Relationship Between the Levels of Oxidative Stress in Mesenteric and Peripheral Serum and Clinicopathological Variables in Colorectal Cancer

Abstract

Objective: To explore the differences existing between the levels of oxidative stress in peripheral and mesenteric serum in patients with colorectal cancer. Material and Methods: One hundred fifty patients with colorectal cancer who underwent surgery between May 2005 and March 2010 were prospectively analyzed. The differences between oxidative stress parameters in their peripheral and mesenteric blood were measured. The associations between peripheral and mesenteric levels and the staging and clinicopathological variables were investigated. Results: Oxidative stress parameters were higher in patients with advanced tumor staging (p<0.01), lymph node invasion (p<0.01), and venous invasion (p<0.01). Differences between oxidative stress parameters in peripheral and mesenteric blood samples were also observed. Conclusions: The mesenteric levels of the oxidative stress markers were higher than the peripheral levels in these colorectal cancer patients. Higher levels of these oxidative stress markers are associated with an advanced state of cancer. Turkish Başlık: Kolorektal Kanserli Hastalarda Klinikopatolojik Özellikler ile Periferik ve Mezenterik Serum Oksidatif Stres Düzeyleri Arasındaki İlişki Anahtar Kelimeler: Kolorektal kanser, oksidatif stres, reaktif oksijen Ürünleri Amaç: Kolorektal kanserli hastalarda, periferik ve mezenterik serum oksidatif stres düzeyleri arasındaki farkları göstermek. Gereç ve Yöntemler: Mayıs 2005-Mart 2010 yılları arasında kolorektal kanser nedeniyle cerrahi uygulanan 150 hasta prospektif olarak incelendi. Periferik ve mezenterik kandaki oksidatif stres parametreleri arasındaki farklar ölçüldü. Klinik, patolojik ve tümör evresine göre ilişkileri araştırıldı. Bulgular: İleri evre (p<0.01), lenf nodu invazyonu (p<0.01) ve venöz invazyonu (p<0.01) olan tümörlerde oksidatif stres parametreleri daha yüksekti. Mezenterik ve periferik kan örneklerindeki oksidatif stres parametreleri arasında farklılıklar gözlendi. Sonuç: Kolorektal kanserli hastalarda mezenterik oksidatif stres markır düzeyleri periferik düzeylere göre yüksekti. Oksidatif stres markırlarının yüksek olması ilerlemiş kanser evresi ile ilişkilidir.

Keywords

• Colorectal cancer, oxidative stress, reactive oxygen products

Relationship Between the Levels of Oxidative Stress in Mesenteric and Peripheral Serum and Clinicopathological Variables in Colorectal Cancer

Öz

Kaynakça

1. Nishikawa M. Reactive oxygen species in tumor metastasis. Can- cer Lett 2008;266:53-9. [CrossRef]
2. Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A. Biomarkers of oxidative damage in human disease. Clin Chem 2006;52:601-23. [CrossRef]
3. Toyokuni S. Reactive oxygen species-induced molecular damage and its application in pathology. Pathol Int 1999;49:91-102. [CrossRef]
4. Toyokuni S, Tanaka T, Hattori Y, Nishiyama Y, Yoshida A, Uchida K, et al. Quantitative immunohistochemical determination of 8-hydroxy-2-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogen- esis model. Lab Invest 1997;76:365-74.
5. Girgin F, Karaoglu O, Erkuş M, Tüzün S, Ozütemiz O, Dinçer C, et al. Effects of trimetazidine on oxidant/antioxidant status in trini- trobenzenesulfonic acid-induced chronic colitis. J Toxicol Environ Health A 2000;59:641-52. [CrossRef]
6. Skrzydlewska E, Kozuszko B, Sulkowska M, Bogdan Z, Kozlowski M, Snarska J, et al. Antioxidant potential in esophageal, stomach and colorectal cancers. Hepatogastroenterology 2003;50:126-31.
7. Bridges AB, Fisher TC, Scott N, McLaren M, Belch JJ. Circadian rhythm of white blood cell aggregation and free radical status in healthy vol- unteers. Free Radic Res Commun 1992;16:89-97. [CrossRef]
8. Cakatay U. Protein oxidation parameters in type 2 diabetic pa- tients with good and poor glycaemic control. Diabetes Metab 2005;31:551-7. [CrossRef]

9. Jentzsch AM, Bachmann H, Fürst P, Biesalski HK. Improved anal- ysis of malondialdehyde in human body fluids. Free Radic Biol Med 1996;20:251-6. [CrossRef]
10. Mendoza-Nşñez VM, Ruiz-Ramos M, Sánchez-Rodríguez MA, Re- tana-Ugalde R, Muñoz-Sánchez JL. Aging-related oxidative stress in healthy humans. Tohoku J Exp Med 2007;213:261-8. [CrossRef]
11. Correale M, Arnberg H, Blockx P, Bombardieri E, Castelli M, Encabo G, et al. Clinical profile of a new monoclonal antibody- based immunoassay for tissue polypeptide antigen. Int J Biol Markers 1994;9:231-8.
12. Finlay IG, McArdle CS. The identification of patients at high risk following curative resection for colorectal carcinoma. Br J Surg 1982;69:583-4. [CrossRef]
13. Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem J 1996;313:17-29.
14. Sharma RA, McLelland HR, Hill KA, Ireson CR, Euden SA, Manson MM, et al. Pharmacodynamic and pharmacokinetic study of oral Curcuma extract in patients with colorectal cancer. Clin Cancer Res 2001;7:1894-900.
15. Paduch R, Kandefer-Szerszen M, Piersiak T. The importance of release of proinflammatory cytokines, ROS, and NO in different stages of colon carcinoma growth and metastasis after treatment with cytotoxic drugs. Oncol Res 2010;18:419-36. [CrossRef]
16. Hendrickse CW, Kelly R, Radley S, Donovan IA, Keighley MR, Ne- optolemos JP. Lipid peroxidation and prostoglandins in colorec- tal cancer. Br J Surg 1994;81:1219-23. [CrossRef]
17. Skrzydewska E, Stankiewicz A, Michalak K, Sulkowska M, Zalews- ki B, Piotrowski Z. Antioxidant status and proteolytic-antipro- teolytic balance in colorectal cancer. Folia Histochem Cytobiol 2001;39:98-9.
18. Esterbauer H, Ecki P, Ortner A. Possible mutagens derived from lipids and lipid precursors. Mutant Res 1990;238:223-33.
19. Bosch-Morell F, Flohe L, Marin N, Romero FJ. 4-hydroxynonenal inihibits glutathione peroxidase: protection by glutathione. Free Radical Biol Med 1999;26:1383-7. [CrossRef]
20. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxy-nonenal, malonaldehyde and related aldehydes. Free Radical Biol Med 1991;11:81-128. [CrossRef]