Molecular Typing with BOX-PCR of Staphylococcus aureus Isolated from Milk Samples

Abstract

Bu çalışmanın amacı Konya bölgesindeki sığır subklinik mastitinden izole edilen 98 Staphylococcus aureus'u REP ve BOX primerleri ile karakterize etmektir. İlk olarak REP primeri tarafından oluşturulan bant profili ve BOX primeri tarafından oluşturulan bant profili ayrı ayrı değerlendirildi. Daha sonra her iki primerin oluşturduğu bant profili birlikte değerlendirildi. Bant profillerinin değerlendirilmesi Nei homolojisine göre yapıldı ve bir dendogram (UPGMA) elde edildi. ~ 4000-300 bp arasında değişen toplam 44 bant profili değerlendirildi. Elde edilen verilere göre, hemen hemen aynı izolatlar aynı gruptaydı. Her iki primerin kombinasyonuna dayanan dendrogram, S. aureus suşlarının çoğunun % 62'den fazla benzer olduğunu gösterdi. Bu çalışma, kombine REP ve BOX-PCR tekniğinin S. aureus'ta epidemiyolojiyi araştırmak için hızlı ve güvenilir bir araç sağladığını göstermektedir.

Keywords

• Moleküler tiplendirme
• sığır mastitis
• tekrar eden DNA dizileri
• Staphylococcus aureus

Project Number

108T290

Molecular Typing with BOX-PCR of Staphylococcus aureus Isolated from Milk Samples

Abstract

The aim of this study is to characterize 98 Staphylococcus aureus isolated from bovine subclinical mastitis in Konya region with BOX primer. A total of 28 band profiles ranging from ~ 950-300 bp were generated by the BOX primer. This band profile was evaluated according to Nei homology and a dendogram (UPGMA) was obtained. According to the data obtained, 95 isolates with genetic similarity varying between 50-100% were in the same group. This study demonstrates that the BOX-PCR technique provides a fast and reliable tool to investigate epidemiology in S. aureus.

Keywords

• Molecular typing
• bovine mastitis
• repetitive DNA sequences
• Staphylococcus aureus

Supporting Institution

TUBITAK

Project Number

108T290

Thanks

This research was supported by TUBITAK (Project No: 108T290). We would like to thank Prof. Dr. Uçkun Sait UÇAN for his help in bacterial identification and for the milk collected. In addition, this study was presented as an oral presentation at the 24th National Biochemistry Congress.

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