Çinko borat’ın antimikrobiyal, antifibrinolitik, enzim inhibisyonu ve yara iyileşme özellikleri

Öz

Boron içerikli bileşenler son zamanlarda farmasötik uygulamalarda kullanılmaktadır. Esansiyel bir element olan Çinko (Zn) umut veren biyobozunur metallerden biridir. Bu çalışma, çinko borat’ın antimikrobiyal, antifibrinolitik, yara iyileşme ve enzim inhibisyonu karakteristiklerini belirlemek üzere tasarlanmıştır. In vitro yara çizik testine göre yara oluşumun 24. saatinde çinko boratın 0.01 µg/mL konsantrasyonda 3T3 fibroblast hücrelerinin proliferasyonunu uyardığı belirlenmiştir. En yüksek enzim inhibisyonu kollagenaza karşı 1 mg/mL konsantrasyonda gözlenmiştir. Candida albicans ve Staphylococcus aureus’a karşı çinko boratın minimum inhibisyon konsantrasyonları (MIC) sırasıyla 1 mg/mL ve 0.5 mg/mL olarak tespit edilmiştir. Çinko boratın 1, 0.5 ve 0.1 mg/mL konsantrasyonlarda herhangi bir fibrinolitik aktivitesi gözlenmemiştir. Çinko boratın yara iyileşme sürecini iyileştirmede etkin bir şekilde kullanılabileceği ve muhtemel yara enfeksiyonlarını önleyebileceği sonucuna varılmıştır.

Anahtar Kelimeler

• zinc borate
• wound healing
• enzyme inhibition
• antimicrobial
• antifibrinolytic

Antimicrobial, antifibrinolytic, enzyme inhibitory and wound healing properties of zinc borate

Öz

Boron containing compounds (BCGs) have recently been used for pharmaceutical applications. Zinc, an essential element, is known to be one of the most promising biodegradable metals. The present study was conducted to determine the wound healing properties of zinc borate with its antimicrobial, antifibrinolytic and enzyme inhibitory characteristics. In vitro scratch wound healing assay revealed that zinc borate at 0.01 µg/mL concentration stimulated the proliferation of 3T3 fibroblast cells after 24 h of scar formation. The highest enzyme inhibition was observed against collagenase at 1 mg/mL (81.5%). Minimum inhibition concentration (MIC) values were determined as 1 mg/mL and 0.5 mg/mL against Candida albicans and Staphylococcus aureus, respectively. Zinc borate did not have any fibrinolytic activity at 1, 0.5 and 0.1 mg/mL concentrations. It can be suggested that zinc borate can be used effectively to improve the wound healing process and to prevent the possible wound infections.

Anahtar Kelimeler

• zinc borate
• wound healing
• enzyme inhibition
• antimicrobial
• antifibrinolytic

Kaynakça

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