Potasyum metaborat'a ait in vitro biyolojik aktiviteler; antioksidant, antimikrobiyal ve antibiyofilm özellikleri

Öz

Bu çalışmada potasyum metaboratın (KBO2) antioksidan, antimikrobiyal ve antibiyofilm aktiviteleri incelenmiştir. Potasyum metaboratın antioksidan aktivitesi beta-karoten ağartma (BCB) analizi ve hidroksil radikal giderim aktivitesi olarak belirlenmiştir. Potasyum metaboratın antimikrobial aktivitesi, seçili Gram-pozitif bakteri, Gram-negatif bakteri ve maya suşlarına karşı tüp dilüsyon metodu ile incelenmiştir. Potasyum metaboratın test edilen mikroorganizmaların biyofilm oluşturma özelliği üzerine etkinliği MTT (3- [4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide) testi ile belirlenmiştir. Biyofilm inhibisyon kapasitesi ayrıca Taramalı Elektron Mikroskobu (SEM) ile görüntülenmiştir. Potasyum metaboratın hidroksil radikallerini giderim kapasitesi 100 mM konsantrasyonda % 71.13 olarak hesaplanmıştır. Aynı konsatrasyonda BCB aktivitesi daha yüksek sonuç vererek % 86.96 olrak belirlenmiştir. Minimum İnhibisyon Konsantrasyonu (MİK) hesaplamalarına göre potasyum metaborat 62.5 mM konsantrasyonda C. albicans, S. aureus ve E. coli üremesini inhibe ederken, 31.25 mM konsatrasyonda B. subtilis, 125 mM konsantrasyonda ise P. aeruginosa üremesini inhibe etmiştir. MİK değeri uygulanan antibiyofilm aktivite analizi sonucuna göre en yüksek aktivite % 90.18 biyofilm inhibisyon değeri ile C. albicans’ a karşı görülmüştür. Sonuç olarak potasyum metaboratın güçlü biyolojik aktivitelere sahip olduğu ve biyomedikal ve çevresel çözümlerde etkili bir şekilde kullanılabileceği görülmüştür.

Anahtar Kelimeler

• bor
• potasyum metaborat
• antioksidant
• antimikrobiyal
• antibiyofilm

In vitro biological activities of potassium metaborate; antioxidative, antimicrobial and antibiofilm properties

Öz

Antioxidant, antimicrobial and antibiofilm activities of potassium metaborate (KBO2) was investigated within the present study. Antioxidant capacity of potassium metaborate was determined by β-carotene bleaching (BCB) assay and hydroxyl radical scavenging activity. Potassium metaborate was evaluated for its antimicrobial effects against selected Gram-positive bacteria, Gram-negative bacteria and a yeast via broth dilution method. The inhibition capability of potassium metaborate on the microbial biofilm formation of tested microorganisms was measured by microplate biofilm method using MTT (3- [4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide). Biofilm inhibition capacity of potassium metaborate was also observed by Scanning Electron Microscope (SEM). Potassium metaborate was found to have the ability to scavenge hydroxyl radicals with an inhibition rate of 71.13% at 100 mM concentration. Antioxidant activity of potassium metaborate as determined by BCB assay gave higher result with an inhibition rate of 86.96% at the same concentration. According to the MIC (minimum inhibition concentration) values, the potassium metaborate inhibited the growth of C. albicans, S. aureus and E. coli at 62.5 mM concentrations while it was 31.25 mM for B. subtilis and 125 mM for P. aeruginosa. The highest antibiofilm activity was determined at the MIC of potassium metaborate with the reduction rate of 90.18% against C. albicans. It was concluded that, potassium metaborate have strong biological activities and can be effectively used for biomedical and environmental solutions.

Anahtar Kelimeler

• boron
• potassium metaborate
• antioxidant
• antimicrobial
• antibiofilm

Kaynakça

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