Toxicity of hemp (Cannabis sativa L.) extract and essential oil on sunn pest [Eurygaster spp. (Hem: Scutelleridae)]

Yıl 2024, , 78 - 85, 08.07.2024

Şeyda Şimşek Gamze Pekbey Güngör Yılmaz Talip Koçer Tuğba Çakar

https://doi.org/10.59128/bojans.1500806

Öz

In this study, the contact toxicity of essential oil and methanol extracts obtained from Narlısaray, Futura-75 and Kavacık genotypes of Cannabis sativa L. (Cannabacea) on sunnose adults and nymphs was investigated. Contact toxicity of the extracts was tested by topical application using a microapplicator against the sunken, adult and nymph stages. Concentrations were prepared by dissolving the obtained extracts with a 50% acetone/water mixture and the essential oils with acetone, and the control group was also treated with these solvents. As a result of the contact effect study, Narlısaray genotype extract showed the highest effectiveness against adults (78.2%) and nymphs (80.7%) at 20% (w/v) concentration at the 72nd hour. Kavacık genotype showed the lowest contact toxicity.

Anahtar Kelimeler

Cannabis sativa, Eurygaster spp, Biological activity, Contact toxicity

Proje Numarası

6608-ZF/20-420

Kenevir (Cannabis sativa L.) Ekstrakt ve Uçucu Yağının Süne [Eurygaster spp. (Hem: Scutelleridae)] Üzerindeki Toksisitesi

Öz

Yapılan çalışma ile Cannabis sativa L. (Cannabacea)’nın Narlısaray, Futura-75 ve Kavacık genotiplerinden elde edilen uçucu yağ ve metanol ekstraktlarının süne, ergin ve nimfleri üzerindeki kontakt toksisitesi araştırılmıştır. Ekstraktların kontakt toksisitesi süne, ergin ve nimf dönemlerine karşı mikroaplikatör kullanılarak topikal uygulama ile test edilmiştir. Elde edilen ekstraktlar %50 aseton/su karışımı ile uçucu yağlar ise aseton ile çözülerek konsantrasyonlar hazırlanmıştır ve kontrol grubu da bu çözücüler ile muamele edilmiştir. Çalışmada kontak etki çalışması sonucunda 72. saatte en yüksek etkinliği %20 (w/v) konsantrasyonda ergin (%78,2) ve nimflere (%80,7) karşı Narlısaray genotipi ekstraktı göstermiştir. En düşük kontak toksisiteyi ise Kavacık genotipi göstermiştir.

Anahtar Kelimeler

Cannabis sativa, Eurygaster spp, Biyolojik aktivite, Kontakt toksisite

Proje Numarası

6608-ZF/20-420

Kaynakça

• Alkan, M. and Gökçe, A. (2012). Toxic and behavioural effects of Tanacetum abrotanifolium L. DRUCE (Asteraceae) stem and flower extracts on Sitophilus granarius and Sitophilus oryzae (Col., Curculionidae). Turkish Journal of Entomology, 36(3), 377-390.
• Babaroğlu N. E. (2006) Süne [Eurygaster spp. (Hemiptera: Scutelleridae)] Mücadelesinde Kullanılan Bazı İlaçların Orta Anadolu Bölgesinde Süne Yumurta Parazitoitleri Trissolcus spp. (Hymenoptera: Scelionidae)'ne Etkileri Üzerinde Araştırmalar, Doktora, Ankara Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı, Ankara, 128 s.
• Bedini, S., Flamini, G., Cosci, F., Ascrizzi, R., Benelli, G., and Conti, B. (2016). Cannabis sativa and Humulus lupulus essential oils as novel control tools against the invasive mosquito Aedes albopictus and fresh water snail Physella acuta. Industrial Crops and Products, 85, 318-323. https://doi.org/10.1016/j.indcrop.2016.03.008
• Ben Khedher, M. R., Ben Khedher, S., Chaieb, I., Tounsi, S., and Hammami, M. (2017). Chemical composition and biological activities of Salvia officinalis essential oil from Tunisia. EXCLI Journal, 16, 160-173. https://doi.org/10.17179/EXCLI2016-832
• Bedini, S., Flamini, G., Cosci, F., Ascrizzi, R., Benelli, G., and Conti, B. (2016). Cannabis sativa and Humulus lupulus essential oils as novel control tools against the invasive mosquito Aedes albopictus and fresh water snail Physella acuta. Industrial crops and products, 85, 318-323.
• Benelli, G., Pavela, R., Petrelli, R., Cappellacci, L., Santini, G., Fiorini, D., Sut, S., Dall’Acqua, S., Canale, A., and Maggi, F. (2018). The essential oil from industrial hemp (Cannabis sativa L.) by-products as an effective tool for insect pest management in organic crops. Industrial Crops and Products, 122, 308-315. https://doi.org/10.1016/j.indcrop.2018.05.032
• Borhade, S. S. (2013). Chemical Composition and Characterization of Hemp (Cannabis sativa) Seed oil and essential fatty acids by HPLC Method. Archives of applied science research, 5(1), 5-8.
• Corsi, L., Pellati, F., Brighenti, V., Plessi, N., and Benvenuti, S. (2019). Chemical composition and in vitro neuroprotective activity of fibre-type Cannabis sativa L.(hemp). Current Bioactive Compounds, 15(2), 201-210.
• Elma, F. N. ve Alaoğlu, Ö. (2014). Bazı bitki ekstraktlarının Avrupa Sünesi [Eurygaster maura L. (Heteroptera: Scutellaridae)]’nin farklı dönem nimflerine toksik etkileri. Türkiye Entomoloji Dergisi, 38(2), Art. 2.
• Fountain, E.D. and Wratten, S.D. (2013). Conservation biological control and biopesticides in agricultural. Encycl. Ecol. 1, 377-381.
• Isman, M. B., Wilson, J. A., and Bradbury, R. (2008). Insecticidal activities of commercial rosemary oils (Rosmarinus officinalis.) against larvae of Pseudaletia unipuncta. and Trichoplusia ni. In relation to their chemical compositions. Pharmaceutical Biology, 46(1-2), 82-87.
• Kornpointner, C., Martinez, A. S., Marinovic, S., Haselmair-Gosch, C., Jamnik, P., Schröder, K., ... and Halbwirth, H. (2021). Chemical composition and antioxidant potential of Cannabis sativa L. roots. Industrial Crops and Products, 165, 113422.
• Kumar, S. (2012). Biopesticides: A Need for Food and Environmental Safety. J. Biofertil. Biopestici. 3, 107.
• Mansour Ismail, S. (2022). Fumigant residual impacts of Melaleuca alternifolia (Maid. & Betche) Cheel. (Myrtales: Myrtaceae), terpinen-4-ol, and γ-terpinene on Sitophilus oryzae L. (Coleoptera: Curculionidae) on germination of wheat seeds. Journal of Plant Protection Research, 62(3), 258-264.
• Mantzoukas, S., Kalyvas, N., Ntoukas, A., Lagogiannis, I., Farsalinos, K., Eliopoulos, P. A., and Poulas, K. (2020). Combined toxicity of cannabidiol oil with three bio-pesticides against adults of Sitophilus zeamais, Rhyzopertha dominica, Prostephanus truncatus and Trogoderma granarium. International Journal of Environmental Research and Public Health, 17(18), 6664.
• Pavela, R., Kaffkova, K., and Kumšta, M. (2014). Chemical composition and larvicidal activity of essential oils from different Mentha L. and Pulegium species against Culex quinquefasciatus say (Diptera: Culicidae). Plant Protection Science, 50(1).
• Pavela, R. and Sedlák, P. (2018). Post-application temperature as a factor influencing the insecticidal activity of essential oil from Thymus vulgaris. Industrial Crops and Products, 113, 46-49. https://doi.org/10.1016/j.indcrop.2018.01.021
• Peña-Cerda, M., Arancibia-Radich, J., Valenzuela-Bustamante, P., Pérez-Arancibia, R., Barriga, A., Seguel, I., ... and Delporte, C. (2017). Phenolic composition and antioxidant capacity of Ugni molinae Turcz. leaves of different genotypes. Food chemistry, 215, 219-227.
• Pieracci, Y., Ascrizzi, R., Terreni, V., Pistelli, L., Flamini, G., Bassolino, L., ... and Paris, R. (2021). Essential oil of Cannabis sativa L: Comparison of yield and chemical composition of 11 hemp genotypes. Molecules, 26(13), 4080.
• Riyaz, M., Mathew, P., Zuber, S. M., and Rather, G. A. (2022). Botanical Pesticides for an Eco-Friendly and Sustainable Agriculture: New Challenges and Prospects. Sustainable Agriculture, 69-96.
• Xu, J., Bai, M., Song, H., Yang, L., Zhu, D., and Liu, H. (2022). Hemp (Cannabis sativa subsp. sativa) Chemical composition and the application of hempseeds in food formulations. Plant Foods for Human Nutrition, 77(4), 504-513.
• Žabka, M., Pavela, R., Kovaříková, K., Tříska, J., Vrchotová, N., and Bednář, J. (2021). Antifungal and Insecticidal Potential of the Essential Oil from Ocimum sanctum L. against Dangerous Fungal and Insect Species and Its Safety for Non-Target Useful Soil Species Eisenia fetida (Savigny, 1826). Plants, 10(10), 2180. https://doi.org/10.3390/plants10102180