Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması

Abstract

Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazı türleri içermektedir. Alcea kurdica Alef. (Devegülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemi enfeksiyonlarına ve saç kırana karşı kullanılan tıbbi bir bitkidir. Bu çalışmanın amacı, devegülü hatmi bitkisi için steril şartlar altında etkili bir çimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürü çalışmalarında kullanılabilir hale getirilmesidir. Bu amaç için, steril bir ajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°C sıcaklıkta inkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesi uygulamaları test edilmiştir. %5 ve %7.5 sodyum hipoklorit konsantrasyonlarının sterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipoklorit konsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme indeksi sonuçlarına göre, kabuğu çentiklenen tohumlar hızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir. Yedinci günde, kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürü için kullanılabilir duruma gelmiştir. Sekizinci günde, yapraklar oluşmaya başlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu günde doku kültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma ile tıbbi öneme sahip devegülü hatmi için etkili bir çimlendirme yöntemi geliştirilmiştir.

Keywords

• Çimlenme İndeksi,Hatmi,Alcea

The Efficient Germination Method For Marhmallows With Medicinal Importance: The Nicking on Seed Coat

Abstract

The genus Alcea one of the member of Malvaceae family includes some species having medicinal importance. Alcea kurdica Alef. (Devegülü Marshmallow) is a medicinal plant used against tonsils, gastric ulcers, pneumonia, excretory system infections and pelade. The aim of this study was to develope an efficient germination method under sterile conditions and to make the plant suitable for tissue culture studies. For this purpose, three different concentrations (5%, 7.5% and 10%) of the sodium hypochlorite which is a sterile agent, 42°C incubation and damaging on the seed coat with blade were tested. While 5% and 7.5% of sodium hypochlorite concentrations were not sufficient for sterilization, 10% of sodium hypochlorite concentration was observed to be sufficient. According to the results of the germination index, while the seeds nicked on coat were easily germinated, it was observed that incubation at 42°C did not affect the germination. On the seventh day, cotyledon, cotyledonary node and hypocotyl tissues were ready to use for tissue culture. On the eighth day, leaves began to form. Leaf, petiole and shoot tip explants were available to use in tissue culture on the thirtieth day. As a result, an efficient germination method for devegülü marshmallow having medicinal importance was developed with this study.

Keywords

• Germination Index,Marshmallow,Alcea

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