In vivo Determination of Antioxidant Properties of Heterocyclic 4,5- dihydro-1H-1,2,4-triazole-5-one Derivative in Male Rats (Wistar albino)

Abstract

4,5-Dihydro-1H-1,2,4-triazol-5-one derived heterocyclic compounds have an important place in industry, medicine, pharmacy and many other fields of chemistry. Biological activity properties of these compounds have been examined in vitro, it was determined that some of them show high activity. One of these activities is the antioxidant property of 1,2,4-triazol-5-one derivatives. In the present study, the antioxidant properties of 3-methyl4-(3,4-dihydroxybenzylideneamino)-4,5-dihydro-1H-1,2,4-triazol-5-one (3-MTA) were determined in rats by spectrophotometric method in vivo. For this purpose, animals were divided into 4 groups; control group, dimethyl sulfoxide (DMSO) group, 10 mg/kg and 50 mg/kg including MTA compound groups. As a result of the spectrophotometric analyzes, total antioxidant status (TAS) were increased in the serum treated groups of both 3- MTA compounds (P<0.01) and in the renal tissue treated with only 50 mg / kg 3-MTA compounds (P <0.001). On the other hand, total oxidant status (TOS) of kidney tissue was decreased in 50 mg/kg 3-MTA compound administrated group compared to control group (P <0.05). As a result; according to the obtained data, it was concluded that 3-MTA compound did not show toxic effects in dose and duration of rats and it had positive biological activity according to increase in serum and renal TAS levels and decrease in renal TOS levels.

Keywords

• Triazol
• Schiff bazı
• TAD
• TOD

Heterosiklik 4,5-dihidro-1H-1,2,4-triazol-5-on Türevinin Antioksidan Özelliğinin Erkek Ratlarda (Wistar albino) İn vivo Olarak Belirlenmesi

Abstract

4,5-Dihidro-1H-1,2,4-triazol-5-on türevli heterosiklik bileşikler sanayide, tıpta, eczacılıkta ve kimyanın pek çok alanında önemli yere sahiptir. Bu bileşiklerin bazı biyolojik aktivite özellikleri in vitro olarak incelenmiş olup bazılarının yüksek aktivite gösterdikleri belirlenmiştir. Bu aktivitelerden biri de 1,2,4-triazol-5-on türevlerinin göstermiş olduğu antioksidan özelliğidir. Mevcut araştırmada da, 3-metil-4-(3,4-dihidroksibenzilidenamino)-4,5-dihidro-1H-1,2,4-triazol-5-on (3-MTA) bileşiğinin antioksidan özellik gösterip göstermediği ratlarda in vivo olarak spektrofotometrik yöntemle belirlenmiştir. Bu amaçla hayvanlar; kontrol grubu, DMSO grubu, 10 mg/kg ve 50 mg/kg 3-MTA bileşiği içeren gruplar olacak şekilde 4’e ayrılmıştır. Yapılan spektrofotometrik analizler neticesinde kontrol grubuna kıyasla serumda 10 ve 50 mg/kg dozunda 3-MTA bileşiği uygulanan gruplarda, böbrek dokusunda ise 50 mg/kg dozunda 3-MTA bileşiği uygulanan grupta total antioksidan düzeyleri artış göstermiştir (P<0.01). Bununla birlikte; böbrek dokusu total oksidan seviyesinin ise kontrol grubuna göre 50 mg/kg 3-MTA bileşiği uygulanan grupta azaldığı belirlenmiştir (P<0.01). Sonuç olarak; elde edilen verilere göre, 3-MTA bileşiğinin sıçanlarda uygulanan doz ve sürede toksik etki göstermediği, serum ve böbrek TAD düzeyindeki artış ve böbrek TOD düzeyindeki azalmaya göre de olumlu biyolojik aktiviteye sahip olduğu kanısına varılmıştır. 

Keywords

• Triazole
• Schiff base
• TAS
• TOS

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