Role of Trace Elements for Oxidative Status and Quality of Human Sperm

Yıl 2017, Cilt: 34 Sayı: 4, 343 - 348, 01.07.2017

Galina Nenkova Lubomir Petrov Albena Alexandrova

Öz

Background: Oxidative stress affects sperm quality negatively. To maintain the pro/antioxidant balance, some metal ions (e.g. copper, zink, iron, selenium), which are co-factors of the antioxidant enzymes, are essential. However, iron and copper could act as prooxidants inducing oxidative damage of spermatozoa. Aims: To reveal a possible correlation between the concentrations of some metal ions (iron, copper, zinc, and selenium) in human seminal plasma, oxidative stress, assessed by malondialdehyde and total glutathione levels, and semen quality, assessed by the parameters count, motility, and morphology. Study Design: Descriptive study. Methods: The semen analysis for volume, count, and motility was performed according to World Health Organization (2010) guidelines, using computer-assisted semen analysis. For the determination of spermatozoa morphology, a SpermBlue staining method was applied. Depending on their parameters, the sperm samples were categorized into normozoospermic, teratozoospermic, asthenoteratozoospermic, and oligoteratozoospermic. The seminal plasma content of iron, copper, zinc, and selenium was estimated by atomic absorption spectroscopy. The malondialdehyde and total glutathione levels were quantified spectrophotometrically. Results: In the groups with poor sperm quality, the levels of Fe were higher, whereas those of Zn and Se were significantly lower than in the normozoospermic group. In all groups with poor sperm quality, increased levels of malondialdehyde and decreased glutathione levels were detected as evidence of oxidative stress occurrence. All these differences are most pronounced in the asthenoteratozoospermic group where values differ nearly twice as much compared to the normozoospermic group. The Fe concentration correlated positively with the malondialdehyde (r=0.666, p=0.018), whereas it showed a negative correlation with the level of total glutathione (r=-0.689, p=0.013). The total glutathione level correlated positively with the sperm motility (r=0.589, p=0.044). Conclusion: The elevated levels of Fe and the reduced Se levels are associated with sperm damage. The changes in the concentrations of the trace elements in human seminal plasma may be related to sperm quality since they are involved in the maintenance of the pro-/antioxidative balance in ejaculate.

Anahtar Kelimeler

Metal ions, oxidative status, sperm

Kaynakça

• 1. Boivin J, Bunting L, Collins JA, Nygren KG. International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Human Reprod 2007;22:1506-12.
• 2. Agarwal A, Prabakaran SA. Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology. Indian J Exp Biol 2005;43:963-74.
• 3. Aitken RJ, Buckingham D, Harkiss D. Use of a xanthine oxidase free radical generating system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa. J Reprod Fertil 1993;97:441-50.
• 4. Gallardo JM. Evaluation of antioxidant system in normal semen. Rev Invest Clin 2007;59:42-7.
• 5. Gavella M, Lipovac V, Vučić M, Ročić B. Evaluation of ascorbate and urate antioxidant capacity in human semen. Andrologia 1997;29:29-35.
• 6. Bhardwaj A, Verma A, Majumdar S, Khanduja KL. Status of vitamin E and reduced glutathione in semen of oligozoospermic and azoospermic patients. Asian J Androl 2000;2:225-8.
• 7. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002;29:817-27.
• 8. Griveau, JF, Dumont E, Renard B, Callegari JP, Lannou D. Reactive oxygen species, lipid peroxidation and enzymatic defense systems in human spermatozoa. J Reprod Fertil 1995;103:17-26.
• 9. World Health Organization. WHO laboratory manual for the examination and processing of human semen - 5th ed. Geneva, CH: World Health Organization; 2010.
• 10. Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehringer S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988;49:112-7.
• 11. Hunter F Jr, Gebinski JM, Hoffstein PE, Weinstein J, Scott A. Swelling and lysis of rat liver mitochondria by ferrous ions. J Biol Chem 1963;238:828- 35.
• 12. Akerboom TP, Sies H. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 1981;77:373-82.
• 13. Silva J. J. R. F. D., Williams R. J. P. The Biological Chemistry of the Elements, Clarendon Press: Oxford; 1991.
• 14. Khosrowbeygi A, Zarghami N, Deldar Y. Correlation between sperm quality parameters and seminal plasma antioxidants status. Iranian Journal of Reproductive Medicine 2004;2:58-64.
• 15. Skandhan KP, Mazumdar BN, Sumangala B. Study into the iron content of seminal plasma in normal and infertile subjects. Urologia 2012;79:54-7.
• 16. Charlton RW, Bothwell TH. In: Thompson RGS Woodton IPP, Eds. Idiopathic haemochromatosis and related iron storage disorders. Biochemical disorders in human disease, 3-rd edition J & A Churchill, London; 1970:201-14.
• 17. Huang YL, Tseng WS, Cheng SY, Lin TH. Trace elements and lipid peroxidation in human seminal plasma. Biol Trace Elem Res 2000;76:207- 15.
• 18. Huang YL, Tseng WC, Lin TH. In vitro effects of metal ions (Fe2+, Mn2+, Pb2+) on sperm motility and lipid peroxidation in human semen. J Toxicol Environ Health A 2001;62:259-67.
• 19. Aitken RJ, Clarkson JS, Fishel S. Generation of reactive oxygen species, lipid peroxidation, and oxygen species, lipid peroxidation, and human sperm function. Biol Reprod 1989;41:183-97.
• 20. Atig F, Raffa M, Habib BA, Kerkeni A, Saad A, Ajina M. Impact of seminal trace element and glutathione levels on semen quality of Tunisian infertile men. BMC Urol 2012;12:6.
• 21. Akinloye O, Arowojolu AO, Shittu OB, Adejuwon CA, Osotimehin B. Selenium status of idiopathic infertile Nigerian males. Biol Trace Elem Res 2005;104:9-18.
• 22. Colagar AH, Marzony ET, Chaichi MJ. Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutr Res 2009;29:82-8.
• 23. Xu B, Chia SE, Tsakok M, Ong CN. Trace elements in blood and seminal plasma and their relationship to sperm quality. Reprod Toxicol 1993;7:613- 708.
• 24. Powell SR. Antioxidant properties of zinc. J Nutr 2000;130:1447-54.
• 25. Shiva M, Gautam AK, Verma Y, Shivgotra V, Doshi H, Kumar S. Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clin Biochem 2011;44:319-24.
• 26. Skandhan KP. Review on copper in male reproduction and contraception. Rev Fr Gynecol Obstet 1992;87:594-8.
• 27. Abdul Rasheed OF. Association between seminal plasma copper and magnesium levels with oxidative stress in Iraqi infertile men. Oman Med J 2010;25:168-72.
• 28. Buhling KJ, Laakmann E. The effect of micronutrient supplements on male fertility. Curr Opin Obstet Gynecol 2014;26:199-209.