Medicago lupulina gövdesinin kantitatif analizi ve antioksidan aktivitesinin değerlendirilmesi

Öz

Bitkiler, ilaç endüstrisinde önemli rol oynayan zengin biyoaktif ikincil metabolit içerikleri nedeniyle ilaç keşfinde yoğun ilgi görmektedir. Günümüzde kullanılan terapötik ajanların önemli bir kısmı, doğal olarak oluşan aktif bileşiklerden elde edilmektedir. Bu çalışmada, Medicago lupulina gövdesinin fenolik bileşimi LC-MS/MS ile kantitatif olarak belirlenmiş ve ana ürün olarak salisilik asit (78,4 µg/g ekstrakt) tespit edilmiştir. Metanol ekstraktının antioksidan potansiyeli, DPPH ve ABTS testleri kullanılarak değerlendirilmiştir. Ekstrakt, DPPH ve ABTS testlerinde sırasıyla 14,8 µg/mL ve 8,7 µg/mL IC50 değerleriyle kayda değer antioksidan aktivite göstermiştir. M. lupulina saplarında yüksek konsantrasyonlarda bulunan biyoaktif bileşiklerin tanımlanması, bunların izolasyonuna odaklanan çalışmalara önemli katkı sağlayacaktır.

Anahtar Kelimeler

• Kantitatif analiz
• Medicago lupulina gövde
• antioksidan aktivite

Quantitative analysis of Medicago lupulina stem and evaluation of antioxidant activity

Öz

Plants have gained considerable interest in drug discovery owing to their rich content of bioactive secondary metabolites, which play a critical role in the pharmaceutical industry. A significant proportion of currently used therapeutic agents are derived from naturally occurring biologically active compounds. In this study, the phenolic composition of Medicago lupulina stem was quantified by LC–MS/MS, revealing salicylic acid as the predominant phenolic compound (78.4 µg/g extract). The antioxidant potential of the methanolic stem extract was evaluated using the DPPH and ABTS assays. The extract exhibited notable antioxidant activity, with IC50 values of 14.8 µg/mL and 8.7 µg/mL in the DPPH and ABTS assays, respectively. Identifying bioactive compounds in M. lupulina stems at high concentrations will significantly contribute to studies aimed at their isolation.

Anahtar Kelimeler

• Quantitative analysis
• Medicago lupulina stem
• antioxidant activity

Kaynakça

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