Liquidambar orientalis Miller in vitro kültürlerinde farklı bor tuzu uygulamalarının mikro çoğaltma ve genetik stabilite üzerine etkileri

Öz

Bu çalışmada, relikt endemik bir bitki türü olan Liquidambar orientalis'in mikroçoğaltımı üzerinde bor tuzlarının etkileri araştırılmış ve mikro sürgünlerin genetik stabilitesi ISSR markör tekniği ile belirlenmiştir. Tuz stresi, özellikle tuzluluk oranı ve kuraklığa toleransı düşük olan türlerde, bitki büyüme ve gelişmesi, strese yanıt olarak sekonder metabolit içeriğinde artış, somaklonal varyasyon gibi fizyolojik ve moleküler değişikliklere neden olabilir. Bu kapsamda, in vitro klonal çoğaltılmış L. orientalis'ten izole edilen meristemlere borik asit, sodyum perborat, sodyum metaborat ve disodyum oktaborat tuzlarının üç farklı konsantrasyonu uygulanmış ve bu bor tuzlarının meristem rejenerasyonu ve gelişimi üzerindeki etkileri değerlendirilmiştir. Tuz uygulaması yapılmayan kontrol grubu örnekleri ile karşılaştırıldığında %100 oranında en iyi rejenerasyon 1mgL-1 disodyum oktaborat uygulaması sonrası belirlenirken, sürgün oluşturma kapasite indeksi değerlendirildiğinde en iyi sonuç 4,94 ile 5mgL-1 sodyum perborat uygulamasından elde edilmiştir. Ancak anaç ile karşılaştırıldığında tüm tuz uygulamalarının genetik stabilite üzerinde somaklonal varyasyona neden olduğu görülmüş ve analiz edilen veriler ışığında en düşük %30 (5mgL-1 disodyum oktaborat) ve en yüksek %49 (1 mgL-1 borik asit) somaklonal varyasyon tüm uygulamalarda belirlenmiştir.

Anahtar Kelimeler

• Borik asit
• Disodyum oktaborat
• Sodyum metaborat
• Sodyum perborat
• ISSR belirteç

Effects of different boron salt treatments on micropropagation and genetic stability in in vitro cultures of Liquidambar orientalis Miller

Öz

In the present study, the boron salts effects on the micropropagation of Liquidambar orientalis, a relict-endemic plant species, were investigated and genetic stability of micro-shoots was determined by ISSR marker technique. Especially in species with low salinity and drought tolerance, salt stress may cause physiological and molecular changes such as plant growth and development, increase in secondary metabolite content in response to stress, and somaclonal variation. In this context, three different concentrations of boric acid, sodium perborate, sodium metaborate and disodium octaborate salts were applied to meristems isolated from in vitro clonal propagated L. orientalis and the effects of these boron salts on meristem regeneration and development were evaluated. When compared to the control group samples in which no salt application was applied, the best regeneration percentage was determined as 1mgL-1 disodium octaborate treatment with a value of 100%, while when the shoot forming capacity index was evaluated, 5mgL-1 sodium perborate treatment with a value of 4.94 gave the best results. However, when compared with the mother plant, it was observed that all salt treatments caused somaclonal variation on genetic stability, and in the light of the analyzed data, the lowest 30% (5mgL-1 disodium octaborate) and the highest 49% (1 mgL-1 boric acid) somaclonal variation were determined in all applications.

Anahtar Kelimeler

• Boric acid
• Disodium octaborate
• ISSR marker
• Sodium metaborate
• Sodium perborate

Kaynakça

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