Kemik doku mühendisliği için kalsiyum fruktoboratın bölgesel salımına yönelik PCL/PVA/PCL taşıyıcı geliştirilmesi

Öz

Kalsiyum fruktoborat (CaFB), bor ve kalsiyum içeriğinin kemik sağlığını, oluşumunu ve yenilenmesini desteklediği bilindiği için dikkat çekmektedir. Önceki çalışmalar CaFB'nin kemik sağlığı üzerinde olumlu etkisi olduğunu ve kemik benzeri özellikleri desteklediğini kanıtlamıştır. Bu bilgilerin ışığında, bölgesel CaFB salımı yapan hücre taşıyıcı, kemik dokusu kaybı durumunda kemik yenilenmesini iyileştirecektir. Bu çalışma, kemik dokusu iyileşmesini teşvik etmek için CaFB'nin kontrollü lokal salımını yapabilen, katman katman polimerik bir sünger tasarlamayı amaçlamaktadır. CaFB'nin Saos-2 hücre hattının hücre canlılığı üzerindeki doza bağlı etkisi in vitro olarak araştırılmıştır. Polimerik taşıyıcının katman katman yapısı, 7 günlük inkübasyonun ardından %33,9±7,4 salımı gerçekleşen CaFB'nin kontrollü salımını desteklemiştir. 31,25 µg/mL konsantrasyonundaki CaFB, 1 ve 4 günlük inkübasyondan sonra Saos-2 hücre canlılığını %174,7±24,1 ve %127,7±8,7'ye kadar iyileştirmeyi başarmıştır. 7 günlük inkübasyondan sonra 250, 125, 62,5 ve 31,25 µg/mL konsantrasyondaki CaFB dozu hücre canlılığını %194,3±47,7, 155,3±17,7, 149,4±5,4 ve 132,5±13,3'e kadar arttırmıştır. PCL/PVA/PCL taşıyıcı hücrelerin canlılığını 7 gün boyunca desteklemiş ve taşıyıcının biyouyumlu olduğu gösterilmiştir. Bu çalışmanın sonuçları, CaFB'nin kemik doku rejenerasyonunu iyileştirmek için bir taşıyıcı sistemi içerisinde lokal olarak uygulanabilecek potansiyel bir bileşik olduğunu göstermiştir.

Anahtar Kelimeler

• Bor
• Kalsiyum Fruktoborat
• Kemik Doku Mühendisliği
• polikaprolakton
• polivinil alkol

Development of PCL/PVA/PCL scaffold for local delivery of calcium fructoborate for bone tissue engineering

Öz

Calcium fructoborate (CaFB) has gathered attention due to its boron and calcium content, both of which are known to support bone health, deposition and regeneration. Previous studies have shown that CaFB has a positive effect on bone health and has been proven to promote bone-like properties. In light of this information, a local CaFB delivering scaffold could improve bone regeneration in cases of bone tissue loss. This study aimed to design a layer-by-layer polymeric sponge capable of achieving controlled local delivery of CaFB to improve bone tissue healing. The dose-dependent effect of CaFB on the cell viability of the Saos-2 cell line was investigated in vitro. Layer by-layer structure of the polymeric scaffold supported controlled release of CaFB, with 33.9±7.4% released after 7 days of incubation. CaFB at 31.25 μg/mL concentration was able to improve Saos-2 cell viability up to 174.7±24.1% and 127.7±8.7% after 1 and 4 days of incubation. After 7 days of incubation CaFB treatment at concentrations of 250, 125, 62.5 and 31.25 μg/mL improved cell viability up to 194.3±47.7, 155.3±17.7, 149.4±5.4 and 132.5±13.3%. The polycaprolactone/polyvinyl alcohol/polycaprolactonen(PCL/PVA/PCL) scaffold supported the viability of cells for 7 days and was shown to be biocompatible. The results of this study showed that CaFB is a potential compound thatncan be locally delivered within a scaffold system to improve bone tissue regeneration.

Anahtar Kelimeler

• Bone tissue engineering
• Boron
• Calcium fructoborate
• Polycaprolactone
• Polyvinyl alcohol

Kaynakça

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