Investigation of Resistance of Some Lettuce Varieties against Lettuce Bacterial Leaf Spot Disease Agent Xanthomonas hortorum pv. vitians and the Susceptibility of the Disease Agent to Copper

Yıl 2024, Cilt: 7 Sayı: 6, 1 - 2

Richard Lee Kolleh Hüseyin Basım

https://doi.org/10.47115/bsagriculture.1501121

Öz

Bacterial Leaf Spot (BLS), caused by Xanthomonas hortorum pv. vitians (Xhv), is a significant disease affecting lettuce cultivation and causing crop losses. In this study, eight lettuce varieties were tested against the most virulent strain (HBXcv1) of Xanthomonas hortorum pv. vitians using two inoculation methods: hand sprayer and needle syringe. The symptoms appearing on lettuce plants ten days after bacterial inoculation using the spraying method were evaluated. The varieties' tolerance levels were determined using the Duncan Multiple Range Test (DMRT), with Carmesi RZ Lolo being the most tolerant variety (DMRT mean of 0.25 at 5% significance level) and Cospirina being the most sensitive (DMRT mean of 216.55 at 5% significance level). After bacterial inoculations using a syringe with a needle, similar reactions were observed in lettuce as those obtained from spray inoculations. Among different lettuce varieties, Carmesi RZ Lolo showed the most tolerance to the effect of HBXcv1. No symptoms were found in lettuce leaves after 10 days of inoculation. On the other hand, Cospirina, Nun 06118, and Presidential lettuce varieties were determined to be sensitive to HBXcv1. In the second part of the study, the sensitivity level of Xhv HBXcv1 to different copper sulfate concentrations was investigated using Nutrient Agar and Casitone Yeast extract agar. HBXcv1 strain was found to be resistant to 50ppm CuSO4 and sensitive to 100ppm and 200ppm. These findings contribute to understanding the response of lettuce cultivars to BLS. It has been found that copper-containing mixtures may be effective in the chemical control of Xhv infections.

Anahtar Kelimeler

Lettuce, Resistance, Sensitivity, Xanthomonas hortorum pv. vitians, Copper Sulphate

Etik Beyan

Ethics committee approval was not required for this study because of there was no study on animals or humans.

Destekleyen Kurum

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Proje Numarası

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Teşekkür

The authors give thanks to Prof. Dr. Esin BASIM (Akdeniz University, Antalya, Türkiye) for kindly providing assistance in laboratory work carried out in this research.

Kaynakça

• Al-Saleh M, Ibrahim Y. 2009. First report of bacterial leaf spot of lettuce (Lactuca sativa) caused by Xanthomonas campestris pv. vitians in Saudi Arabia. Int J Appl Plant Pathol, 93 (1): 107-107.
• Bari R, Jones JD. 2009. Role of plant hormones in plant defense responses. Plant Mol Biol, 69: 473-488.