The first study in Turkey about the chromosomes of tongue fish, Pegusa lascaris Risso, 1810 Soleidae, Pleuronectiformes , living in the Black Sea

Yıl 2016, Cilt: 9 Sayı: 3, 1 - 7, 15.12.2016

Serkan Saygun Filiz Saygun

Öz

In this study, It was examined the chromosome structures, numbers and other cytogenetic features of sole Pegusa lascaris Risso, 1810 being one of the flatfish species live in the Black Sea, by conventional banding methods. The Sole specimens were obtained from fishermen along the coast of West and Central Black Sea between Zonguldak and Ordu . Gill and fin epithelia tissues derived by dissecting fish samples which used colchicine 0.05% treatment for six hours were incubated in hypotonic solution 0.4% KCl and then treated with Carnoy’s fixative. As a result of karyological examination, it was determined that the chromosome number of P. lascaris showed 2n= 42 including 6 metacentric, 8 submetacentric, 12 subtelocentric and 16 acrocentric chromosomes NF= 56

Anahtar Kelimeler

Tongue fish, Pegusa lascaris, Pleuronectiformes, flatfishes, karyotype

Karadeniz'de yaşayan dil balığı, Pegusa lascaris Risso, 1810 Soleidae, Pleuronectiformes kromozomları konusunda Türkiye'deki ilk çalışma

Öz

Bu çalışmada; Karadeniz’de yaşayan yassı balık Pleuronectiformes türlerinden dil balığının Pegusa lascaris Risso, 1810 kromozom yapıları, sayıları ve sitogenetiksel özellikleri rutin bantlama yöntemleri kullanılarak incelenmiştir. Araştırmada dil balığı örnekleri Batı ve Orta Karadeniz sahili boyunca Zonguldak ve Ordu illeri arası bazı bölgelerden avcılıkla elde edilmiştir. Altı saat süre ile kolşisin % 0,05 muamelesi uygulanan balık örneklerinden diseksiyonla çıkarılan solungaç ve yüzgeç epitel dokuları % 0,4 KCl solüsyonunda inkübe edilmiştir ve sonrasında Carnoy fiksatifi ile muamele edilmiştir. Yapılan karyolojik incelemeler sonucunda P. lascaris balığının 42 diploit sayıda ve 6 metasentrik, 8 submetasentrik, 12 subtelosentrik ve 16 akrosentrik kromozomdan meydana gelen karyotipi NF=56 olduğu tespit edilmiştir

Anahtar Kelimeler

Dil balığı, Pegusa lascaris, Pleuronectiformes, yassı balıklar, karyotip

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