Antiproliferative activity of silver nanoparticles synthesized from Salvia aethiopis

Öz

Nanotechnology has emerged as a rapidly developing field of science in recent years. It is used in fields such as agriculture, pharmaceuticals, electronics, and food. Plants exhibit broad-spectrum bioactivity due to their bioactive compound contents and are widely and effectively used in the pharmaceutical and food industries. In this study, the antiproliferative activity of the extract and silver nanoparticles (AgNPs) was evaluated using the human colorectal adenocarcinoma cell line (Caco-2), the human pancreatic adenocarcinoma cell line (Capan-1), and the mouse fibroblast cell line (L929) via MTT assay. In Caco-2 cell lines, cell viabilities were reported to be 27.5±0.7% and 36.8±1.7% after treatment with nanoparticles and the extract, respectively, at a 1.0 µg/mL concentration. In Capan-1 cell lines, the viabilities were determined to be 30.3±1.1% and 46.3±0.5% after treatment with AgNPs and the extract at 1.0 µg/mL, respectively.

Anahtar Kelimeler

• Silver nanoparticles
• antiproliferative effect
• natural products.

Salvia aethiopis’den sentezlenen gümüş nanopartiküllerinin antiproliferatif aktivitesi

Öz

Nanoteknoloji, son yıllarda hızla gelişen bir bilim dalı olarak ortaya çıkmıştır. Tarım, ilaç, elektronik ve gıda gibi alanlarda kullanılmaktadır. Bitkiler, içerdikleri biyoaktif bileşikler sayesinde geniş spektrumlu biyoaktivite sergilemekte, ilaç ve gıda endüstrilerinde yaygın ve etkili bir şekilde kullanılmaktadır. Bu çalışmada, MTT testi yoluyla insan kolorektal adenokarsinom hücre hattı (Caco-2), insan pankreatik adenokarsinom hücre hattı (Capan-1) ve fare fibroblast hücre hattı (L929) kullanılarak ekstraktın ve gümüş nanopartiküllerinin (AgNPs) antiproliferatif aktivitesi incelendi. AgNPs yüksek antiproliferatif aktivite sergilediği belirlendi. Caco-2 hücre hatlarında, 1.0 µg/mL konsantrasyonda nanopartiküller ve özüt ile etkileşen hücrelerin, hücre canlılıklarının sırasıyla %27.5±0.7 ve %36.8±1.7 olduğu bildirilmiştir. Capan-1 hücre hatlarında ise, 1.0 µg/mL konsantrasyonda AgNP'ler ve ekstrakt ile etkileşen hücrelerin canlılık oranlarının sırasıyla %30.3±1.1 ve %46.3±0.5 olduğu belirlenmiştir.

Anahtar Kelimeler

• Gümüş nanopartikül
• antiproliferatif etki
• doğal ürünler.

Kaynakça

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