Yıl 2019, Cilt 59 , Sayı 3, Sayfalar 59 - 69 2019-09-30

Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola
Fasulye bitkisinden elde edilen endofit bakterilerin bitki gelişimini teşvik edici ve antagonistik özelliklerinin Pseudomonas syringae pv. phaseolicola etmenine karşı karekterizasyonu

Kamil DUMAN [1] , Soner SOYLU [2]


Pseudomonas syringae pv. phaseolicola (Psp) is a seed-borne bacterium which causes halo blight disease in common bean. This study aimed the determination of plant growth-promoting traits (such as IAA, phosphate solubilization) and antagonistic potentials (such as siderophore and ammonia productions) of endophytic plant growth-promoting bacteria (PGPB) from healthy bean plants growing in different regions against Psp in vitro conditions. A total of 131 bacteria were primarily characterized as putative PGPB and tested for antagonist activity against Psp in dual culture tests. Seventy-one bacterial isolates demonstrated antagonistic activities against Psp isolate in varying ratios. Among these 71 isolates, 30 representative bacterial isolates from the different regions/fields were selected. On the basis of morphological, physiological, biochemical characteristics and confirmation by MALDI-TOF analyses, 30 endophytic antagonist isolates were identified as belonging to 10 genera, 24 different species. According to results obtained, 10 isolates belong to Bacillus spp., 6 isolates belong to Pseudomonas spp., 4 isolates belong to Rhizobium radiobacter, 2 isolates belong to Arthrobacter spp., 2 isolates belong to Achromobacter spanius, 2 isolates belong to Serratia liquefaciens, 1 isolate belongs to Acinetobacter calcoaceticus, Exiguobacterium sp., Microbacterium hydrocarbonoxydans, and Ochrobactrum anthropi. The largest and lowest inhibition zone was produced by endophytic bacterial isolates Pseudomonas gessardii (4.85) and Bacillus licheniformis (1.35). Among the tested antagonist bacterial isolates, 10 isolates were positive for the production of α-amylase, 7 isolates positive for phosphate solubilization, 29 isolates positive for siderophore production, 11 isolates positive for protease production. All selected bacterial isolates produced IAA and ammonia in relatively varying amounts. P. gessardii produced a relatively large amount of extracellular siderophore (5.83), Exiguobacterium sp. produced a relatively large amount of extracellular protease (5.25), P. gessardii and O. anthropi produced a relatively large amount of extracellular IAA (161.39 µg/ml) and Acinetobacter calcoaceticus produced a relatively large amount of phosphatase (2.63). This is the first study reporting bean plants harbor endophytes having plant growth promoting activities with antagonistic potential against Psp.



Pseudomonas syringae pv. phaseolicola (Psp) fasulye bitkisinde Hale yanıklığı hastalığına sebep olan tohum kökenli bir bakteriyel hastalık etmenidir. Bu çalışmanın amacı, farklı bölgelerde yetişen sağlıklı fasulye bitkilerinden izole edilen endofitik bitki gelişimini teşvik eden bakterilerin (PGPB) Psp üzerine antagonistik (siderofor ve amonyak üretimi gibi) ve bitki gelişimini teşvik eden mekanizmalarının (IAA üretimi, fosfatı indirgeme özelliği gibi) in vitro koşullarda karakterizasyonudur. Araştırma sonunda elde edilen 131 PGPB arasında 71 izolat Psp etmenine karşı değişen oranlarda engelleme zonu oluşturmuş olup, bu izolatları temsilen 30 izolatın teşhisleri, antagonistik ve bitki gelişimini teşvik eden mekanizmaları araştırılmıştır. Morfolojik, fizyolojik, biyokimyasal ve MALDI-TOF analizler, elde edilen izolatların 10 cins 24 farklı türe ait olduğunu göstermiştir. Bu izolatlar arasında 10 izolat Bacillus spp., 6 izolat Pseudomonas spp., 4 izolat Rhizobium radiobacter, 2 izolat Arthrobacter spp., 2 izolat Achromobacter spanius, 2 izolat Serratia liquefaciens, 1’er izolat Acinetobacter calcoaceticus, Exiguobacterium sp., Microbacterium hydrocarbonoxydans ve Ochrobactrum anthropi olarak teşhis edilmiştir. Psp’ye karşı en yüksek engelleme zonu Pseudomonas gessardii (4.85) izolatı tarafından, en düşük engelleme zonu ise Bacillus licheniformis (1.35) tarafından oluşturulmuştur. Test edilen izolatlardan 10 izolat α-amylase pozitif, 7 izolat fosfataz pozitif, 29 izolat siderofor üretmiş, 11 izolat proteaz pozitif bulunmuştur. Tüm izolatlar IAA ve amonyak üretimi açısından değişken değerlerde pozitif bulunmuştur. En yüksek siderofor üretimi P. gessardii (5.83 indeks değeri), en yüksek protease üretimi Exiguobacterium sp. (5.25 indeks değeri), en yüksek IAA üretimi P. gessardii ve O. anthropi izolatları (161.39 µg/ml), en yüksek fosfor çözme kapasitesi Acinetobacter calcoaceticus izolatı (2.63 indeks değeri) tarafından gösterilmiştir. Bu çalışma ile ilk kez fasulye bitkisinde bitki gelişimini teşvik eden ve Psp’ye antagonistik etkiye sahip endofit bakteriler elde edilmiştir.


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Birincil Dil en
Konular Fen
Bölüm Makaleler
Yazarlar

Yazar: Kamil DUMAN (Sorumlu Yazar)
Kurum: ANKARA ZİRAİ MÜCADELE MERKEZ ARAŞTIRMA ENSTİTÜSÜ MÜDÜRLÜĞÜ
Ülke: Turkey


Yazar: Soner SOYLU
Kurum: MUSTAFA KEMAL ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ
Ülke: Turkey


Destekleyen Kurum Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Mustafa Kemal Üniversitesi Fen Bilimleri Enstitüsü
Proje Numarası TAGEM-BS-15/09-03/02-07
Teşekkür This study was supported by TAGEM under the project name TAGEM-BS-15/09-03/02-07.
Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { bitkorb597214, journal = {Bitki Koruma Bülteni}, issn = {0406-3597}, eissn = {1308-8122}, address = {}, publisher = {Zirai Mücadele Merkez Araştırma Enstitüsü}, year = {2019}, volume = {59}, pages = {59 - 69}, doi = {10.16955/bitkorb.597214}, title = {Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola}, key = {cite}, author = {DUMAN, Kamil and SOYLU, Soner} }
APA DUMAN, K , SOYLU, S . (2019). Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola. Bitki Koruma Bülteni , 59 (3) , 59-69 . DOI: 10.16955/bitkorb.597214
MLA DUMAN, K , SOYLU, S . "Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola". Bitki Koruma Bülteni 59 (2019 ): 59-69 <https://dergipark.org.tr/tr/pub/bitkorb/issue/48507/597214>
Chicago DUMAN, K , SOYLU, S . "Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola". Bitki Koruma Bülteni 59 (2019 ): 59-69
RIS TY - JOUR T1 - Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola AU - Kamil DUMAN , Soner SOYLU Y1 - 2019 PY - 2019 N1 - doi: 10.16955/bitkorb.597214 DO - 10.16955/bitkorb.597214 T2 - Bitki Koruma Bülteni JF - Journal JO - JOR SP - 59 EP - 69 VL - 59 IS - 3 SN - 0406-3597-1308-8122 M3 - doi: 10.16955/bitkorb.597214 UR - https://doi.org/10.16955/bitkorb.597214 Y2 - 2019 ER -
EndNote %0 Bitki Koruma Bülteni Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola %A Kamil DUMAN , Soner SOYLU %T Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola %D 2019 %J Bitki Koruma Bülteni %P 0406-3597-1308-8122 %V 59 %N 3 %R doi: 10.16955/bitkorb.597214 %U 10.16955/bitkorb.597214
ISNAD DUMAN, Kamil , SOYLU, Soner . "Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola". Bitki Koruma Bülteni 59 / 3 (Eylül 2019): 59-69 . https://doi.org/10.16955/bitkorb.597214
AMA DUMAN K , SOYLU S . Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola. Bitki Koruma Bülteni. 2019; 59(3): 59-69.
Vancouver DUMAN K , SOYLU S . Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola. Bitki Koruma Bülteni. 2019; 59(3): 69-59.