PlaBacter: Determination of Microbial Diversity on Plastic Polymers in Topçu Pond

Yıl 2024, Cilt: 2 Sayı: 2, 47 - 55, 29.11.2024

Berkan Yeğen , Emine Tuğçe Saraç Cebeci , Şuranur Güneş , Ezgi Doğru , Sevgi Akdeniz

https://doi.org/10.70500/bjs.1563106

Öz

Climate change is one of the biggest problems of our world, and one of the biggest measures taken to solve the climate crisis is to reduce the consumption of oil and oil derivatives, which are considered among fossil resources. However, with the globalising world and increasing population, oil companies have turned to the plastic and packaging sector in order to meet the increasing demand for plastic consumption. This situation inevitably increases the environmental damage of plastic waste. In particular, the inability to use water resources correctly and the pollution of water by wastes left in the environment adversely affect the lives of living things on earth. Within the scope of the study, it was aimed to determine the microbial diversity living on polyethylene terephthalate (PET), which is known as the raw material of tools such as plastic bottles, food containers, and fishing line, which are left as waste in the environment, especially in rivers and lake shores. In this context, Topçu Pond, which is an important water source in the field of agricultural irrigation in Yozgat province, was selected as the study area. Genomic DNA (gDNA) was isolated from biofilm layers formed on water, sediment, and PET samples collected from the study area. The bacterial communities living in the samples were identified by analysing the 16S rDNA of the gDNAs with the next-generation sequencing method. Thus, we conducted the first microbial diversity study in Topçu Pond and defined the bacterial composition on PET samples in the pond.

Anahtar Kelimeler

Polyethylene terephthalate, 16S rDNA, Next Generation Sequencing, Microbial diversity, Lentic system

Etik Beyan

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Destekleyen Kurum

TÜBİTAK

Proje Numarası

1919B012223936

Teşekkür

-

Kaynakça

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