Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits

Yıl 2016, Cilt: 33 Sayı: 6, 639 - 644, 01.11.2016

Yi-shan Wei Dai-he Li Wan-lin Liu Dian-ming Jiang

Öz

Background: Developmental dysplasia of the hip (DDH) is an important factor leading to early adult osteoarthritis. Chondrocyte apoptosis has been proven to be an important factor causing osteoarthritis. Aims: The current study aims to explore whether a rabbit model of developmental dysplasia of the hip through cast immobilization in the legs results in chondrocyte apoptosis.  Study Design: Animal experimentation. Methods: Thirty-two New Zealand white rabbits were divided in three groups with cast plaster-induced dislocation at 2, 4 and 6 weeks. The contralateral hip joint was utilized as a control group. Ten rabbits in each group were sacrificed, and hip specimens were obtained. Bcl-2/Bax, cleaved caspase-3 and cleaved caspase-8 expression were examined by western blot analysis. Chondrocyte apoptosis was analyzed through transmission electron microscopy (TEM) and TUNEL analysis. All experiments were repeated at least three times.  Results: In the experimental group, Bcl-2/Bax, cleaved caspase-3 and cleaved caspase-8 expression were significantly altered. The Bcl-2/Bax ratio decreased with time (all p<0.01), whereas levels of cleaved caspase-3 (p<0.01 and p<0.05) and cleaved caspase-8 (all p<0.05) gradually increased. Chondrocyte apoptosis was observed through transmission electron microscopy (TEM) and TUNEL analysis (p<0.05 at 4 weeks and p<0.01 at 6 weeks).  Conclusion: Prolonged immobilization of rabbit hip caused chondrocyte apoptosis. Reduction of the hip joint may protect chondrocytes from apoptosis, thus preventing secondary osteoarthritis.

Anahtar Kelimeler

Developmental dysplasia of the hip, chondrocyte apoptosis, immoblilization

Kaynakça

• 1. Dezateux C, Rosendahl K. Developmental dysplasia of the hip. Lancet 2007:369:1541-52. [CrossRef]
• 2. Guille JT, Pizzutillo PD, MacEwen GD. Development dysplasia of the hip from birth to six months. J Am Acad Orthop Surg 2000;8:232-42. [CrossRef]
• 3. Panagiotopoulou N, Bitar K, Hart WJ. The association between mode of delivery and developmental dysplasia of the hip in breech infants: a systematic review of 9 cohort studies. Acta Orthop Belg 2012;78:697-702.
• 4. Furnes O, Lie SA, Espehaug B, Vollset SE, Engesaeter LB, Havelin LI. Hip disease and the prognosis of total hip replacements. A review of 53,698 primary total hip replacements reported to the Norwegian arthroplasty register 1987-99. J Bone Joint Surg Br 2001;83:579-86. [CrossRef]
• 5. Chan A, McCaul KA, Cundy PJ, Haan EA, Byron-Scott R. Perinatal risk factors for developmental dysplasia of the hip. Arch Dis Child Neonatal Ed 1997;76:94-100. [CrossRef]
• 6. Sionek A, Czubak J, Kornacka K, Grabowski B. Evaluation of risk factors in developmental dysplasia of the hip in children from multiple pregnancies: results of hip ultrasonography using Graf’s method. Ortop Traumatol Rehabil 2008;10:115-130.
• 7. Stein-Zamir C, Volovik, Rishpon S, Sabi R. Developmental dysplasia of the hip: risk markers, clinical screening and outcome. Pediatr Int 2008;50:341-5. [CrossRef]
• 8. Loening AM, James IE, Levenston ME, Badger AM, Frank EH, Kurz B, et al. Injurious mechanical compression of bovine articular cartilage induces chondrocyte apoptosis. Arch Biochem Biophys 2000;15:205-12. [CrossRef]
• 9. D’Lima DD, Hashimoto S, Chen PC, Colwell CW Jr, Lotz MK. Human chondrocyte apoptosis in response to mechanical injury. Osteoarthritis Cartilage 2001;9:712-9. [CrossRef]
• 10. Lotz M, Hashimoto S, Kühn K. Mechanisms of chondrocyte apoptosis. Osteoarthritis Cartilage 1999;7:389-91. [CrossRef]
• 11. Kouri JB, Aguilera JM, Reyes J, Lozoya KA, González S. Apoptotic chondrocytes from osteoarthrotic human articular cartilage and abnormal calcification of subchondral bone. J Rheumatol 2000;27:1005-19.
• 12. Hashimoto S, Ochs RL, Komiya S, Lotz M. Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum 1998;41:1632-8. [CrossRef]
• 13. Kim HA, Lee YJ, Seong SC, Choe KW, Song YW. Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 2000;27:455-62.
• 14. Thomas CM, Fuller CJ, Whittles CE, Sharif Ml. Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis and Cartilage 2007;15:27-34. [CrossRef]
• 15. Shapiro F, Connolly S, Zurakowski D, Flynn E, Jaramillo D. Acetabular changes associated with avascular necrosis of the femoral head in a piglet model. Bone Joint Res 2014;3:130-8. [CrossRef]
• 16. Klisic PJ. Congenital dislocation of the hip-a misleading term: brief report. J Bone Joint Surg Br 1989;71:136.