Matricaria recutita L. capitulum eksplantının in vitro rejenerasyon potansiyeli

Abstract

Özet Amaç: Bu çalışmada, tıbbi ve aromatik bir bitki olan Matricaria recutita L.' nin in vitro rejenerasyon potansiyeli araştırılmıştır. Metod: Tarla koşullarında yetiştirilen bitkilerden izole edilen kapitulum eksplantlarının sterilizasyon protokolünü belirlemek amacıyla altı farklı sterilizasyon işlemi test edildi. Rejenerasyon çalışmaları için kapitulum eksplantlarına, 24 farklı konsantrasyon ve kombinasyonda BAP (0,5 -1-2 mg/L) ve NAA (0-0,5-1-2 mg/L); KIN (0,5-1-2 mg/L) ve 2,4- D (0-0,5 mg/L) bitki büyüme düzenleyicileri uygulanmıştır. Bulgular: Sterilizasyon amaçlı yapılan deneylerde en iyi sonuçlar, %70’lik etanolde 3 dakika, %30’luk NaOCl’de 10 dakika ve %1’lik AgNO3’te 20 ile elde edilmiştir. Rejenerasyon çalışmaları sonucunda; en iyi sürgün uzunlukları sırasıyla 0,5 mg/L BAP+0,5 mg/L NAA ve 0,5 mg/L BAP+2 mg/L NAA içeren MS besin ortamlarında 3,02 ve 3,00 cm olarak belirlenirken; en iyi eksplant başına sürgün sürgün sayısı sırasıyla 0,5 mg/L BAP+0,5 mg/L NAA ve 0,5 mg/L BAP+2 mg/L NAA bitki büyüme düzenleyicileri içeren MS besin ortamlarında 23,91 ve 18,29 olarak belirlenmiştir. Sonuç: In vitro rejenerasyon çalışmalarından elde edilen sonuçlara göre uygulanan bitki büyüme düzenleyici kombinasyonları açısından, BAP ve NAA; KIN ve 2,4-D uygulamalarına göre daha başarılı sonuçlar vermiştir.

Keywords

• bitki büyüme düzenleyiciler
• Bitki doku kültürü
• oksin
• sitokinin
• Matricaria recutita L.

In vitro rejeneration potential of Matricaria recutita L. capitulum explant

Abstract

Purpose: In this study, the in vitro regeneration potential of Matricaria recutita L., a medicinal and aromatic plant, was investigated Method: Six different sterilization processes were tested to determine the sterilization protocol of capitulum explants isolated from plants grown under field conditions. For regeneration studies, plant growth regulators BAP (0.5-1-2 mg/L) and NAA (0-0.5-1-2 mg/L); KIN (0.5-1-2 mg/L) and 2.4-D (0-0.5 mg/L) were applied to capitulum explants at 24 different concentrations and combinations. Findings: In the experiments carried out for sterilization purposes, the best results were obtained in 70% ethanol for 3 minutes, in 30% NaOCl for 10 minutes and in 1% AgNO3 for 20 minutes. The best shoot lengths were determined as 3.02 and 3.00 cm in MS nutrient medium containing 0.5 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L BAP+2 mg/L NAA, respectively; the best shoot number per explant was determined as 23.91 and 18.29 in MS nutrient medium containing 0.5 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L BAP+2 mg/L NAA plant growth regulators, respectively. Conclusion: According to the results obtained from in vitro regeneration studies, in terms of the applied plant growth regulator combinations, BAP and NAA gave more successful results than KIN and 2.4-D applications

Keywords

• auxin
• cytokinin
• Matricaria recutita L.
• plant growth regulator
• Plant tissue culture

Ethical Statement

Acknowledgement: The article is a section from the Doctoral Thesis conducted by Dr. Betül Kurtuluş Demirci under the supervision of Assoc. Prof. Dr. Burcu Çetin.

Thanks

Acknowledgement: The article is a section from the Doctoral Thesis conducted by Dr. Betül Kurtuluş Demirci under the supervision of Assoc. Prof. Dr. Burcu Çetin.

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