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Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary

Yıl 2017, Cilt: 34 Sayı: 5, 444 - 449, 01.09.2017

Öz

Apigenin is a plant-derived compound belonging to the flavone class, which possess antioxidant, free-radical-scavenging and anti-inflammatory properties. Administration of apigenin has no significant protective effect on ovarian reserve and tissue damage in ovarian ischemia/reperfusion injury Background: Apigenin is a plant-derived compound belonging to the flavone class, which possess antioxidant, free-radical-scavenging and anti-inflammatory properties. Aims: To address the effects of apigenin on serum anti-mullerian hormone levels, tissue oxidative stress parameters and histopathological changes in ovarian ischemia/reperfusion injury. Study Design: Animal experiment. Methods: Twenty-eight female Wistar albino rats were randomly separated into four sections: Sham operation (group 1), ischemia/reperfusion plus saline (group 2), ischemia/reperfusion plus dimethyl sulfoxide (group 3) and ischemia/reperfusion plus apigenin (group 4). In all ischemia/reperfusion groups, a bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion. A single dose of 15 mg/kg apigenin was given intraperitoneally 60 min before reperfusion in group 4. After 3-h of reperfusion, both ovaries were removed, and blood samples were collected. The main outcome measures were serum anti-mullerian hormone levels, ovarian tissue malondialdehyde, total nitric oxide, Cu/Zn superoxide dismutase, catalase and glutathione levels and histopathological damage scores. Results: The ovarian tissue nitric oxide level was significantly lower, and the glutathione level was significantly higher in group 4 compared with groups 2 and 3. There was no significant difference in anti-mullerian hormone levels among the three ischemia/reperfusion groups. The histopathological damage score was lower in group 4 than in groups 2 and 3 (p>0.05). Conclusion: Administration of apigenin has no significant protective effect on ovarian reserve and tissue damage in ovarian ischemia/reperfusion injury.

Kaynakça

  • 1. Houry D, Abbott JT. Ovarian torsion: a fifteen-year review. Ann Emerg Med 2001;38:156-9.
  • 2. Sasaki KJ, Miller CE. Adnexal torsion: review of the literature. J Minim Invasive Gynecol 2014;21:196-202.
  • 3. Köleli I. Mean Platelet Volume in Early Diagnosis of Adnexal Torsion. Balkan Med J 2015;32:410-3.
  • 4. Damigos E, Johns J, Ross J. An update on the diagnosis and management of ovarian torsion. The Obstetrician & Gynaecologist 2012;14:229-36.
  • 5. Ozler A, Turgut A, Soydinç HE, Sak ME, Evsen MS, Alabalik U, et al. The biochemical and histologic effects of adnexal torsion and early surgical intervention to unwind detorsion on ovarian reserve: an experimental study. Reprod Sci 2013;20:1349-55.
  • 6. Li C, Jackson RM. Reactive species mechanisms of cellular hypoxiareoxygenation injury. Am J Physiol Cell Physiol 2002;282:C227-41.
  • 7. Baarends WM, Uilenbroek JT, Kramer P, Hoogerbrugge JW, van Leeuwen EC, Themmen AP, et al. Anti-müllerian hormone and anti-müllerian hormone type II receptor messenger ribonucleic acid expression in rat ovaries during postnatal development, the estrous cycle, and gonadotropininduced follicle growth. Endocrinology 1995;136:4951-62.
  • 8. Bonilla-Musoles F, Castillo JC, Caballero O, Pérez-Panades J, Bonilla F Jr, Dolz M, et al. Predicting ovarian reserve and reproductive outcome using antimüllerian hormone (AMH) and antral follicle count (AFC) in patients with previous assisted reproduction technique (ART) failure. Clin Exp Obstet Gynecol 2012;39:13-8.
  • 9. Huang CS, Lii CK, Lin AH, Yeh YW, Yao HT, Li CC, et al. Protection by chrysin, apigenin, and luteolin against oxidative stress is mediated by the Nrf2-dependent up-regulation of heme oxygenase 1 and glutamate cysteine ligase in rat primary hepatocytes. Arch Toxicol 2013;87:167-78.
  • 10. Nicholas C, Batra S, Vargo MA, Voss OH, Gavrilin MA, Wewers MD, et al. Apigenin blocks lipopolysaccharide-induced lethality in vivo and proinflammatory cytokines expression by inactivating NFkappaB through the suppression of p65 phosphorylation. J Immunol 2007;179:7121-7.
  • 11. Liu C, Tu FX, Chen X. [Neuroprotective effects of apigenin on acute transient focal cerebral ischemia-reperfusion injury in rats]. Zhong Yao Cai 2008;31:870-3.
  • 12. Yang X, Yang J, Hu J, Li X, Zhang X, Li Z. Apigenin attenuates myocardial ischemia/reperfusion injury via the inactivation of p38 mitogen-activated protein kinase. Mol Med Rep 2015;12:6873-8.
  • 13. Tsalkidou EG, Tsaroucha AK, Chatzaki E, Lambropoulou M, Papachristou F, Trypsianis G, et al. The effects of apigenin on the expression of Fas/ FasL apoptotic pathway in warm liver ischemia-reperfusion injury in rats. Biomed Res Int 2014;2014:157216.
  • 14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • 15. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988;34:497-500.
  • 16. Beutler E, Duron O, Kelly MB. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;51:882-8.
  • 17. Guven S, Muci E, Unsal MA, Yulug E, Alver A, Kadioglu Duman M, et al. The effects of carbon dioxide pneumoperitoneum on ovarian blood flow, oxidative stress markers, and morphology during laparoscopy: a rabbit model. Fertil Steril 2010;93:1327-32.
  • 18. Bulger EM, Maier RV. Antioxidants in critical illness. Arch Surg 2001;136:1201-7.
  • 19. Cosar E, Sahin FK, Köken G, Toy H, Basarali K, Büyükbas S. The protective effect of alpha-lipoic acid in experimental ovarian ischaemiareperfusion injury. Aust N Z J Obstet Gynaecol 2007;47:499-503.
  • 20. Bakan V, Ciralik H, Tolun FI, Atli Y, Mil A, Oztürk S. Protective effect of erythropoietin on torsion/detorsion injury in rat model. J Pediatr Surg 2009;44:1988-94.
  • 21. Ozler A, Turgut A, Görük NY, Alabalik U, Basarali MK, Akdemir F. Evaluation of the protective effects of CoQ₁₀ on ovarian I/R injury: an experimental study. Gynecol Obstet Invest 2013;76:100-6.
  • 22. Hu J, Li Z, Xu LT, Sun AJ, Fu XY, Zhang L, et al. Protective effect of apigenin on ischemia/reperfusion injury of the isolated rat heart. Cardiovasc Toxicol 2015;15:241-9.
  • 23. Cai M, Ma Y, Zhang W, Wang S, Wang Y, Tian L, et al. Apigenin-7-O-βD-(-6''-p-coumaroyl)-Glucopyranoside Treatment Elicits Neuroprotective Effect against Experimental Ischemic Stroke. Int J Biol Sci 2016;12:42-52.
  • 24. Gencer M, Karaca T, Güngör AN, Hacıvelioğlu SÖ, Demirtaş S, Turkon H, et al. The protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury. Eur J Obstet Gynecol Reprod Biol 2014;177:135-40.
  • 25. Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochem Pharmacol 2003;65:1035-41.
  • 26. Bozkurt S, Arikan DC, Kurutas EB, Sayar H, Okumus M, Coskun A, et al. Selenium has a protective effect on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation. J Pediatr Surg 2012;47:1735-41.
  • 27. Mao XY, Yu J, Liu ZQ, Zhou HH. Apigenin attenuates diabetes-associated cognitive decline in rats via suppressing oxidative stress and nitric oxide synthase pathway. Int J Clin Exp Med 2015;8:15506-13.
  • 28. Wang IK, Lin-Shiau SY, Lin JK. Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukaemia HL-60 cells. Eur J Cancer 1999;35:1517-25.
  • 29. Kaya C, Turgut H, Cengiz H, Turan A, Ekin M, Yaşar L. Effect of detorsion alone and in combination with enoxaparin therapy on ovarian reserve and serum antimüllerian hormone levels in a rat ovarian torsion model. Fertil Steril 2014;102:878-84.
Yıl 2017, Cilt: 34 Sayı: 5, 444 - 449, 01.09.2017

Öz

Kaynakça

  • 1. Houry D, Abbott JT. Ovarian torsion: a fifteen-year review. Ann Emerg Med 2001;38:156-9.
  • 2. Sasaki KJ, Miller CE. Adnexal torsion: review of the literature. J Minim Invasive Gynecol 2014;21:196-202.
  • 3. Köleli I. Mean Platelet Volume in Early Diagnosis of Adnexal Torsion. Balkan Med J 2015;32:410-3.
  • 4. Damigos E, Johns J, Ross J. An update on the diagnosis and management of ovarian torsion. The Obstetrician & Gynaecologist 2012;14:229-36.
  • 5. Ozler A, Turgut A, Soydinç HE, Sak ME, Evsen MS, Alabalik U, et al. The biochemical and histologic effects of adnexal torsion and early surgical intervention to unwind detorsion on ovarian reserve: an experimental study. Reprod Sci 2013;20:1349-55.
  • 6. Li C, Jackson RM. Reactive species mechanisms of cellular hypoxiareoxygenation injury. Am J Physiol Cell Physiol 2002;282:C227-41.
  • 7. Baarends WM, Uilenbroek JT, Kramer P, Hoogerbrugge JW, van Leeuwen EC, Themmen AP, et al. Anti-müllerian hormone and anti-müllerian hormone type II receptor messenger ribonucleic acid expression in rat ovaries during postnatal development, the estrous cycle, and gonadotropininduced follicle growth. Endocrinology 1995;136:4951-62.
  • 8. Bonilla-Musoles F, Castillo JC, Caballero O, Pérez-Panades J, Bonilla F Jr, Dolz M, et al. Predicting ovarian reserve and reproductive outcome using antimüllerian hormone (AMH) and antral follicle count (AFC) in patients with previous assisted reproduction technique (ART) failure. Clin Exp Obstet Gynecol 2012;39:13-8.
  • 9. Huang CS, Lii CK, Lin AH, Yeh YW, Yao HT, Li CC, et al. Protection by chrysin, apigenin, and luteolin against oxidative stress is mediated by the Nrf2-dependent up-regulation of heme oxygenase 1 and glutamate cysteine ligase in rat primary hepatocytes. Arch Toxicol 2013;87:167-78.
  • 10. Nicholas C, Batra S, Vargo MA, Voss OH, Gavrilin MA, Wewers MD, et al. Apigenin blocks lipopolysaccharide-induced lethality in vivo and proinflammatory cytokines expression by inactivating NFkappaB through the suppression of p65 phosphorylation. J Immunol 2007;179:7121-7.
  • 11. Liu C, Tu FX, Chen X. [Neuroprotective effects of apigenin on acute transient focal cerebral ischemia-reperfusion injury in rats]. Zhong Yao Cai 2008;31:870-3.
  • 12. Yang X, Yang J, Hu J, Li X, Zhang X, Li Z. Apigenin attenuates myocardial ischemia/reperfusion injury via the inactivation of p38 mitogen-activated protein kinase. Mol Med Rep 2015;12:6873-8.
  • 13. Tsalkidou EG, Tsaroucha AK, Chatzaki E, Lambropoulou M, Papachristou F, Trypsianis G, et al. The effects of apigenin on the expression of Fas/ FasL apoptotic pathway in warm liver ischemia-reperfusion injury in rats. Biomed Res Int 2014;2014:157216.
  • 14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • 15. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988;34:497-500.
  • 16. Beutler E, Duron O, Kelly MB. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;51:882-8.
  • 17. Guven S, Muci E, Unsal MA, Yulug E, Alver A, Kadioglu Duman M, et al. The effects of carbon dioxide pneumoperitoneum on ovarian blood flow, oxidative stress markers, and morphology during laparoscopy: a rabbit model. Fertil Steril 2010;93:1327-32.
  • 18. Bulger EM, Maier RV. Antioxidants in critical illness. Arch Surg 2001;136:1201-7.
  • 19. Cosar E, Sahin FK, Köken G, Toy H, Basarali K, Büyükbas S. The protective effect of alpha-lipoic acid in experimental ovarian ischaemiareperfusion injury. Aust N Z J Obstet Gynaecol 2007;47:499-503.
  • 20. Bakan V, Ciralik H, Tolun FI, Atli Y, Mil A, Oztürk S. Protective effect of erythropoietin on torsion/detorsion injury in rat model. J Pediatr Surg 2009;44:1988-94.
  • 21. Ozler A, Turgut A, Görük NY, Alabalik U, Basarali MK, Akdemir F. Evaluation of the protective effects of CoQ₁₀ on ovarian I/R injury: an experimental study. Gynecol Obstet Invest 2013;76:100-6.
  • 22. Hu J, Li Z, Xu LT, Sun AJ, Fu XY, Zhang L, et al. Protective effect of apigenin on ischemia/reperfusion injury of the isolated rat heart. Cardiovasc Toxicol 2015;15:241-9.
  • 23. Cai M, Ma Y, Zhang W, Wang S, Wang Y, Tian L, et al. Apigenin-7-O-βD-(-6''-p-coumaroyl)-Glucopyranoside Treatment Elicits Neuroprotective Effect against Experimental Ischemic Stroke. Int J Biol Sci 2016;12:42-52.
  • 24. Gencer M, Karaca T, Güngör AN, Hacıvelioğlu SÖ, Demirtaş S, Turkon H, et al. The protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury. Eur J Obstet Gynecol Reprod Biol 2014;177:135-40.
  • 25. Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochem Pharmacol 2003;65:1035-41.
  • 26. Bozkurt S, Arikan DC, Kurutas EB, Sayar H, Okumus M, Coskun A, et al. Selenium has a protective effect on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation. J Pediatr Surg 2012;47:1735-41.
  • 27. Mao XY, Yu J, Liu ZQ, Zhou HH. Apigenin attenuates diabetes-associated cognitive decline in rats via suppressing oxidative stress and nitric oxide synthase pathway. Int J Clin Exp Med 2015;8:15506-13.
  • 28. Wang IK, Lin-Shiau SY, Lin JK. Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukaemia HL-60 cells. Eur J Cancer 1999;35:1517-25.
  • 29. Kaya C, Turgut H, Cengiz H, Turan A, Ekin M, Yaşar L. Effect of detorsion alone and in combination with enoxaparin therapy on ovarian reserve and serum antimüllerian hormone levels in a rat ovarian torsion model. Fertil Steril 2014;102:878-84.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA65AB48SC
Bölüm Araştırma Makalesi
Yazarlar

Zeynep Soyman Bu kişi benim

Sefa Kelekçi Bu kişi benim

Veysel Sal Bu kişi benim

Osman Şevket Bu kişi benim

Nihan Bayındır Bu kişi benim

Hafize Uzun Bu kişi benim

Yayımlanma Tarihi 1 Eylül 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 34 Sayı: 5

Kaynak Göster

APA Soyman, Z., Kelekçi, S., Sal, V., Şevket, O., vd. (2017). Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary. Balkan Medical Journal, 34(5), 444-449.
AMA Soyman Z, Kelekçi S, Sal V, Şevket O, Bayındır N, Uzun H. Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary. Balkan Medical Journal. Eylül 2017;34(5):444-449.
Chicago Soyman, Zeynep, Sefa Kelekçi, Veysel Sal, Osman Şevket, Nihan Bayındır, ve Hafize Uzun. “Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary”. Balkan Medical Journal 34, sy. 5 (Eylül 2017): 444-49.
EndNote Soyman Z, Kelekçi S, Sal V, Şevket O, Bayındır N, Uzun H (01 Eylül 2017) Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary. Balkan Medical Journal 34 5 444–449.
IEEE Z. Soyman, S. Kelekçi, V. Sal, O. Şevket, N. Bayındır, ve H. Uzun, “Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary”, Balkan Medical Journal, c. 34, sy. 5, ss. 444–449, 2017.
ISNAD Soyman, Zeynep vd. “Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary”. Balkan Medical Journal 34/5 (Eylül 2017), 444-449.
JAMA Soyman Z, Kelekçi S, Sal V, Şevket O, Bayındır N, Uzun H. Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary. Balkan Medical Journal. 2017;34:444–449.
MLA Soyman, Zeynep vd. “Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary”. Balkan Medical Journal, c. 34, sy. 5, 2017, ss. 444-9.
Vancouver Soyman Z, Kelekçi S, Sal V, Şevket O, Bayındır N, Uzun H. Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary. Balkan Medical Journal. 2017;34(5):444-9.