Onobrychis paucijuga Bornm. (Fabaceae)’nın bazı anatomik ve palinolojik özellikleri

Abstract

Özet Amaç: Bu çalışma, endemik tür Onobrychis paucijuga Bornm. (Fabaceae)’nın bazı anatomik ve palinolojik özelliklerini ortaya koymayı ve bu özelliklerin cins içerisindeki taksonomik önemini değerlendirmeyi amaçlamaktadır. Metod: Kök ve gövde anatomik yapıları ışık mikroskobu kullanılarak incelenmiştir. %70 etanolde muhafaza edilen bitki materyallerinden elde edilen el kesitleri gliserin-jelatin ile preparat haline getirilmiş ve Nikon Eclipse 80i mikroskobunda analiz edilmiştir. Polen morfolojisi Wodehouse ve Erdtman yöntemleri kullanılarak incelenmiştir. Ayrıca, ekzin ornamentasyonu ve yüzey ultrastrüktürünü belirlemek amacıyla taramalı elektron mikroskobu (SEM) kullanılmıştır. Bulgular: Kök, çok katlı periderm, parenkimatik hücrelerden oluşan geniş bir korteks ve sekonder floem ile ksilemi ayıran belirgin bir vasküler kambiyum ile karakterize edilen iyi gelişmiş bir sekonder yapıya sahiptir. Korteks bölgesinde ve floeme yakın alanlarda sklerenkimatik lifler gözlenmiştir. Gövde, kollenkimatik hipodermis, çok katlı laküner kollenkima ve iyi gelişmiş iletim dokularını içeren tipik bir dikotil yapı göstermektedir. Yaşlı gövdelerde öz parenkima hücrelerinin parçalanma eğiliminde olduğu belirlenmiştir. Polen taneleri radyal simetrik, izopolar ve trikolpattır. P/E oranına göre, Wodehouse preparasyonunda prolat, Erdtman preparasyonunda ise subprolat şekil göstermektedir. Ekzin ornamentasyonu retikülat-granülat olup, ekzin ince bir tektuma sahip tektat yapıdadır. SEM gözlemleri, retikülat-granülat ekzin ornamentasyonunu doğrulamış ve yüzey yapılarına ilişkin ayrıntılı bilgiler ortaya koymuştur. Sonuç: O. paucijuga’nın anatomik ve palinolojik özellikleri, Onobrychis cinsinin diğer üyeleri ve Fabaceae familyası için bildirilen genel özelliklerle büyük ölçüde uyumludur. Anatomik ve polen morfolojik karakterlerin birlikte değerlendirilmesi, bu endemik türün tanımlanması ve taksonomik değerlendirilmesi açısından önemli ayırt edici özellikler sunmaktadır.

Keywords

• Fabaceae
• Onobrychis paucijuga
• anatomi
• palinoloji
• polen morfolojisi
• SEM

Some anatomical and palynological characteristics of Onobrychis paucijuga Bornm. (Fabaceae)

Abstract

Abstract Purpose: The present study aims to provide some anatomical and palynological characterization of the endemic species Onobrychis paucijuga Bornm. and to assess its taxonomic significance within the genus. Method: Root and stem anatomical structures were examined using light microscopy. Hand sections obtained from plant materials preserved in 70% ethanol were mounted in glycerin–gelatin and analyzed under a Nikon Eclipse 80i microscope. Pollen morphology was investigated using both Wodehouse and Erdtman methods. In addition, scanning electron microscopy (SEM) was employed to examine exine ornamentation and surface ultrastructure. Findings: The root exhibits a well-developed secondary structure with a multilayered periderm, a wide cortex composed of parenchymatous cells, and a distinct vascular cambium separating the secondary phloem and xylem. Sclerenchymatous fibers occur in the cortical region and near the phloem. The stem shows a typical dicotyledonous structure with a collenchymatous hypodermis, multilayered lacunar collenchyma, and well-developed vascular tissues. In older stems, pith parenchyma cells tend to disintegrate. Pollen grains are radially symmetrical, isopolar, and tricolpate. Based on the P/E ratio, they are prolate in the Wodehouse preparation and subprolate in the Erdtman preparation. The exine ornamentation is reticulate-granulate, and the exine is tectate, with a thin tectum. SEM observations confirmed the reticulate-granulate exine ornamentation and revealed detailed surface structures. Conclusion: The anatomical and palynological features of O. paucijuga are largely consistent with those reported for other members of the genus Onobrychis and the family Fabaceae. The combination of anatomical and pollen morphological characters provides valuable diagnostic traits for the identification and taxonomic evaluation of this endemic species.

Keywords

• Fabaceae
• Onobrychis paucijuga
• anatomy
• palynology
• pollen morphology
• SEM

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