Helichrysum plicatum’un uçucu, fenolik ve yağ asidi içeriklerinin kromatografik yöntemlerle belirlenmesi

Öz

Anadolu'da yaygın olarak bulunan Helichrysum plicatum, antidiyabetik, spazmolitik, nefroprotektif ve antimutajenik özellikleriyle bilinir ve geleneksel tıpta yaraları iyileştirmek ve mide, karaciğer, diyabet ve böbrek taşlarını tedavi etmek için kullanılır. H. plicatum'un biyolojik aktivitelerinin içerdiği biyoaktif bileşenlerden kaynaklandığı varsayılmaktadır. Bu çalışmada H. pilicatum'un uçucu yağ maddeleri, fenoller ve yağ asidi içerikleri kromatografik yöntemlerle belirlendi. Üç farklı ekstrakt elde edildi: etanol, etanol-su ve metanol-kloroform. Uçucu yağın GC-MS/MS analizinde karyofilen (%17.83) ve α-pinen (%16.69) yüksek miktarlarda tespit edildi. Ekstraktların yağ asidi içeriğinin analizinde ayrıca palmitik asit metil ester (%14.74-26.88), oleik asit metil ester (%12.23-23.38), laurik asit metil ester (%17.88-19.91), oktakosan (%4.55-17.00) ve stearik asit metil ester (%4.3-14.81) ana bileşenler olarak belirlendi. Ekstraktların fenolik içeriğinde etanol ve etanol-su ekstraktlarında yüksek miktarlarda naringenin ve vanilik asit, metanol-kloroform ekstraktlarında ise resveratrol ve klorojenik asit belirlenmiştir. Biyolojik aktivitesi olduğu belirlenen ve bilinen bu bileşiklere dayanarak geleneksel tıpta ve diğer birçok alanda kullanımları doğrulanmıştır. Bu çalışmanın H. plicatum bitkisinin detaylı fitokimyasal analizi için in vivo ve in vitro çalışmalara ışık tutması beklenmektedir. Bu nedenle gıda, kozmetik ve farmakolojide kullanımı yaygınlaştırılabilir.

Anahtar Kelimeler

• Helichrysum pilicatum
• fenolik
• fitokimyasal analiz
• uçucu yağ
• yağ asidi

Determination of the volatile, phenolic and fatty acid contents of Helichrysum plicatum by chromatographic methods

Öz

Helichrysum plicatum, which is widely distributed in Anatolia, is known for its antidiabetic, spasmolytic, nephroprotective and antimutagenic properties and is used in traditional medicine to heal wounds and treat stomach, liver, diabetes and kidney stones. It is assumed that the biological activities of H. plicatum are due to the bioactive components it contains. In this study, the volatile oil substances, phenols and fatty acid contents of H. pilicatum were determined by chromatographic methods. Three different extracts were obtained: ethanol, ethanol-water and methanol-chloroform. Caryophyllene (17.83%) and α-pinene (16.69%) were detected in high amounts in GC-MS/MS analysis of the essential oil. Palmitic acid methyl ester (14.74-26.88%), oleic acid methyl ester (12.23-23.38%), lauric acid methyl ester (17.88-19.91%), octacosane (4.55-17.00%) and stearic acid methyl ester (4.3-14.81%) were also determined as the main components in the analysis of fatty acid content of the extracts. In the phenolic content of the extracts, naringenin and vanillic acid were determined in high amounts in ethanol and ethanol-water extracts, resveratrol and chlorogenic acid in methanol-chloroform extracts. Based on these compounds determined and known to have biological activity, their use in traditional medicine and many other areas was confirmed. It is expected that this study will shed light on in vivo and in vitro studies for detailed phytochemical analysis of H. plicatum plant. Therefore, its use in food, cosmetics and pharmacology can be widespread.

Anahtar Kelimeler

• fatty acid
• Helichrysum plicatum
• phenolic
• phytochemical analysis
• volatile oil

Kaynakça

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