Teucrium polium L. subsp. polium ve Teucrium scordium L. subsp. scordioides (Schreb). Arcang taksonlarının kimyasal profili ve antioksidan aktivitesi

Abstract

Lamiaceae familyası geniş bir coğrafi yayılım kapasitesine sahip olmakla beraber kimi yerde endemik olarak bulunan türleri bünyesinde barındırmaktadır. Teucrium türleri, bu familyanın bir üyesi olup, tıbbi özelliklerinden dolayı geleneksel yöntemlerde kullanılabilmektedirler. Bu çalışmada da endemik iki Teucrium türünün uçucu yağı içeriği, bileşenleri ve antioksidan aktivitelerinin belirlenmesi amaçlanmıştır. Clevenger aparatı kullanılarak elde edilen uçucu yağların bileşenleri GC-MS ile belirlenmiştir. Antioksidan tayininde: 2,2-difenil-1- pikrilhidrazil (DPPH) serbest radikal süpürme metodu, toplam fenolik madde miktarının belirlenmesinde ise Folin-Ciocaltaeu yöntemi kullanılmıştır. Analiz sonuçlarına göre T. polium subsp. polium ’ un uçucu yağ oranı %0.80 olarak belirlenmiş, uçucu yağın ana bileşenlerinin ise β-pinene (%27.85), germacrene D (%18.84),α-pinene (%14.73) olduğu tespit edilmiştir. T. scordium subsp. scordioides' türünün ise % 0.32 uçucu yağ içerdiği belirlenmiştir. Uçucu yağın major bileşenlerinin ise β-pinene (%16.83), caryophyllene oxide (%13.06),α-pinene (%10.96)olduğu tespit edilmiştir. T. polium subsp. polium ’ un DPPH antiradikal aktivitesi %16.99±0.16, gallik asit bakımından fenolik madde içeriği ise 11.60±2.45mg/100 mlolduğu tespit edilirken, T. scordium subsp. scordioides’in DPPH antiradikal aktivitesi %17.09±1.95, gallik asit bakımından fenolik madde içeriği ise 10.46±2.32 mg/100 ml olarak tespit edilmiştir.

Keywords

• GC-MS
• Lamiaceae
• Teucrium
• Uçucu yağ
• antioksidan aktivite

Chemical profile and antioxidant activities of Teucrium polium subsp. polium and Teucrium scordium subsp. scordioides

Abstract

The Lamiaceae family has a wide geographical distribution capacity and includes endemic species in some places. Teucrium species are members of this family and can be used in traditional methods due to their medicinal properties. The aim of this study was to determine the essential oil content, constituents and antioxidant activities of two endemic Teucrium species. The components of the essential oils obtained using Clevenger apparatus were determined by GC-MS. Antioxidant determination: 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method and Folin-Ciocaltaeu method was used to determine the total phenolic content. According to the results of the analysis, the essential oil ratio of T. polium subsp. polium was determined as 0.80% and the main components of the essential oil were β-pinene (27.85%), germacrene D (18.84%), α-pinene (14.73%). T. scordium subs. scordioides species was found to contain 0.32% essential oil. The major components of the essential oil were found to be β-pinene (16.83%), caryophyllene oxide (13.06%), α-pinene (10.96%). DPPH antiradical activity of T. polium subsp. polium was 16.99±0.16% and phenolic content in terms of gallic acid was 11.60±2.45 mg/100 ml, while DPPH antiradical activity of T. scordium subs. scordioides had DPPH antiradical activity of 17.09±1.95% and phenolic content in terms of gallic acid was 10.46±2.32 mg/100 ml.

Keywords

• GC-MS
• Lamiaceae
• Teucrium
• essential oil
• antioxidant activity

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