Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi

Yıl 2019, Cilt: 8 Sayı: 1, 66 - 73, 12.03.2019

Ümit Yırtıcı

https://doi.org/10.17798/bitlisfen.484336

Cited By: 1

Öz

Çiçeklenme döneminde toplanan Centaurea fenzlii Reichardt’ın
toprak üstü kısımları farklı polariteye sahip çözücülerde maserasyon yöntemi
kullanılarak çözüldü ve ekstreleri elde edildi. Çalışmalara metanol ekstresi
ile devam edildi. Metanol ekstresinin toplam fenolik ve flavonoid içerikleri
sırasıyla, 16,72 mg GAE/g ka ve 173,16 mg
KAE/g ka
olarak belirlendi. Antioksidan kapasiteleri demir indirgeyici gücü (FRAP) için 0,256 mmol TE/g
ka, bakır
indirgeyici gücü (CUPRAC) için 0,878 mmol TE/g
ka, ABTS için 0,354 mmol TE/g ka ve DPPH
için 0,661 mmol TE/g ka olarak saptandı. Ayrıca, ekstrelerinin
kolinesteraz, α-amilaz, α-glukozidaz ve tirozinaz enzimlerine karşı inhibe
edici etkileri de belirlendi. Enzim inhibisyon etkisi sırasıyla, α-Glukozidaz için 0,331 mmol AKE/g ka,
α-Amilaz için 0,354 mmol AKE/g ka, AChE için 0,367 mmol GAE/g ka, BChE için
0,878 mmol GAE/g ka ve Tirozinaz için mmol 0,256 KE/g ka olarak bulundu.

Anahtar Kelimeler

Centaurea fenzlii Reichardt, Antioksidan, Enzim İnhibisyonu

Kaynakça

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