Phytochemistry of Melilotus albus flowers: Antioxidant activity by sensory, quantitative analysis of bioactive compounds by LC-ESI-MS/MS

Öz

Plants have gained substantial attention in drug development because of their inclusion of bioactive compounds. The bioactive compounds found in plants are known as secondary metabolites, and they play a crucial role in the pharmaceutical industry. Many of the drugs in use today have been developed by drawing inspiration from biologically active natural compounds. Quantification of phenolics in the methanol extract of Melilotus albus flowers was determined by LC-MS/MS, and coumarin was found as the major compound. DPPH antioxidant activity was measured by sensory analysis, and M. albus flowers were found to have moderate antioxidant activity (40.57%). These flowers were also found to contain totoal phenolic of 129.22 mg GA/g of plant extract. Since M. albus flowers contain coumarin as the main product, related plant flowers may be the source of coumarin and an antioxidant agent.

Anahtar Kelimeler

• Antioxidant activity
• Quantitative analysis
• Melilotus albus
• Natural products

Melilotus albus çiçeklerinin fitokimyası: LC-ESI-MS/MS ile biyoaktif bileşiklerin kantitatif analiziyle ve sensor ile antioksidan analizi

Öz

Bitkiler, biyoaktif bileşikler içermeleri nedeniyle ilaç geliştirmede önemli ilgi görmüştür. Bitkilerde bulunan biyoaktif bileşiklere sekonder metabolitler denir ve bunlar ilaç endüstrisinde önemli bir rol oynar. Günümüzde kullanılan ilaçların çoğu biyolojik olarak aktif doğal bileşiklerden ilham alınarak geliştirilmiştir. Fenoliklerin kantitatif analizi LC-MS/MS ile belirlendi ve ana bileşik olarak kumarin bulundu. DPPH antioksidan aktivitesi sensör analiz ile ölçüldü ve M. albus çiçeklerinin iyi orta derecede aktiviteye sahip olduğu belirlendi (40.57). Bu çiçeklerin ayrıca toplam fenolik içeriği 129.22 mg GA/g olarak bulundu. M. albus çiçekleri ana ürün olarak kumarin içerdiğinden, ilgili bitki çiçekleri kumarin ve antioksidan ajanın kaynağı olabilir.

Anahtar Kelimeler

• Antioksidan aktivite
• Kantitatif analiz
• Melilotus albus
• Doğal ürünler

Kaynakça

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