Topikal Uygulamalar için Antimikrobiyal Kitosan-Sodyum tetrafloroborat (NaBF4) Hidrojeller

Öz

Bir yaranın enfeksiyonu, yaralanmış bir dokunun iyileşmesini geciktiren en önemli sebeplerden biridir. Bu çalışmada, kitosan bazlı hidrojeller, antimikrobiyal bir yara bakım ürünü geliştirmek için farklı konsantrasyonlarda sodyum tetrafloroborat (NaBF4) ile yüklenmiştir. NaBF4'ün antimikrobiyal aktivitesi ve sitotoksisitesi ve Kitosan:NaBF4 hidrojellerinin antibiyotiğe dirençli bir tür de dahil olmak üzere geniş bir mikroorganizma spektrumuna karşı antimikrobiyal aktivitesi, yüzey morfolojisi ve kimyasal bağ yapıları incelenmiştir. NaBF4, gram-pozitif bakteriler için gram-negatif bakterilerden daha yüksek antibakteriyel aktivite göstermiştir. NaBF4'ün MİK değerleri gram negatif, gram pozitif ve mantar türülerinin tamamı için aynı olmak koşuluyla sırasıyla 3,906, 1,953 ve 7,813 µg/µL olarak bulunmuştur. NaBF4'ün L929 hücre hattı üzerindeki doğrudan sitotoksisitesi, MTT ile araştırılmıştır. 24 saatlik inkübasyon sonrası IC50 değeri 3,2 µg/µL olarak hesaplanmış ve antimikrobiyal aktiviteye sahip konsantrasyon aralığında yer aldığı belirlenmiştir. Kitosan hidrojellerinin antimikrobiyal aktiviteleri disk difüzyon yöntemi ile araştırılmıştır. Hidrojelin antimikrobiyal aktivitesi artan NaBF4 konsantrasyonu ile artarken, tek başına yüksek moleküler ağırlıklı kitosandan üretilen hidrojellerde antimikrobiyal aktivite gözlemlenmemiştir. Sonuçlara göre, geniş spektrumlu antimikrobiyal aktivite elde etmek için 1:3 (hidrojeldeki NaBF4 konsantrasyonu 546,5mM) örneğinin yeterli olduğu ve bu formülasyonla hazırlanan hidrojellerin potansiyel bir antimikrobiyal yara bakım ürünü olarak kullanılabileceği öngörülmüştür.

Anahtar Kelimeler

• Chitosan
• sodium tetrafluoroborate
• antimicrobial hydrogel
• cytotoxicity

Antimicrobial chitosan-sodium tetrafluoroborate (NaBF4) hydrogels for topical applications

Öz

Infection of a wound is one of the most important reasons delaying the recovery of an injured tissue. In this study, chitosan-based hydrogels were loaded with different concentrations of sodium tetrafluoroborate (NaBF4) to fabricate an antimicrobial wound care system. Antimicrobial activity, and cytotoxicity of NaBF4, and surface morphology, chemical bond structures and antimicrobial activity of Chitosan:NaBF4 hydrogels against a broad spectrum of microorganisms including an antibiotic resistant species were investigated. NaBF4 showed higher antibacterial activity for gram-positive bacteria than gram-negative bacteria. MIC values of NaBF4 were 3.906, 1.953, and 7.813 µg/µL for every gram-negative, gram-positive, and fungal species, respectively. Direct cytotoxicity of NaBF4 on the L929 cell line was investigated by the MTT assay. IC50 value after 24 h incubation was calculated as 3.2 µg/µL which is within the range of concentration with antimicrobial activity. The antimicrobial activities of chitosan hydrogels were investigated by disc diffusion method. Antimicrobial activity of hydrogel increased with increasing NaBF4 concentration while parent high molecular weight chitosan-based hydrogel did not show antimicrobial activity. According to the results, sample 1:3 (NaBF4 concertation is 546.5mM in the hydrogel) was enough to achieve broad spectrum antimicrobial activity and hydrogels prepared with this formulation can be used as a potential antimicrobial wound care product.

Anahtar Kelimeler

• Chitosan
• sodium tetrafluoroborate
• antimicrobial hydrogel
• cytotoxicity

Kaynakça

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