Mycobacterium avium subsp. paratuberculosis ile Enfekte Sığırlarda Bazı Akut Faz Protein ve Biyokimyasal Parametre Düzeylerinin Belirlenmesi

Yıl 2022, Cilt: 3 Sayı: 2, 47 - 51, 30.12.2022

Kadir Bozukluhan Oğuz Merhan Fatih Büyük Enes Akyüz Tahir Gezer Hale Ergin Eğritağ Gürbüz Gökçe

Öz

Paratüberkülozis, kronik diyare ve granülomatöz enteritis ile karakterize evcil ve vahşi ruminantlarda görülen bulaşıcı bir hastalıktır. Çalışmanın amacı paratüberküloz ile enfekte sığırlarda bazı akut faz protein ve biyokimyasal parametre düzeylerinin belirlenmesidir. Çalışmada ELISA test sonuçlarına göre 15 paratüberküloz ile enfekte ve 15 antikor negatif sağlıklı sığır serumu kullanıldı. Serumlar haptoglobin, seruloplazmin, serum amiloid A (SAA), alkalen fosfataz (ALP), gama-glutamil transferaz (GGT), total protein, üre, kreatinin ve demir ölçümünde kullanıldı. Paratüberkülozisli sığırlar ile kontrol grubundaki hayvanlar karşılaştırıldığında haptoglobin, SAA (P<0.001), üre (P<0.05), seruloplazmin, kreatinin düzeylerinin (P<0.01) ve bunun yanı sıra ALP ve GGT aktivitesinin (P<0.01) arttığı, albümin ve total protein düzeyinin (P<0.01) ise azaldığı belirlendi. Demir düzeyi kontrol grubuna göre azalmakla beraber istatistiksel olarak anlamsız tespit edildi. Sonuç olarak, elde edilen bulguların paratüberkülozisin teşhisine katkı sağlayacağı, haptoglobin ve SAA’nın hastalığın teşhisinde yardımcı parametre olarak kullanılabilecekleri ve bu konuda daha detaylı çalışmaların yapılması gerektiği düşünülmektedir.

Anahtar Kelimeler

Sığır, Paratüberküloz, Akut faz protein, Biyokimyasal parametreler, Cattle, Paratuberculosis, Acute phase protein, Biochemical parameters

Determination of Some Acute Phase Protein and Biochemical Parameter Levels in Cattle Infected with Mycobacterium avium subsp. paratuberculosis

Öz

Paratuberculosis is an infectious disease in domestic and wild ruminants characterized by chronic diarrhea and granulomatous enteritis. The aim of the study is to determine some acute phase protein and biochemical parameter levels in cattle infected with paratuberculosis. In the study, 15 paratuberculosis-infected and 15 antibody-negative healthy bovine serums were used according to ELISA test results. The sera were used for the measurement of haptoglobin, ceruloplasmin, serum amyloid A (SAA), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total protein, urea, creatinine and iron. When the cattle with paratuberculosis and the animals in the control group were compared, it was determined that haptoglobin, SAA (P<0.001), urea (P<0.05), ceruloplasmin, creatinine levels (P<0.01) as well as ALP and GGT activities (P<0.01) increased, while albumin and total protein levels (P<0.01) decreased. Although the iron level decreased compared to the control group, it was found to be statistically insignificant. As a result, it is thought that the obtained findings will contribute to the diagnosis of paratuberculosis, haptoglobin and SAA can be used as auxiliary parameters in the diagnosis of the disease, and more detailed studies should be done on this subject.

Anahtar Kelimeler

Sığır, Paratüberküloz, Akut faz protein, Biyokimyasal parametreler, Cattle, Paratuberculosis, Acute phase protein, Biochemical parameters

Kaynakça

• 1. Baumgartner W and Khol JL. Paratuberculosis (Johne’s disease) in ruminants- an ongoing story. Slovenian Veterinary Research 2016; 43: 5-10.
• 2. Constable PD, Hinckliff KW, Done SH, Grunberg W. Veterinary Medicine. Eleventh Edition. London: W.B. Saunders Company, 2017; p.1591-1593.
• 3. Hasonova L and Pavlik I. Economic impact of paratuberculosis in dairy cattle herds: a review. Veterinarni Medicina 2006; 51: 193-211.
• 4. Civelek T. Süt sığırlarında paratüberküloz. Kocatepe Veterinary Journal 2017; 10: 29-32. doi: 10.5578/kvj.35300.
• 5. Osterstock JB, Sinha S, Seabury CM, Cohen ND. Effect of classifying disease states in genetic association studies for paratuberculosis. Preventive Veterinary Medicine 2010; 95: 41-49. doi: 10.1016/j.prevetmed.2010.03.005.
• 6. Mecitoğlu Z ve Demir G. Sığırlarda paratüberkülozun tanısına ilişkin problemler. Journal of Research in Veterinary Medicine 2012; 1: 19-23.
• 7. Tothova C, Nagy O, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Veterinarni Medicina 2014; 59: 163-180. doi: 10.17221/7478-VETMED.
• 8. Merhan O, Bozukluhan K. Acute phase response and some acute phase proteins in animals. Yıldız G, Baran MS, Kaplan O, Durna Aydın Ö. eds. In: Current Multidisciplinary Studies in Veterinary Medicine I. Ankara: Iksad Publishing House; 2022; pp. 3-33.
• 9. Petersen HH, Nielsen JP, Heegaard PMH. Application of acute phase protein measurements in veterinary clinical chemistry. Veterinary Research 2004; 35: 163-187. doi: 10.1051/vetres:2004002.
• 10. Bozukluhan K, Merhan O. Clinical significance of some acute phase proteins in cattle. Kükürt A, Gelen V. eds. In: Cattle Diseases-Molecular and Biochemical Approach. London: IntechOpen, 2022; pp.1-13.
• 11. Skinner JG, Brown RA, Roberts L. Bovine haptoglobin response in clinically defined field conditions. Veterinary Record 1991; 128: 147-149. doi: 10.1136/vr.128.7.147.
• 12. Colombo JP and Richterich R. Zur bestimmung des caeruloplasmin im plasma [on the determination of ceruloplasmin in plasma]. Schweizerische Medizinische Wochenschrift 1964; 94: 715-720.
• 13. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Veterinary Journal 2004; 168: 28-40. doi: 10.1016/S1090-0233(03)00119-9.
• 14. Ceciliani F, Ceron JJ, Eckersall PD, Sauerwein H. Acute phase proteins in ruminants. Journal of Proteomics 2012; 75: 4207-4231. doi: 10.1016/j.jprot.2012.04.004.
• 15. Merhan O, Bozukluhan K, Gokce HI. Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. Journal of the Hellenic Veterinary Medical Society 2017; 68: 535-540. doi: 10.12681/jhvms.16049.
• 16. Merhan O, Yılmaz V, Bozukluhan K, Maraşlı Ş, Öğün M. Investigation of some acute phase proteins and antioxidant/oxidant system in infected sheep with bluetongue virus disease. Journal of Advances in VetBio Science and Techniques 2022; 7: 259-264. doi: 10.31797/vetbio.1063406.
• 17. Merhan O, Bozukluhan K, Çelebi Ö, Öğün M, Atakişi E, et al. Levels of acute phase protein and some biochemical parameter in cattle infected with Mycobacterium bovis. Journal of the Faculty of Veterinary Medicine Erciyes University 2017; 14: 101-105.
• 18. Kaya S, Merhan O, Kacar C, Colak A, Bozukluhan K. Determination of ceruloplasmin, some other acute phase proteins, and biochemical parameters in cows with endometritis. Veterinary World 2016; 9: 1056-1062. doi: 10.14202/vetworld.2016.1056-1062.
• 19. Bozukluhan K, Merhan O, Özcan A, Gökçe Hİ, Gökçe G: Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi 2017; 64: 75-79. doi: 10.1501/Vetfak_0000002778.
• 20. Bozukluhan K, Merhan O, Kiziltepe S, Ergin Egritag H, Akyuz E, et al. Determination of haptoglobin, some biochemical and oxidative stress parameters in calves with pneumonia. Fresenius Environmental Bulletin 2021; 30: 9485-9489.
• 21. Kırbaş A, Kaman R, Merhan O, Bozukluhan K, Değirmençay S, et al. Evaluation of acute phase response and oxidative damage in sheep naturally infected with Streptococcus pluranimalium using haptoglobin, nitric oxide, and malondialdehyde levels. Fırat University Veterinary Journal of Health Sciences 2021; 35: 145-150.
• 22. Trela M, Domanska D, Witkowska-Piłaszewicz O. Diagnostic use of serum amyloid A in dairy cattle. Agriculture 2022; 12: 459. doi: 10.3390/agriculture1204045.
• 23. Bozukluhan K, Merhan O, Büyük F, Çelebi Ö, Gökçe G. Brusellozisli sığırlarda bazı akut faz proteinlerin düzeyinin saptanması. Ankara Üniversitesi Veteriner Fakültesi Dergisi 2016; 63: 13-16. doi: 10.1501/Vetfak_0000002703.
• 24. Bozukluhan K, Merhan O, Ogun M, Kurt B, Cihan M, et al. Investigation of haptoglobin, serum amyloid A, and some biochemical parameters in calves with omphalitis. Veterinary World 2018; 11: 1055-1058. doi: 10.14202/vetworld.2018.1055-1058.
• 25. Sevgisunar NM and Şahinduran Ş. Evaluation of some acute phase proteins, cytokines and hepcidin levels in naturally infected Saanen goats with paratuberculosis. MAKU Journal of Health Science Institute 2021; 9: 29-37. doi: 10.24998/maeusabed.996891.
• 26. Merhan O, Bozukluhan K, Kiziltepe S, Gokce HI. Investigation of levels of haptoglobin, serum amyloid A, ceruloplasmin and albumin in cattle with foot-and-mouth disease. Israel Journal of Veterinary Medicine 2017; 72: 14-17.
• 27. Bozukluhan K, Merhan, O, Gökçe Hİ, Öğün M, Atakişi E, et al. Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected sheeppox virus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2018; 24: 437-441. doi: 10.9775/kvfd.2017.19167.
• 28. Kırmızıgül AH, Erkılıç EE, Merhan O, Öğün M, Ölmez N, ve ark. Babesia canis ile enfekte köpeklerde serum amiloid A, haptoglobin, seruloplazmin ve albumin seviyeleri. Kocatepe Veteriner Dergisi 2020; 13: 219-223. doi: 10.30607/kvj.670744.
• 29. Bozukluhan K ve Gökçe Hİ. Retikuloperitonitis travmatika ve retikuloperikarditis travmatika’lı sığırlarda bazı akut faz proteinlerin araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 2007; 4: 107-113.
• 30. Cerone SI, Sansinanea AS, Streitenberger SA, Garcia MC, Auza NJ. Cytochrome c oxidase, Cu, Zn-superoxide dismutase, and ceruloplasmin activities in copper-deficient bovines. Biological Trace Element Research 2000; 73: 269-278. doi: 10.1385/BTER:73:3:269.
• 31. Duran U and Cenesiz S. Determination of acute phase protein levels in cattle with paratuberculosis. International Journal of Advances in Science, Engineering and Technology 2019; 46-49.
• 32. Espinosa J, de la Morena R, Benavides J, Garcia-Pariente C, Fernandez M, et al. Assessment of acute-phase protein response associated with the different pathological forms of bovine paratuberculosis. Animals (Basel) 2020 10:1925. doi: 10.3390/ani10101925.
• 33. Russell KE, Roussel AJ. Evaluation of the ruminant serum chemistry profile. The Veterinary Clinics of North America Food Animal Practice 2007; 23: 403-426. doi: 10.1016/j.cvfa.2007.07.003.
• 34. Gruys E, Toussaint MJM, Niewold TA, Koopmans SJ. Acute phase reactant and acute phase proteins. Journal of Zhejiang University Science B. 2005; 6: 1045-1056. doi: 10.1631/jzus.2005.B1045.
• 35. El-Deeb WM, Fouda TA, El-Bahr SM. Clinico-biochemical investigation of paratuberculosis of dromedary camels in Saudi Arabia: proinflammatory cytokines, acute phase proteins and oxidative stress biomarkers. Pakistan Veterinary Journal 2014; 34: 484-488.
• 36. Balikci E, Gurdogan F. Some biochemical parameters and oxidative stress biomarkers in sheep with paratuberculosis. Medycyna Weterynaryjna 2015; 71: 679-682.
• 37. Kızıl Ö ve Çeribaşı S. Paratüberkülozisli bir koyunda serum biyokimyası ve patolojik bulgular. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 2010; 24: 163-166.
• 38. Tennant BC, Center SA. Hepatic function. Kaneko JJ, Harvey JW, Bruss ML. eds. In: Clinical Biochemistry of Domestic Animals. New York: Academic Press, 2008; pp.379-412.
• 39. Gokce HI and Woldehiwet Z. The effects of Ehrlichia (Cytoecetes) phagochytophila on the clinical chemistry of sheep and goats. Journal of Veterinary Medicine. Series B 1999; 46: 93-103. doi: 10.1111/j.0931-1793.1999.00210.x.
• 40. Aral H. Laboratory in diagnosis and monitoring of systemic inflammatory response syndrome or sepsis. European Journal of Basic Medical Sciences 2015; 5: 10-20. doi: 10.15197/sabad.2.5.02.