The Evaluation of Saliva Flow Rate, pH, Buffer Capacity, Microbiological Content and Indice of Decayed, Missing and Filled Teeth in Behçet’s Patients

Abstract

Background: Several lines of evidence indicate that oral microbial flora play a critical role in the pathogenesis of Behçet disease. Saliva flow rate, buffer capacity and microorganism content are very important in the maintenance of oral health. Aims: We aimed to evaluate saliva flow rate, pH, buffer capacity and Streptococcus mutans and Lactobacilli content along with the decayed, missing, and filled tooth index in Behçet's patients. Study Design: Case-control study Methods: Forty patients with active Behçet disease [female:male (F/M) 21/19, mean age 31.05±11.08 years] and forty healthy persons (F/M 21/19, mean age 31.03±9.14 years) were included in the study. The oral region was first examined, and the decayed, missing, and filled tooth index was calculated for each person. Stimulated saliva was collected and divided into two separate millimetric tubes to calculate the buffer capacity of the saliva, determine Streptococcus mutans and Lactobacilli levels and measure the pH of the saliva. A Caries Risk Test buffer strip was used to calculate the buffer capacity. Caries Risk Test bacterial kit was used to determine Streptococcus mutans and lactobacilli levels. A pH meter was used to measure the pH of the saliva. Results: The mean saliva pH of the patients was higher than the controls (7.76±0.51, 7.18±0.46, respectively) (p<0.001). The mean level of Streptococcus mutans and Lactobacilli in patients was higher than controls (p<0.05). The mean decayed, missing, and filled tooth index of patients was 9.82±6.59 (range 1-28), while the mean decayed, missing, and filled tooth index of the controls was 6.05±3.35 (range 1-16) (p<0.01). There were no significant differences between the patients and the controls with respect to saliva flow rate and saliva buffer capacity (p>0.05). Conclusion: We think that the maintenance of oral health by effective, regular tooth brushing, regular dental check-ups and dental treatment for Behçet patients is very important for the prevention and therapy of Behçet disease. Turkish Başlık: Behçet Hastalığında Tükürük Akış Hızı, Tamponlama Kapasitesi, Tükürüğün Mikrobiyolojik İçeriği ile Çürük, Kayıp ve Dolgulu Diş İndeksinin Değerlendirilmesi Anahtar Kelimeler: Behçet sendromu, tükürük, DMFT index, Laktobasil, Streptokok mutans. Arka Plan: Oral mikrobiyal floranın Behçet hastalığı patogenezinde önemli rolü olduğuna dair çok sayıda delil vardır. Tükürük akış hızı, tamponlama kapasitesi ve tükrüğün mikrobiyolojik içeriğinin oral sağlığın sürdürülmesinde büyük önemi vardır. Amaç: Konu ile ilgili çalışmaların azlığı ve bu çalışmalardan elde edilen sonuçların yetersizliği nedeniyle biz de çalışmamızda Behçetli hastalarda tükürük akış hızı, tamponlama kapasitesi, tükürüğün Streptokok mutans ve laktobasil içeriği ile çürük, kayıp ve dolgulu diş indekslerini değerlendirmeyi ve bunları sağlıklı bireylerle karşılaştırmayı amaçladık. Çalışma Tasarımı: Olgu kontrol çalışması Yöntemler: Çalışmaya 40 aktif Behçet hastası (kadın/erkek oranı: 21/19, yaş ortalaması: 31.05±11.08 yıl) ile 40 sağlıklı kontrol (kadın/erkek oranı: 21/19 yaş ortalaması:31.03±9.14 yıl) dahil edildi. İlk önce oral mukoza muayenesi yapıldı ve her kişi için çürük, kayıp ve dolgulu diş indeksi hesaplandı. Tükürük tamponlama kapasitesi, ph'sı ile streptokok mutans ve laktobasil tayini için stimüle tükrük toplanarak iki tane milimetrik tüpe bölündü. Tamponlama kapasitesinin ölçülmesinde CRT buffer strip kullanıldı. Streptokok mutans ve laktobasil tayini için CRT bacteria kiti kullanıldı. pH analizi için pH metre kullanıldı. Bulgular: Hastaların tükürük pH ortalaması kontrollerden yüksekti (7.76±0.51, 7.18±0.46, respectively) (p<0.001). Hastaların tükürüğünde S.mutans ve laktobasil üreme oranı kontrollere göre yüksekti (p<0.05). Hastaların çürük, kayıp ve dolgulu diş indeksleri 9.82±6.59 (aralık 1-28), iken kontrollerin çürük, kayıp ve dolgulu diş indeksleri 6.05±3.35 (aralık 1-16) idi. (p<0.01). Tükürük akış hızı ve tükürük tamponlama kapasiteleri açısından hastalar ve kontroller arasındaki istatistiksel olarak anlamlı fark yoktu (p>0.05). Sonuç: Behçet hastalığın önlenmesi ve tedavisinde; etkili ve düzenli diş fırçalama, düzenli diş muayenesi ve tedavileri gibi oral hijyeni sağlayan ve sürdüren önlemlerin alınmasının çok önemli olduğunu düşünüyoruz.

Keywords

• Behçet syndrome, saliva, DMFT index, Lactobacilli, Streptococcus mutans.

The Evaluation of Saliva Flow Rate, pH, Buffer Capacity, Microbiological Content and Indice of Decayed, Missing and Filled Teeth in Behçet’s Patients

Öz

Kaynakça

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