Phytochemical analysis of Syringa vulgaris: Quantitative analysis of natural compounds by LC-ESI-MS/MS

Öz

The significant biological activity exhibited by medicinal plants is attributed to the presence of bioactive compounds, which makes them suitable for applications in the food and pharmaceutical industries. In the case of Syringa vulgaris flowers, a quantitative analysis of its bioactive compound content was conducted using LC-ESI-MS/MS. Among the identified compounds, hesperidin was found to be the majör component, with a concentration of 0.33 mg/g extract. Additionally, the methanol extract of S. vulgaris flowers contained relatively high concentrations of naringenin (0.197 mg/g extract), chlorogenic acid (0.101 mg/g extract), o-coumaric acid (0.056 mg/g extract), salicylic acid (0.045 mg/g extract), and sinapic acid (0.04 mg/g extract). These findings highlight the presence of these bioactive compounds in S. vulgaris flowers and their potential applications in the food and pharmaceutical sectors.

Anahtar Kelimeler

• Syringa vulgaris
• natural compounds
• quantitative analysis
• LC-MS/MS

Syringa vulgaris’in fitokimyasal analizi: LC-ESI-MS/MS ile biyoaktif bileşiklerin kantitatif analizi

Öz

Tıbbi bitkiler, içeriklerindeki biyoaktif bileşik nedeniyle, gıda ve ilaç sanayinde kullanılmalarını sağlayan önemli biyolojik aktivite gösterirler. Syringa vulgaris çiçeklerinin biyoaktif bileşiklerinin kantitatif analizi, LC-ESI-MS/MS ile belirlendi. Hesperidin (0,33 mg/g ekstrakt) ana ürün olarak belirlendi. Ayrıca S. vulgaris çiçeklerinin metanol ekstraktında naringenin (0,197), klorojenik asit (0,101), o-kumarik asit (0,056), salisilik asit (0,045) ve sinapik asit (0,04) yüksek konsantrasyon olarak belirlenmiştir. Tıbbi bitkiler, içerdikleri biyoaktif bileşikler sayesinde gıda ve ilaç endüstrisinde kullanılmalarını sağlayan önemli biyolojik aktiviteye sahiptirler. S. vulgaris çiçeklerinin biyoaktif bileşik içeriğinin kantitatif analizi LC-ESI-MS/MS yöntemiyle gerçekleştirildi. Bu analiz sonucunda, hesperidin (0,33 mg/g ekstrakt) ana bileşen olarak belirlendi. Ayrıca, S. vulgaris çiçeklerinin metanol ekstraktında naringenin (0,197 3 mg/g ekstrakt), klorojenik asit (0,101 mg/g ekstrakt), o-kumarik asit (0,056 mg/g ekstrakt), salisilik asit (0,045 mg/g ekstrakt) ve sinapik asit (0,04 mg/g ekstrakt) yüksek konsantrasyonlarda tespit edildi. Bu bulgular, S. vulgaris çiçeklerinde bu biyoaktif bileşiklerin bulunduğunu ve gıda ve ilaç sektörlerinde potansiyel uygulamalarının olabileceğini göstermektedir.

Anahtar Kelimeler

• Syringa vulgaris
• doğal ürünler
• kantitatif analiz
• LC-MS/M

Kaynakça

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