Betacoronavirus ve S. aureus'un hekzagonal bor nitrür ile etkileşimi

Öz

Nanopartikül ve boron ihtiva eden bileşikler üzerine elde edilen son gelişmeler, son yıllarda daha fazla ilgi gösterilmesine yol açmıştır. Bu çalışmada bor nitrür nanopartiküllerinin Bovine Coronavirus (BCoV) ve Staphylococcus aureus (S. aureus) üzerine etkisi araştırılmıştır. Deneylerden önce 0,5 mg/mL'den düşük konsantrasyonlardaki BNNP’nin HRT-18 hücre kültüründeki etkileri 5 gün süreyle mikroskobik ve hücre canlık testiyle incelenmiştir.takiben hBN, önceden BCoV ekimi yapılmış hücre kültür sıvısına da eklenmiştir. Ayrıca farklı konsantrasyonlardaki ürün hBN, sıvı ortamda ve kaplanmış membrane yüzeyinde BCoV ile biraraya getirilerek virus replikasyon ya da virusun enfektivite gücündeki etkisi araştırılmıştır. Diğer yandan spreyleme yöntemi ile hBN kaplanmış membranda Staphylococcus aureus (S. aureus) kullanılarak tıbbi Bakteriyel Filtrasyon Verimliliğini (BFE) değerlendirilmiştir. HRT-18 hücre hattında belirgin bir morfolojik değişiklik saptanmasa da canlı hücre sayımı yöntemi ile hBN hafif toksik olarak değerlendirilmiştir. BCoV etkileşimini incelendiği 0,3 mg/mL ve altındaki konsantrasyonlarda (0,025 or 0,3 mg/mL) hBNN uygulamasının virus süspansiyonunda enfeksiyözite gücünde azalmaya yol açmadığı, aynı zamanda in-vitro koşullarda virus replikasyonu üzerine inhibitör etkide olmadığı görüldü. Bakteriyel Filtrasyon Verimliliği testinde ise hBN kaplanmış polipropilen kumaşın %90,3 ≥ oranında bariyer oluşturduğu, buna karşı hBN kaplanmamış kumaşın %64,6 ≥ oranıda bariyer oluşturduğu belirlenmiştir. Mevcut çalışma, BNNP'lerin tek başına BCoV'ler için dezenfektan veya ilaç için iyi bir aday olmadığını, ancak özellikle S. aureus gibi kolay sanitasyona ihtiyaç duyulan alanlarda kullanılmasının değerli olabileceğini göstermektedir.

Anahtar Kelimeler

• Bovine Coronavirus
• hücre canlılık testi
• hekzagonal bor nitrür
• in-vitro analiz
• S. aureus

Interaction of betacoronavirus and S. aureus with boron nitride nanoparticles (BNNPs)

Öz

Investigations on advance effects of boron-containing compounds have gained attention the last decade. This study was carried out to investigate the effect of hexagonal boron nitride nanoparticles (BNNPs) on Bovine Coronavirus (BCoV) and Staphylococcus aureus (S. aureus) by different methods. First, biological effects of different BNNPs concentrations lower than 0.5 mg/mL examined on HRT-18 (Human Rectal Tumor) for 5 days. Different concentrations of hBN mixed with BCoV in liquid, on membrane or directly on cells and examined for differences of titers or replications. And also Bacterial Filtration Efficiency (BFE) test of hBN powders coated on polypropylene fabric by spray method was applied against S. aureus. The compound was found slightly toxic on the HRT-18 cell line by live cell counting, while no remarkable morphological difference was observed. BNNPs treatment with 0.025 or 0.3mg/mL concentrations did not reduce the infective titer and create no inhibitory effect on in vitro replication. Stability of virus titer after treatment of BNNPs coated fabric also indicated no antiviral efficiency. But hBN applied fabric formed a barrier of ≥90.3%, while non hBN applied fabric formed ≥64.6% barrier. The present study demonstrate that, BNNPs alone is not a good candidate for disinfectant or drug for BCoVs, while it could be valuable to use as coated fabric in the areas needing easy sanitation especially for S. aureus.

Anahtar Kelimeler

• Bovine Coronavirus
• cell viability
• hexagonal boron nitride
• in-vitro analysis
• S. aureus

Kaynakça

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