Araştırma Makalesi
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In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii

Yıl 2013, Cilt: 2013 Sayı: 1, 19 - 22, 01.01.2013
https://doi.org/10.5152/balkanmedj.2012.098

Öz

Objective: Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture. Study Design: In-vitro study. Material and Methods: Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures. Results: The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation. Conclusion: These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain. Turkish Başlık: İvermektin ve sulfadiazinin toxoplasma gondıı'ye etkinliklerinin in vitro değerlendirilmesi Anahtar Kelimeler: Toxoplasma gondii, ivermektin, sulfadiazin, in vitro, Hep-2 Amaç: İvermektin ile sulfadiazinin, in vitro T. gondii ile enfekte insan larenks epidermoid karsinom (Hep-2) hücrelerinde anti-toxoplasma aktiviteleri araştırılmıştır. Gereç ve Yöntemler: Antimikrobiyal ajanların anti-Toxoplasma aktiviteleri hücre kültür tabanlı ELISA ile değerlendirilmiştir. Primer antikor olarak tavşan anti-T gondii immunglobulin G sekonder antikor olarak alkalen fosfataz işaretli anti-rabbit immunglobulin G kullanılmıştır. Her ilaç için, optik dansite değerleri ile antimikrobiyal ajan konsantrasyonları arasındaki ilişki regresyon analizi uygulanarak değerlendirilmiştir. Bulgular: İvermektin ve sulfadiazinin inkübasyonun 48. saatinde, %50 inhibitör konsantrasyon (IC50) değerleri sırasıyla 0.2 μg/ml ve 7.3 μg/ml olarak hesaplanmıştır. Ayrıca her iki ilacın test edilen konsantrasyonlarının Hep-2 hücrelerine toksik olmadığı saptanmıştır. Sonuç: Bu sonuçlar; ivermektinin in vitro T.gondii RH suşu takizoit replikasyonunu anlamlı olarak inhibe ettiğini göstermiştir.

Kaynakça

  • Montaya JG, Liesenfeld O. Toxoplasmosis. The Lancet 2004;363:1965-76. [CrossRef]
  • Montoya JG, Kovacs JA, Remington JS. Toxoplasma gondii. In:Mandell GL, Bennett JE, Dolin R, editors. Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases. 6th ed. Philadelphia:Elsevier Press;2008. p.3170-92.
  • Wilson M, Jones LJ, McAuley LB. Toxoplasma. In:Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, editors. Manual of Clinical Microbiology. 9th ed. Washington:ASM Press;2005. p.2070-81. Petersen E. Toxoplasmosis. Seminars in Fetal & Neonatal Medicine 2007;12:214-23. [CrossRef]
  • Merli A, Canessa A, Melioli G. Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture. J Clin Microbiol 1985;21:88-91.
  • Derouin F, Chastang C. Enzyme Immunoassay to assess effect of antimicrobial agents on Toxoplasma gondii in tissue cultures. Antimicrob Agents Chemother 1988;32:303-7. [CrossRef]
  • Diab MR, El-Bahy MM. Toxoplasma gondii:Virulence of tachyzoites in serum free media at different temperatures. Exp Parasitol 2008;118:75-9. [CrossRef]
  • Meneceuri P, Bouldouyre MA, Aubert D, Villena I, Menotti J, Sauvage V, et al. Toxoplasma gondii:In vitro susceptibility of various genotypic strains to pyrimethamine, sulphadiazine and atovaquone. Antimicrob Agents Chemother 2008;52:1269-77. [CrossRef]
  • Derouin F, Chastang C. In vitro effects of folate inhibitors on Toxoplasma gondii. Antimicrob Agents Chemother 1989;33:1753-9. [CrossRef]
  • Guzzo CA, Furtek CI, Chen C, Tipping R, Clineschmidt CM, Sciberras DG, et al. Safety, tolerability, pharmacokinetics of escalating high doses of ivermectin in healthy adult subjects. J Clin Pharmacol 2002;42:1122-33. [CrossRef] 600.00 500 500.00 400 400.00 300 300.00

In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii

Yıl 2013, Cilt: 2013 Sayı: 1, 19 - 22, 01.01.2013
https://doi.org/10.5152/balkanmedj.2012.098

Öz

Kaynakça

  • Montaya JG, Liesenfeld O. Toxoplasmosis. The Lancet 2004;363:1965-76. [CrossRef]
  • Montoya JG, Kovacs JA, Remington JS. Toxoplasma gondii. In:Mandell GL, Bennett JE, Dolin R, editors. Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases. 6th ed. Philadelphia:Elsevier Press;2008. p.3170-92.
  • Wilson M, Jones LJ, McAuley LB. Toxoplasma. In:Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, editors. Manual of Clinical Microbiology. 9th ed. Washington:ASM Press;2005. p.2070-81. Petersen E. Toxoplasmosis. Seminars in Fetal & Neonatal Medicine 2007;12:214-23. [CrossRef]
  • Merli A, Canessa A, Melioli G. Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture. J Clin Microbiol 1985;21:88-91.
  • Derouin F, Chastang C. Enzyme Immunoassay to assess effect of antimicrobial agents on Toxoplasma gondii in tissue cultures. Antimicrob Agents Chemother 1988;32:303-7. [CrossRef]
  • Diab MR, El-Bahy MM. Toxoplasma gondii:Virulence of tachyzoites in serum free media at different temperatures. Exp Parasitol 2008;118:75-9. [CrossRef]
  • Meneceuri P, Bouldouyre MA, Aubert D, Villena I, Menotti J, Sauvage V, et al. Toxoplasma gondii:In vitro susceptibility of various genotypic strains to pyrimethamine, sulphadiazine and atovaquone. Antimicrob Agents Chemother 2008;52:1269-77. [CrossRef]
  • Derouin F, Chastang C. In vitro effects of folate inhibitors on Toxoplasma gondii. Antimicrob Agents Chemother 1989;33:1753-9. [CrossRef]
  • Guzzo CA, Furtek CI, Chen C, Tipping R, Clineschmidt CM, Sciberras DG, et al. Safety, tolerability, pharmacokinetics of escalating high doses of ivermectin in healthy adult subjects. J Clin Pharmacol 2002;42:1122-33. [CrossRef] 600.00 500 500.00 400 400.00 300 300.00
Toplam 9 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Makaleler
Yazarlar

Melek Bilgin Bu kişi benim

Tuba Yıldırım Bu kişi benim

Murat Hökelek Bu kişi benim

Yayımlanma Tarihi 1 Ocak 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 2013 Sayı: 1

Kaynak Göster

APA Bilgin, M., Yıldırım, T., & Hökelek, M. (2013). In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii. Balkan Medical Journal, 2013(1), 19-22. https://doi.org/10.5152/balkanmedj.2012.098
AMA Bilgin M, Yıldırım T, Hökelek M. In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii. Balkan Medical Journal. Ocak 2013;2013(1):19-22. doi:10.5152/balkanmedj.2012.098
Chicago Bilgin, Melek, Tuba Yıldırım, ve Murat Hökelek. “In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma Gondii”. Balkan Medical Journal 2013, sy. 1 (Ocak 2013): 19-22. https://doi.org/10.5152/balkanmedj.2012.098.
EndNote Bilgin M, Yıldırım T, Hökelek M (01 Ocak 2013) In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii. Balkan Medical Journal 2013 1 19–22.
IEEE M. Bilgin, T. Yıldırım, ve M. Hökelek, “In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii”, Balkan Medical Journal, c. 2013, sy. 1, ss. 19–22, 2013, doi: 10.5152/balkanmedj.2012.098.
ISNAD Bilgin, Melek vd. “In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma Gondii”. Balkan Medical Journal 2013/1 (Ocak 2013), 19-22. https://doi.org/10.5152/balkanmedj.2012.098.
JAMA Bilgin M, Yıldırım T, Hökelek M. In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii. Balkan Medical Journal. 2013;2013:19–22.
MLA Bilgin, Melek vd. “In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma Gondii”. Balkan Medical Journal, c. 2013, sy. 1, 2013, ss. 19-22, doi:10.5152/balkanmedj.2012.098.
Vancouver Bilgin M, Yıldırım T, Hökelek M. In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii. Balkan Medical Journal. 2013;2013(1):19-22.