cyto-124 variety, approved in 2016, demonstrate promising characteristics such as high seed cotton yield, good fiber quality, and resistance to pests, making them valuable for enhancing cotton production and food security. These results highlight the success of introgressing CLCuV resistance into upland cotton and provide a foundation for future breeding programs aimed at improving cotton's resistance to viral diseases and enhancing its economic traits

Anahtar Kelimeler

• Cotton
• Cotton Leaf Curl Virus (CLCuV)
• Hybridization
• polyploidy
• gossypium spp

Kaynakça

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