The effects of <em>Azadirachta indica </em>A. Juss. extracts on development on larvae of Colorado Potato Beetle [<em>Leptinotarsa decemlineata</em> Say (Col.:Chrysomelidae)]

Abstract

The effects of Neem Azal T/S and Neemix preparations obtained from Azadirachta indica A. Juss. were investigated on development larvae of Leptinotarsa decemlineata Say.
A. indica preparations were tested to be alternative to chemical insecticide to control L. decemlineata. Bioassays to determine the effect of varying concentrations of extracts were used in three different methods. Third instar larvae of about 30-35 mg weight were used for leaf dipping, larvae dipping and topical application. For all experiments 2.2 cm in diameter leaf disks obtained from potatoes grown in the glasshouse were used. For three bioassays four different concentrations were used, for Neem Azal T/S 15, 25, 50, 100 ppm and Neemix 10, 15, 30, 60 ppm. All of experiments were repeated six times.
The results were determined at larvae stage that duration of larvae and pupae periods have been prolonged for all the methods, high mortality at larvae and pupae stages have been obtained, malformed individuals came out, number of individuals from pupae were decreased and the number of eggs laid by the healthy females of the treated pupae decreased.

Keywords

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<em>Azadirachta indica</em> A. Juss ekstraktlarının Patates böceği [<em>Leptinotarsa decemlineata</em> Say (Col.: Chrysomelidae)]

Öz

p>Azadirachta indica A. Jussbitkisindenelde edilmiş Neem Azal T/S ve Neemix preparatlarının Patates böceği (Leptinotarsa decemlineata Say) larvalarının gelişimine etkileri araştırılmıştır.
A indica preparatlarının etkisini belirlemek için üç farklı yöntem kullanılmıştır. Yaprak daldırma, bireyi daldırma ve topikal aplikasyon yöntemleri 30-35 mg ağırlığındaki üçüncü dönem larvalara uygulanmıştır. Bütün denemelerde 2.2cm çapında serada yetiştirilen patates yapraklarından kesilen diskler kullanılmıştır. Her üç yöntem için, Neem Azal T/S,15, 25, 50, 100 ppm dozlarında ve Neemix 10, 15, 30 ve 60 ppm dozlarında olmak üzere dört farklı dozda kullanılmıştır. Bütün denemeler 6 tekerrürlü olarak yapılmıştır.
Araştırma sonucunda, larva döneminde uygulanan tüm yöntemlerde preparatların doz artışına bağlı olarak, larva ve pupa dönemi süresini uzattığı, larva ve pupa dönemlerinde yüksek oranda ölüme neden olduğu, anormal görünümlü bireylerin meydana geldiği, pupadan çıkan ergin sayısının azaldığı ve pupadan çıkan sağlıklı dişilerin daha az yumurta bıraktığı belirlenmiştir.

Anahtar Kelimeler

• Leptinotarsa decemlineata; Larva; Pupa; Gelişime etki; Fekundite; Neem Azal T/S; Neemix; larvae; pupa; growth effect; fecundity; Neem Azal T/S; Neemix

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