Bazı Lathyrus L. Türlerinin Yağda Çözünen Vitamin İçerikleri

Abstract

Bu çalışmada, HPLC ile Lathyrus L. türlerinin (Lathyrus sylvestris L., Lathyrus sphaericus Retz.; Lathyrus pratensis L., L. aphaca L. var. modestus P.H., L. aphaca L. var. biflorus Post., L. aphaca var. pseudoaphaca (Boiss.) P.H. Davis, Lathyrus odoratus L., Lathyrus hierosolymitanus Boiss., Lathyrus tauricola P.H. Davis) tohumlarındaki yağda çözünen vitamin içerikleri belirlenmiştir. Bu çalışmadan elde edilen veriler göstermiştir ki çalışılan Lathyrus L. türleri yüksek beta karoten içeriğine sahiptirler (146,5±1,73-375,0±3,35 µg/g). Bu çalışmada çalışılan türler arasında Lathyrus pratensis’in en yüksek gamma-tokoferol içeriğine (158,9±3,65 µg/g) sahip olduğunu L. aphaca var. pseudoaphaca (Boiss.) P.H. Davis’in ise en yüksek D3 vitamini içeriğine (43,2±2,18 µg/g) sahip olduğu bulunmuştur. Bununla birlikte, çalışılan Lathyrus L. türlerinin r-tokoferol, a-tokoferol, a-tokoferol asetat, retinol, retinol asetat, D2 ve K1 vitamin içeriklerinin oldukça düşük olduğu bulunmuştur. 

Keywords

• HPLC,Lathyrus,Yağda Çözünen Vitaminler

The Lipide-Soluble Vitamin Contents Of Some Lathyrus Species

Abstract

In the present study, lipide-soluble vitamin contents in the seeds of the Lathyrus L. taxa (Lathyrus sylvestris L., Lathyrus sphaericus Retz.; Lathyrus pratensis L., L. aphaca L. var. modestus P.H., L. aphaca L. var. biflorus Post., L. aphaca var. pseudoaphaca (Boiss.) P.H. Davis, Lathyrus odoratus L., Lathyrus hierosolymitanus Boiss., Lathyrus tauricola P.H. Davis) were determined by using HPLC. Data obtained from present study showed that studied Lathyrus taxa have high beta-caroten content (146,5±1,73-375,0±3,35 µg/g).  present study found that Lathyrus pratensis  has the higesht gamma-tocopherol content (158,9±3,65 µg/g) whilst  L. aphaca var. pseudoaphaca (Boiss.) P.H. Davis has highest D3 vitamin content (43,2±2,18 µg/g) among studied taxa. However, it was found that the r-tocopherol, a-tocopherol, a-tocopherol acetate, retinol, retinol acetate, D2 and K1 vitamins of studied Lathyrus taxa were lowest.

Keywords

• HPLC,Lathyrus,Lipide-soluble vitamins

References

1. 1. Chavana UD., McKenzie DB., Amarowicz R., Shahidi F. 2003. Phytochemical components of beach pea (Lathyrus maritimus L.). Food Chemistry 81, 61–71.
2. 2. Kalogeropoulos N., Chiou A., Ioannou M., Karathanos VT., Hassapidou M., Andrikopoulos NK. 2010. Nutritional evaluation and bioactive microconstituents (phytosterols, tocopherols, polyphenols, triterpenic acids) in cooked dry legumes usually consumed in the Mediterranean countries. Food Chemistry 121;682–690.
3. 3. Geil PB., Anderson JW. 1994. Nutrition and health implications of dry beans: A review. Journal of the American College of Nutrition, 13, 549–558.
4. 4. Pastor-Cavada E., Juan R., Alaziz M., Vioque J. 2011. Nutritional characteristics of seed proteins in 15 Lathyrus species (Fabaceae) from Southern Spain. LWT - Food Science and Technology 44; 1059-1064.
5. 5. Anderson JW., Major AW. 2002. Pulses and lipaemia, short- and long-term effect: potential in the prevention of cardiovascular disease. British Journal of Nutrition, 88 Supl, 3, S263-S271.
6. 6. Rizkalla SW., Bellisle F., Slama, G. 2002. Health benefits of lowglycaemic index foods, such as pulses, in diabetic patients and healthy individuals.British Journal of Nutrition, 88, S255–S262.
7. 7. Yan ZY, Spencer PS., Li ZX., Liang YM., Wang YF., Wang CY., Li FM. 2006. Lathyrus sativus (grass pea) and its neurotoxin ODAP. Phytochemistry 67; 107–121.
8. 8. Shehadeh AA. 2011. Ecogeographic, Genetic and Taxonomic Studies of the GenusLathyrusL. Degree of Doctor of philosophy University of Birmingham p. 401.

9. 9. Arslan M. 2017. Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.). Legume Research, 40(5); 803-810.
10. 10. Vaz Patto MC., Rubiales D. 2014. Lathyrus diversity: available resources with relevance to crop improvemente L. sativus and L. cicera as case studies. Ann. Bot. 113.
11. 11. Maroua G., Nadia Z., Imen F., Neila TF. 2014. Sonia, M. Molecular characterization of Lathyrus species using chloroplast DNAtrnH-psbA. Biochemical Systematics and Ecology 57; 439-444.
12. 12. Davis PH. 1970. Lathyrus L. In: Flora of Turkey and East Aegean Islands, 3, Edinburgh University Press 328-369.
13. 13. Ertekin SA., Saya O. 1990. A new record for the flora of Turkey. Turk. J. Bot., 15 (1), 75-77.
14. 14. Ertekin SA. 1994. A new record for the flora of Turkey. Turk J Bot 18(1):33–34.
15. 15. Davis PH., Mill RR., Kit T. 1988. Flora of Turkey and East Aegean Islands, 10, Edinburgh University Press, 125-126.
16. 16. Maxted N., Goyder DJ. 1988. A new species of Lathyrus Sect. Lathyrus (Leguminosae) Papilionodeae) from Turkey. Kew Bull., 43 (4), 711- 714.
17. 17. Guner F., Ozhatay N. 2000. Lathyrus L. In: Flora of Turkey and the East Aegean Islands. A. Guner, N. Ozhatay, T. Ekim & K.H.C. Baser (eds.), 11. Edinburgh University Press, 92-94.
18. 18. Akpinar N., Akpinar AM., Türkoğlu Ş. 2001. Total lipid content and fatty acid composition of the seeds of some Vicia L. species. Food Chemistry, 74, 449–453.
19. 19. Tamburino R., Guida V., Pacifico S., Rocco M., Zarelli A., Parente A., Maro AD. 2012. Nutritional values and radical scavenging capacities of grass pea (Lathyrus sativus L.) seeds in Valle Agricola district, Italy. Australian Journal of Crop Science, 6(1):149-156.
20. 20. Mondal MMA., Puteh AB. 2014. Spectrum of variability in seed size and content of Protein and Odap in Grass Pea (Lathyrus sativus L.) Germplasm. Legume Research, 37(5):479-482.
21. 21. Llorent EJ., Ortega-Barrales P., Zengin G., Mocan A., Simirgiotis MJ., Ceylan R., Uysal S., Aktumsek A. 2017. Evaluation of antioxidant potential, enzyme inhibition activity and phenolic.
22. 22. Gupta P., Udupa SM., Gupta D.S., Kumar J., Kumar S. 2018. Population structure analysis and determination of neurotoxin content in a set of grass pea (Lathyrus sativus L.) accessions of Bangladesh origin. The C Rop Journal, 6 435–442.
23. 23. Hara A, Radin NS. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal.Biochem. 90 (1) 420-426.
24. 24. Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P. 2002. High-performance liquid chromatographic determination of a-tocopherol in macroalgae. Journal of Chromatography A 976 (1) 277–284.
25. 25. Yılmaz Ö., Keser S., Tuzcu M., Çetintas B. 2007. Resveratrol (trans-3,4’,5-trihydoxystilbene) decreases lipid peroxidation level and protects antioxidant capacity in sera and erytrocytes of old female Wistar rats induced by the kidney carcinogen potassium bromate. Envir. Toxicol. Pharmacol., 24, 79-85.
26. 26. Frias MJ., Miranda ML., Doblado R., Vidal-Valverde C. 2005. Effect of germination and fermentation on the antioxidant vitamin content and antioxidant capacity of Lupinus albus L. var. multolupa. Food Chemistry, 92, 211–220.
27. 27. Oboh G. 2006. Antioxidant properties of some commonly consumed and under utilized tropical legumes. Eur Food Res Technol., 224, 61–65.
28. 28. Ryan E., Galvin K., O’Connor P., Maguire AR., O’Brien NM. 2007. Phytosterol, Squalene, Tocopherol Content and Fatty Acid Profile of Selected Seeds, Grains, and Legumes. Plant Foods Hum Nutr., 62:85–91.
29. 29. Kushi LH, Folsom AR, Prineas RJ, Mink PJ, Wu Y, Bostick RM. 1996. Dietary antioxidant vitamins and death from coronary heart disease in postmenopausal women. N Engl J Med 330:1029–1035.
30. 30. Knekt P, Reunanen A, Jarvinen R, Seppanen R, Heliovaara M, Aromaa A. 1994. Antioxidant vitamin intake and coronary mortality in a longitudinal population study. Am J Epidemiol 139:1180–1189.
31. 31. Fernandez-Marin B., Milla R., Martin-Robles N., Arc E., Kranner I., Becerril JM., Garcia-Plazaola I. 2014. Side-effects of domestication: cultivated legume seeds contain similar tocopherols and fatty acids but less carotenoids than their wild counterparts. BMC Plant Biology, 14,1599.
32. 32. Wyatt CJ., Pe´rez-Carballido S., Me´ndez RO. 1998. a- and ctocopherol content of selected foods in the mexican diet: Effect of cooking losses. Journal of Agricultural and Food Chemistry, 46, 4657–4661.
33. 33. Sahin A., Emre İ., Yilmaz Ö., Genç H., Karatepe M. 2009. Vitamin and fatty acid contents in seeds of some taxa belonging to genus Lathyrus L. growing in Turkey. Acta Botanica Gallica, 156 (3), 331-339.
34. 34. Chavan UD., Shahidi F., Bal AK., McKenzie DB. 1999. Physico-chemical properties and nutrient composition of beach pea (Lathyrus maritimus L.). Food Chem., 66 (1): 43-50.
35. 35. Korus A., Lisiewska Z., Kmiecik W. 2002. Effect of freezing and canning on the content of selected vitamins and pigments in seeds of two grass pea (Lathyrus sativus L.) cultivars at the not fully mature stage. Nahrung/Food 46 (4): 233–237.
36. 36. Mamatha BS., Sageetha RK., Baskaran V. 2011. Provitamin-A and xanthophyll carotenoids in vegetables and food grains of nutritional and medicinal importance. International Journal of Food Science and Technology 2011,46, 315–323.