Morphological and molecular characterization of Myzocallis coryli on Corylus avellana in Artvin (Türkiye)

Yıl 2025, Cilt: 8 Sayı: 2, 52 - 63, 31.12.2025

Hayal Akyıldırım Beğen , Didem Tunalı

https://doi.org/10.38059/biodiversity.1841725

Öz

This study aims to identify aphid species observed on hazelnut (Corylus avellana) trees in Artvin and to characterize them at the molecular level. Field surveys conducted in the districts of Borçka, Kemalpaşa, Arhavi, and Hopa revealed that the species Myzocallis coryli was present in all regions at low densities , remaining well below the economic damage threshold. Molecular analyses using COI gene region with Neighbour Joining (NJ) methods demonstrated that the resulting tree topologies were consistent with one another and that species-level distinctions were clearly resolved. Individuals of M. coryli collected from different districts in Türkiye were included in the ML analysis together with reference sequences obtained from the GenBank database. Samples from Hopa, Arhavi, and Kemalpaşa clustered within the same clade as the reference sequences with high bootstrap support (99%), confirming that the specimens belong to the species M. coryli in accordance with morphological identifications. The close proximity of the three populations also indicates low intraspecific genetic variation among Turkish populations. The estimated crown age of Myzocallis is approximately 0.38–0.45 million years ago (Ma), indicating a relatively recent diversification during the Late Pleistocene.

Anahtar Kelimeler

Artvin , Corylus avellana , hazelnut , Myzocallis coryli , Türkiye

Teşekkür

The authors would like to express their sincere gratitude to Damla İpek Topak and Batuhan Yaman Yakın for their dedicated support and valuable contributions to the laboratory work. We also thank the Artvin Çoruh University Science and Technology Application and Research Center (BİLTEKMER), where the laboratory analyses were conducted, and the Artvin Çoruh University Herbarium (ARTH) for their support during this study. In addition, we gratefully acknowledge the Ali Nihat Gökyiğit Botanical Garden and the 2021-TAB-04 “DNA Barcoding of Medicinal and Aromatic Plants” Specialized Project for their scientific and technical support.

Türkiye'nin Artvin bölgesinde Corylus avellana üzerinde yetişen Myzocallis coryli'nin morfolojik ve moleküler karakterizasyonu

Öz

Bu çalışma, Artvin’deki fındık (Corylus avellana) ağaçlarında görülen afit türlerinin belirlenmesi ve moleküler düzeyde karakterizasyonunu amaçlamaktadır. Borçka, Kemalpaşa, Arhavi ve Hopa ilçelerinde yürütülen arazi çalışmalarında Myzocallis coryli türünün tüm bölgelerde düşük yoğunlukta bulunduğu ve ekonomik zarar eşiğinin oldukça altında kaldığı tespit edilmiştir. Neighbour Joining (NJ), Maximum Likelihood (ML) ve Bayesian Inference (BI) analizleri ile COI gen bölgesi kullanılarak yapılan moleküler analizlerde, elde edilen ağaç topolojilerinin birbirleriyle uyumlu olduğu ve türler arasındaki ayrımın net biçimde yapıldığı görülmüştür. Türkiye’nin farklı ilçelerinden toplanan M. coryli bireyleri, GenBank veri tabanındaki referans sekanslarla birlikte ML analizine dâhil edilmiştir. Hopa, Arhavi ve Kemalpaşa örnekleri, referans dizilerle yüksek bootstrap desteği (%99) altında aynı klad içinde kümelenmiş, bu durum örneklerin morfolojik tanımlamalarla uyumlu biçimde M. coryli türüne ait olduğunu doğrulamıştır. Ayrıca üç popülasyonun birbirine yakın konumlanması, Türkiye’de tür içi genetik varyasyonun düşük olduğunu göstermektedir. Myzocallis cinsinin tahmini taç yaşı yaklaşık 0,38–0,45 milyon yıl önce (My) olup, bu durum Geç Pleyistosen sırasında gerçekleşen görece yakın zamanlı bir çeşitlenmeye işaret etmektedir.

Anahtar Kelimeler

Artvin , Corylus avellana , fındık , Türkiye. , Myzocallis coryli

Kaynakça

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