The antiproliferative and antimigrative effects of disodium octaborate tetrahydrate on A549 lung and DU 145 prostate cancer cells

Öz

Disodium octaborate tetrahydrate (DOT) (Na2O.4B2O3.4H2O) is an inorganic salt form of boron, used in agriculture, insecticides, and antifungal in wood protection. As an important fine boride, DOT is non-toxic for humans. The antimicrobial and wound-healing properties, as well as the cytotoxicity of DOT against glioblastoma cells, were investigated in other studies. Although there are no studies about the cytotoxic potential of other cancer types of this compound. This is the first study to investigate the cytotoxic and wound closure inhibition effects of DOT on A549 lung and DU145 prostate cancer cell lines. Cytotoxicity was assessed using the MTT assay, and the IC₅₀ values for A549 and DU145 cells were calculated as 58.78 mM and 109.33 mM, respectively, indicating a dose-dependent reduction in cell viability. The effect on wound closure was evaluated using an in vitro scratch assay. At IC₅₀ concentrations, DOT significantly inhibited wound closure in both cell lines after 24 hours compared to controls. Although the relatively high IC₅₀ values limit direct therapeutic applicability, these preliminary findings demonstrate, for the first time, that DOT possesses biological activity against lung and prostate cancer cells. Further studies are warranted to explore its potential applications.

Anahtar Kelimeler

• Boron
• A549
• DU 145
• Cyotoxicity
• Wound closure inhibition

Destekleyen Kurum

A part of this publication is a part of a project supported by the Muğla Sıtkı Koçman University Scientific Research Projects Coordination Unit under project number 21/123/02/1/4.

Etik Beyan

Ethics committee certification is not required.

Teşekkür

We thank to Muğla Sıtkı Koçman University Scientific Research Projects Coordination Unit for their support.

Disodyum oktaborat tetrahidratın A549 akciğer ve DU 145 prostat kanseri hücreleri üzerindeki antiproliferatif ve antimigratif etkileri

Öz

Disodyum oktaborat tetrahidrat (DOT) (Na2O.4B2O3.4H2O), tarımda, böcek ilaçlarında ve ahşap korumasında antifungal olarak kullanılan, borun inorganik bir tuz formudur. Önemli bir ince borür olan DOT, insanlar için toksik değildir. DOT'un antimikrobiyal ve yara iyileştirici özellikleri ile glioblastoma hücrelerine karşı sitotoksik etkisi diğer çalışmalarda araştırılmıştır. Bununla birlikte, bu bileşiğin diğer kanser türleri üzerindeki sitotoksik potansiyeli hakkında herhangi bir çalışma bulunmamaktadır. Bu çalışma, DOT'un A549 akciğer ve DU145 prostat kanseri hücre hatları üzerindeki antiproliferatif ve antimigratif etkilerini belirleyen ilk çalışmadır. DOT'un hücre göçü üzerindeki etkisi, in vitro çizik testi kullanılarak belirlenmiştir. A549 ve DU145 hücreleri için IC50 değerleri sırasıyla 59,55 mM ve 109,33 mM olarak tahmin edilmiştir. DOT'un A549 ve DU 145 hücreleri üzerindeki antiproliferatif etkisi doza bağlı olarak artmaktadır. İn vitro çizik testinde, DOT, kontrol grubuna kıyasla her iki hücre hattında da IC50 değerlerinde hücre göçünü inhibe etmiştir. Çalışma, DOT'un antiproliferatif ve antimigratuar etkileriyle kanser tedavisi için uygun bir aday olduğunu ortaya koymuştur. Bununla birlikte, klinik uygulamalarını desteklemek için daha in vivo çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

• Bor
• A549
• DU 145
• Sitotoksisite
• Yara kapanması inhibisyonu

Destekleyen Kurum

Bu yayının bir kısmı, Muğla Sıtkı Koçman Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 21/123/02/1/4 proje numarası kapsamında desteklenen projenin bir parçasıdır.

Etik Beyan

Etik kurul belgesi gerekmemektedir.

Teşekkür

Muğla Sıtkı Koçman Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'ne katkılarından dolayı teşekkür ederim.

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