Rotavirus ile Mono- ya da Ko-Enfekte Buzağılarda Bağırsak Geçirgenliğinin Bir Adım Öncesinde Serum Zonulin Seviyeleri ile Dışkı Skorlarının Yansımaları

Yıl 2025, Cilt: 6 Sayı: 1, 16 - 21, 30.06.2025

Deniz Alıç Ural

https://doi.org/10.58833/bozokvetsci.1642162

Öz

Rotavirus kaynaklı ishallerde artmış olan intestinal permeabilite olası mekanizmalardan birisi olarak gösterilmektedir. Buna karşın karşıt görüş ile insan ve fare modellemelerinde, rotavirusların oluşagelen ishalin seyri esnasında intestinal permabiliteyi etkilemediği ya da azalttığı da bahis edilmektedir. Bu çalışmada özel bir buzağı işletmesinde rotavirus ile mono-infekte 10 ishalli (3 erkek, 7 dişi; 1-17 günlük), rotavirus ile diğer etmenlerle ko-enfekte 8 buzağı (2-24 günlük, 4 dişi, 4 erkek) ve diğer 7 sağlıklı (1-18 günlük; 5 dişi, 2 erkek olmak üzere toplamda 25 buzağıda bağırsak geçirgenliğinin araştırılması amaçlandı. Dışkı kıvamı 3’lü puantaj sistemi dahilinde değerlendirildiğinde mono-enfekte grupta 1-3 arası, ko-enfekte grupta 2 ile 3 bandında değişim tespit edildi Gizli temizlik skorları açısından yapılan değerlendirmede mono-infekte grupta 1 ila 2, ko-infekte grupta 2 ila 3 arasında skorlar değişmekte idi. Serum zonulin seviyeleri (ng/mL) ortalama±standart hata gösterildiği üzere rotavirus ile mono-infekte buzağılarda 41.59±6.41, ko-infekte buzağılarda 61.61±12.42 olarak belirlenirken, kontrol grubuna) 19.25±6.41 göre anlamlı farklar istatistiksel olarak belirgin şekilde (p<0.05) görüldü. Sonuç olarak rotavirus ile gerek mono-infekte gerekse ko-infekte buzağılarda muhtemel intestinal permabilite artışına eşlik eden geçirgen bağırsak sendromunun varlığı gerek teşhise yönelik biyobelirteç havuzuna katkı sağlayabilir, gerekse sağaltım protokollerinde değişime neden olabilir.

Anahtar Kelimeler

buzağı , intestinal permeabilite , ishal , rotavirüs , zonulin

Reflection of Serum Zonulin Levels and Fecal Scores One Step Before Intestinal Permeability in Calves Mono- or Co-Infected with Rotavirus

Öz

Increased intestinal permeability in rotavirus-induced diarrhea is shown as one of the possible mechanisms. However, it is also claimed by the opposing view that rotaviruses do not affect or reduce intestinal permeability during the course of diarrhea in human and mouse models. In this study, it was aimed to investigate intestinal permeability in a total of 25 calves, 10 with diarrhea (3 males, 7 females; 1-17 days old) mono-infected with rotavirus, 8 calves (2-24 days old, 4 females, 4 males) co-infected with rotavirus and other agents and 7 other healthy (1-18 days old; 5 females, 2 males) in a private calf farm. When stool consistency was evaluated within the 3-point scoring system, changes were detected between 1-3 in the mono-infected group and between 2 and 3 in the co-infected group. In the evaluation made in terms of latent cleanliness scores, the scores varied between 1 and 2 in the mono-infected group and between 2 and 3 in the co-infected group. Serum zonulin levels (ng/mL) were 41.59±6.41 in rotavirus mono-infected calves and 41.59±6.41 in co-infected calves as shown in the mean±standard error. 61.61±12.42, while it was determined as 19.25±6.41 compared to the control group, statistically significant differences were observed (p<0.05). As a result, the presence of leaky gut syndrome accompanied by a possible increase in intestinal permeability in both mono-infected and co-infected calves with rotavirus may contribute to the biomarker pool for diagnosis and may cause changes in treatment protocols.

Anahtar Kelimeler

calf , diarrhea , intestinal permeability , rotavirus , zonulin

Kaynakça

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