EFFECTS OF ROYAL JELLY ON SEX AND GROWTH HORMONES OF MALE AND FEMALE RATS

Öz

The royal jelly consumed by the queen bee throughout her life causes her reproductive functions to differ. The potential impact of royal jelly consumption on hormonal balance during adolescence is an important issue. 128 female and male Wistar albino rats were used in the study. 400 mg/kg royal jelly was administered by gavage to separate male and female groups for 5, 6, 7, and 8 weeks. At the end of each week, blood samples were taken from rats and the levels of growth hormone, insulin-like growth factor (IGF-I), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and testosterone (T) sex hormones were determined by radioimmunoassay.Histopathological examination was performed on ovarian and testicular tissues taken from the rats. While the levels of growth hormone IGF-I and the sex hormones FSH and LH did not differ significantly between the groups, the difference between testosterone and estradiol levels was found to be statistically significant. Histopathological examinations revealed edema between the seminiferous tubules of the male experimental groups at week 8. Based on the study results, the controlled use of royal jelly during adolescence was recommended.

Anahtar Kelimeler

• royal jelly
• growth hormone
• testosteron
• estradiol
• adolescence

ARI SÜTÜNÜN DİŞİ VE ERKEK RATLARIN EŞEY VE BÜYÜME HORMONLARI ÜZERİNE ETKİSİ

Öz

Kraliçe arının yaşamı boyunca tükettiği arı sütü onun üreme fonksiyonları açısından farklılaşmasına neden olmaktadır. Ergenlik döneminde arı sütü tüketiminin hormonal dengeyi etkileyebileceği önemli bir konudur. Araştırmada 128 adet dişi ve erkek Wistar albino rat kullanılmıştır. Dişi ve erkek gurupları ayrı olmak üzere 5,6,7 ve 8 haftalık deney guruplarındaki ratlara 400 mg/kg arı sütü gavajla verilmiştir. Her hafta sonunda ratlardan alınan kan örneklerinde büyüme hormonu insülin benzeri büyüme faktörü (IGF-I) ile folikül uyarıcı hormon (FSH), luteinizan hormon (LH), östradiol (E2) ve testosteron (T) eşey hormonu seviyeleri radyoimmnuassay ile tespit edilmiştir. Ratlardan alınan ovaryum ve testis dokularında histopatolojik inceleme yapılmıştır. Çalışma sonunda büyüme hormonu IGF-I ile eşey hormonları FSH ve LH seviyeleri guruplar arasında önemli oranda değişmezken testosteron ve östradiol hormon seviyeleri arasındaki fark istatistiki olarak önemli bulunmuştur. Histopatolojik incelemeler neticesinde 8.haftada erkek deney gruplarına ait seminifer tübüllerin arasında ödem olduğu tespit edilmiştir. Araştırma sonuçlarına dayanılarak ergenlik döneminde arı sütünün kontrol altında kullanılması önerilmiştir.

Anahtar Kelimeler

• arı sütü
• büyüme hormonu
• testosteron
• estradiol
• ergenlik

Kaynakça

1. 1.Silici, S., Ekmekcioglu, O., Kanbur, M., & Deniz, K. (2011). The protective effect of royal jelly against cisplatin-induced renal oxidative stress in rats. World Journal of Urology, 29, 127–132. https://doi.org/10.1007/s00345-010-0543-5
2. 2. Kunugi, H., & Mohammed Ali, A. (2019). Royal jelly and its components promote healthy aging and longevity: from animal models to humans. International Journal of Molecular Science, 2 (20), 4662. https://doi.org/10.3390/ijms20194662
3. 3. Cho, K.H., Nam, H.S., Bahuguna, A., & Kim, J.E. (2024). Long-term supplementation of royal jelly (raydel®) ımproves zebrafish growth, embryo production and survivability, blood lipid profile and functionality of vital organs: a 72-weeks’ consumption study. Pharmaceuticals, 17, 324.
4. 4. Schole, J. (1982). Theory of metabolic regulation including hormonal effects on the molecular level. Journal of Theoretical Biology, 96(4),579-615.
5. 5. Malik Aubead, N. (2021). Role of sex hormones in human body. IntechOpen. doi: 10.5772/intechopen.95778.
6. 6. Das, P.K., Mukherjee, J., & Banerjee, D. (2023). Female Reproductive Physiology. In: Das, P.K., Sejian, V., Mukherjee, J., Banerjee, D. (eds) Textbook of Veterinary Physiology. Springer, Singapore. https://doi.org/10.1007/978-981-19-9410-4_22 7. Andina, I. (2023). An Overview of the Female Reproductive System: A Narrative Literature Review. Sriwijaya Journal of Obstetrics and Gynecology, 1(2), 73-80. https://doi.org/10.59345/sjog.v1i1.25.
7. 8. Frantz, A.G., & Rabkin, M. (1965). Effects of estrogen and sex difference on secretion of human growth hormone. Journal of Clinical Endocrinology andl Metabolism, 25, 1470-80.
8. 9. Parker, M.W., Johanson, A.J., Rogol, A.D., Kaiser, D.L., & Blizzard, R.M. (1984). Effect of testosterone on somatomedin-C concentrations in prepubertal boys. Journal of Clinical Endocrinology and Metabolism, 58, 87-90.

9. 10. Kusec, V., Virdi, A.S., Prince, R., & Triffitt, J.T. (1998). Localization of estrogen receptor-alpha in human and rabbit skeletal tissues. Journal of Clinical Endocrinology and Metabolism, 83, 2421-8.
10. 11. Braidman, I.P., Hainey, L., Batra, G., Selby, P.L., & Saunders, P.T. et al. (2001). Localization of estrogen receptor beta protein expression in adult human bone. Journal of Bone and Mineral Research, 16, 214-20.
11. 12. Al-Eisa, R., & Al-Nahari, H. (2017). The attenuating effect of Royal Jelly on Hormonal Parameters in Aluminum Chloride (AlCl3) intoxicated rats. International Journal of Pharmaceutical Research and Allied Sciences, 6(2), 70–85.
12. 13. Ahmed, M.M., El-Shazly, S.A., Alkafafy, M.E., Mohamed, A.A., & Mousa, A.A. (2018). Protective potential of royal jelly against cadmium-induced infertility in male rats. Andrologia, 50(5), e12996.
13. 14. Yang, A., Zhou, M., Zhang, L., Xie, G., & Chen, H., et al. (2012). Influence of royal jelly on the reproductive function of puberty male rats. Food and Chemical Toxicology, 50(6), 1834–40.
14. 15. Khazaei, F., Ghanbari, E., & Khazaei, M. (2021). Improved hormonal and oxidative changes by Royal Jelly in the rat model of PCOS: an experimental study. International Journal of Reproductive Biomedicine, 19(6),515.
15. 16. Ab Hamid, N., Abu Bakar, A.B., Mat Zain, A.A., Nik Hussain, N.H., & Othman, Z.A. et al. (2020). Composition of royal jelly (rj) and its anti-androgenic effect on reproductive parameters in a polycystic ovarian syndrome (pcos) animal model. Antioxidants, 9(6), 499.
16. 17. Liu, Y., Wu, D., Wang, K., Chen, H., Xu, H. et al. (2022). Dose-dependent effects of royal jelly on estrogen-and progesterone-induced mammary gland hyperplasia in rats. Molecular Nutrition and Food Research, 66(5), 2100355.
17. 18. Husein, M., & Kridli, R. (2002). Reproductive responses following royal jelly treatment administered orally or intramuscularly into progesterone- treated Awassi ewes. Animal Reproduction Science, 74(1–2), 45–53.
18. 19. Mishima, S., Suzuki, K.M., Isohama, Y., Kuratsu, N., & Araki, Y. et al. (2005). Royal jelly has estrogenic effects in vitro and in vivo. Journal of Ethnopharmacology,101(1–3), 215–20.
19. 20. Ghanbari, E., Khazaei, M.R., Khazaei, M., & Nejati, V. (2018). Royal jelly promotes ovarian follicles growth and increases steroid hormones in immature rats. International Journal of Fertility and Sterility, 11 (4), 263-269. https:// doi. org/ 10. 22074/ ijfs. 2018. 5156. 21. Pi̇rgon, Ö., Atar, M., Çi̇riş, M., & Sever, M. (2019). Effects of royal jelly supplementation on growth plate zones and longitudinal growth in young rats. Mellifera, 19 (2), 1-13.
20. 22. Shen, Sr., L., Liu, X., Chen, Y., Shi, F., Lai Sr., C.Q. (2020). Major royal jelly proteins accelerate onset of puberty and promote ovarian follicular development in ımmature female mice. Current Developments in Nutrition, 4.
21. 23. Bulut, S. (2018). Ratlarda Lokal ve Sistemik Arı Sütü ile Birlikte Demineralize Kemik Grefti Uygulamalarının Kalvaryal Kemik Defekti İyileşmesi Üzerine Etkisinin İncelenmesi. Abant İzzet Baysal Üniversitesi Diş Hekimliği Fakültesi, Uzmanlık Tezi, Bolu, 116 s.