Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması

Yıl 2019, , 843 - 851, 30.09.2019

Abdulhamit Battal , Gül Görmez , Musa Türker

https://doi.org/10.17798/bitlisfen.544221

Öz

Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazı
türleri içermektedir. Alcea kurdica
Alef. (Devegülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemi
enfeksiyonlarına ve saç kırana karşı kullanılan tıbbi bir bitkidir. Bu
çalışmanın amacı, devegülü hatmi bitkisi için steril şartlar altında etkili bir
çimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürü
çalışmalarında kullanılabilir hale getirilmesidir. Bu amaç için, steril bir
ajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°C
sıcaklıkta inkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesi
uygulamaları test edilmiştir. %5 ve %7.5 sodyum hipoklorit konsantrasyonlarının
sterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipoklorit
konsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme indeksi sonuçlarına göre, kabuğu çentiklenen tohumlar
hızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir.
Yedinci günde, kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürü
için kullanılabilir duruma gelmiştir. Sekizinci günde, yapraklar oluşmaya
başlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu günde
doku kültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma ile
tıbbi öneme sahip devegülü hatmi için etkili bir çimlendirme yöntemi
geliştirilmiştir.

Anahtar Kelimeler

Çimlenme İndeksi, Hatmi, Alcea

The Efficient Germination Method For Marhmallows With Medicinal Importance: The Nicking on Seed Coat

Öz

The genus Alcea
one of the member of Malvaceae family includes some species having medicinal
importance. Alcea kurdica Alef.
(Devegülü Marshmallow) is a medicinal plant used against tonsils, gastric
ulcers, pneumonia, excretory system infections and pelade. The aim of this
study was to develope an efficient germination method under sterile conditions
and to make the plant suitable for tissue culture studies. For this purpose,
three different concentrations (5%, 7.5% and 10%) of the sodium hypochlorite
which is a sterile agent, 42°C incubation and damaging on the seed coat with
blade were tested. While 5% and 7.5% of sodium hypochlorite concentrations were
not sufficient for sterilization, 10% of sodium hypochlorite concentration was
observed to be sufficient. According to the results of the germination index,
while the seeds nicked on coat were easily germinated, it was observed that
incubation at 42°C did not affect the germination. On the seventh day,
cotyledon, cotyledonary node and hypocotyl tissues were ready to use for tissue
culture. On the eighth day, leaves began to form. Leaf, petiole and shoot tip
explants were available to use in tissue culture on the thirtieth day. As a
result, an efficient germination method for devegülü marshmallow having
medicinal importance was developed with this study.

Anahtar Kelimeler

Germination Index, Marshmallow, Alcea

Kaynakça

• [1] Ya T., Gilbert M.G., Dorr L.J. 2007. Malvaceae in Flora of China, Edited by Wu ZY, Raven PH, Hong DY, Vol.12 (Hippocastanaceae through Theaceae), Science Press, Beijing / Missouri Botanical Garden Press, St. Louis, 265-298.
• [2] Kasumov M.A. 1984. Red dye from the hollyhock and its use in food industry. Doklady-Akademiia nauk Azerbaidzhanskoi SSR, 40 (6): 76-79.
• [3] Facciola S. 1990. Cornucopia: a source book of edible plants, Kampong Publications, 677s. California.
• [4] Roberts M.J. 2000. Edible and medicinal flowers, Spearhead Press, 160s. Claremont.
• [5] Lim T.K. 2014. Alcea rosea in Edible Medicinal and Non Medicinal Plants, Edited by Lim TK., Vol.8, Dordrecht, Springer. 292-299.
• [6] Rakhimov D.A., Atkhamova S.K., Khvan A.M. 2007. Pectinic substances from Alcea rosea flowers. Chemistry of Natural Compounds, 43 (6): 685-686.
• [7] Dudek M., Matławska I., Szkudlarek M., 2006. Phenolic acids in the flowers of Althaea rosea var. nigra. Acta Pol Pharm, 63 (3): 207-211.
• [8] Daulatabad C.D. Jamkhandi AM. 2000. Althaea rosea seed oil-a rich source of ricinoleic acid. Journal of the Oil Technologists' Association of India, 32 (1): 8-9.
• [9] Sherwani M.R.K., Chouhan S., Malik A., Parveen S., Sharma S. 2012. Isolation and characterization of cyclopropenoid fatty acids in Althaea rosea seed oil. Int J Res Phytochem Pharmacol, 2 (1): 52-54.
• [10] Barnaulov O.D., Manicheva O.A., Trukhaleva N.A., Kozhina I.S., Fokina N.E., Salikhov S.A. 1985. Alcea-rosea a source of polysaccharides with anti-ulcer activity. Rastitel’nye Resursy, 21: 329-340.
• [11] Mert T., Fafal T., Öztürk B. 2010. Antimicrobial and Cytotoxic Activities of the Extracts Obtained from the Flowers of Alcea Rosea L.. Hacettepe University Journal of the Faculty of Pharmacy, 30 (1): 17-24.
• [12] Yaglıoglu Sahin A., Eser F., Tekin S., Onal A. 2016. Antiproliferative activities of several plant extracts from Turkey on rat brain tumor and human cervix carcinoma cell lines. Frontiers in Life Science, 9 (1): 69-74.
• [13] Kim M.S., Chathuranga K., Kim H., Lee J.S., Kim C.J. 2018. Anti-influenza properties of herbal extract of Althaea rosea in mice. Korean Journal of Veterinary Research, 58 (3): 153-158.
• [14] Naz R., Anis M. 2012. Acceleration of adventitious shoots by interaction between exogenous hormone and adenine sulphate in Althaea officinalis L. Applied biochemistry and biotechnology, 168 (5): 1239-1255.
• [15] Naz R., Anis M., Alatar A.A. 2017. Embling Production in Althaea officinalis L., Through Somatic Embryogenesis and Their Appraisal via Histological and Scanning Electron Microscopical Studies. Applied biochemistry and biotechnology, 182 (3): 1182-1197.
• [16] Naz R., Anis M., Aref I.M., 2015. Management of cytokinin–auxin interactions for in vitro shoot proliferation of Althaea officinalis L.: a valuable medicinal plant. Rendiconti Lincei, 26 (3): 323-334.
• [17] Bouayed J., Piri K., Rammal H., Dicko A., Desor F., Younos C., Soulimani R. 2007. Comparative evaluation of the antioxidant potential of some Iranian medicinal plants. Food Chemistry, 104 (1): 364-368.
• [18] Mati E., de Boer H. 2011. Ethnobotany and trade of medicinal plants in the Qaysari Market, Kurdish Autonomous Region, Iraq. Journal of Ethnopharmacology, 133 (2): 490-510.
• [19] Qader S.W., Awad H.M. 2014. Evaluation of Antioxidant, Antimicrobial and Cytotoxicity of Alcea kurdica Alef. Jordan Journal of Biological Sciences, 147 (1572): 1-5.
• [20] Baiyeri K.P., Mbah B.N. 2006. Surface sterilization and duration of seed storage influenced emergence and seedling quality of African breadfruit (Treculia africana Decne). African Journal of Biotechnology, 5 (15): 1393-1396.
• [21] Ramakrishna N., Lacey J., Smith J.E. 1991. Effect of surface sterilization, fumigation and gamma irradiation on the microflora and germination of barley seeds. International journal of food microbiology, 13 (1): 47-54.
• [22] Younesikelaki F.S., Ebrahimzadeh M.H., Desfardi M.K., Banala M., Marka R., Nanna R.S. 2016. Optimization of seed surface sterilization method and in vitro seed germination in Althaea officinalis (L.)-an important medicinal herb. Indian J. Sci. Technol, 9: 1-6.
• [23] Kozlowski J., Szczyglewska D., Formanowiczowa H. 1989. Biology of germination of medicinal plants seeds. Pt. 14. Seeds of species from Malvaceae family: marsh mallow (Althea officinalis L.) and mallow (Malva silvestris L.). Herba Polonica, 2 (3): 99-107.
• [24] Shooshtarian S., Salehi H. 2010. Enhancing Alcea aucheri (Boiss.) Alef. seed germination by application of some scarification treatments. Advances in Environmental Biology, 4 (2): 216-219.
• [25] Akin M., Ekin Z., Ozmen S., Kaya M. 2019. Seed Dormancy in Rheum ribes L. Collected from Natural Populations in Turkey. International Journal of Scientific and Technological Research, 5 (2): 183-192.