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Evaluation of Associative Effects of Gliricidia sepium and Megathyrsus maximus Combinations on Fibre Fractions, In-Vitro Carbon Dioxide and Methane Production

Yıl 2025, Cilt: 8 Sayı: 3, 295 - 303, 15.05.2025
https://doi.org/10.47115/bsagriculture.1564072

Öz

The voluntary feed intake and digestibility of forages are contingent upon the quality of the pasture, which in turn has an impact on ruminant productivity. There is limited research on the proximate/chemical composition, in-vitro gas production, and degradability of mixtures containing Megathyrsus maximus (MM) and Gliricidia sepium (GS). The study investigated the impact of different combinations of MM and GS on in-vitro gas production, degradability, and chemical composition. Megathyrsus maximus (MM) and Gliricidia sepium (GS) were combined as follows: T1- 100%MM + 0%GS, T2- 75%MM + 25%GS, T3- 50%MM + 50%GS, T4- 25%MM + 75%GS, and T5- 0%MM + 100%GS. The treatments were assayed using standard procedures. The proximate composition of the treatments was also determined using standard procedures. Data were analyzed using descriptive statistics and ANOVA at α0.05. Initial gas produced in T1 and T2 and net gas volume (NGV) observed in T1 and T2 were significantly lower compared to other treatments. In addition, the CO2 gas produced in T3, T4, and T5 was significantly higher than that produced in T1 and T2. However, T1 had the lowest CO2 gas production. The treatment with 100% legume (T5) had the highest methane (CH4) production, followed by T4 and T3. Treatments containing 100% (T1) and 75% (T2) grass had the lowest CH4 production. In terms of degradability, it was observed that T5 had significantly higher organic matter degradability (OMD) compared to other dietary treatments. The crude protein observed in T5 was also significantly higher than other treatments. In conclusion, mixtures with a high content of soluble carbohydrates presented the lowest gas production. It was determined that a mixture of 75% Megathyrsus maximus and 25% Gliricidia sepium has increased carbohydrate, ash, lower moisture content, and in vitro gas production and can be utilized by ruminant farmers as a cheap and readily available source of nutrition for their animals.

Kaynakça

  • Aderinola OA, Lateef OA, Binuomote RT, Adeeyo A, Jekayinfa OA. 2014. Nutritional and microbial contents of varied combinations of ensiled Megathyrsus maximus and Vetiveria nigritana grass. Intr J Food Agri Vet Sci 4(1): 141- 148
  • Ajayi FT, Babayemi OJ. 2008. Comparative evaluation of mixtures of Panicum maximum cv Ntchisi with stylo (Stylosanthes guianensis), lablab (Lablab purpureus), centro (Centrosema pubescens) and tridax. Livestock Research for Rural Development, 20(6): 54.
  • Anim-Jnr AS, Sasu P, Bosch C, Mabiki FP, Frimpong YO, Emmambux MN, Greathead HMR. 2023. Sustainable small ruminant production in low- and middle-income African Countries: Harnessing the potential of agroecology. Sustainability, 15(21): 15326.
  • AOAC (Association of Official Analytical Chemists) 1990. Official methods of analysis. 15th Ed. Washington DC, USA, pp: 25.
  • AOAC, 2005. Official methods of analysis. Assoc Offic Analy Chem Washington, DC, USA, pp: 48.
  • Arango J, Ruden A, Martinez-Baron D, Loboguerrero AM, Berndt A, Chacón M, et al. 2020. Ambition meets reality: achieving GHG emission reduction targets in the livestock sector of Latin America Front Sustain Food Syst, 4: 65.
  • Babayemi OJ, Bamikole MA. 2006. Effect of Tephrosia candida DC leaf and its mixtures with guinea grass on the in vitro fermentation changes in feed for ruminants in Nigeria. Pakistan J Nutr 5(1): 14-18.
  • Babayemi OJ, 2007. In vitro fermentation characteristic and acceptability by West African dwarf goats of some dry season forages. African J Bio, 6(10): 1260-1265.
  • Bamikole MA, Babayemi OJ. 2004. Feeding goats with Guinea grass, veruno stylo and nitrogen fertilized grass with energy concentrate, Archi Zoot, 53: 13-23.
  • Barnes RF, Baylor JE. 1995. Forages in a changing world. In: Barnes RF, Miller DA, Nelson CJ. (eds) Forages, Vol. 1. An introduction to grassland agriculture. Iowa State University Press, Ames, Iowa, USA, pp: 3-13.
  • Bawala TO, Isah OA, Akinsoyinu AO. 2006. Studies on milk mineral composition of lactating West African Dwarf goats. J Anim Vet advances 5(10): 805-809.
  • Beauchemin KA, McGinn SM, Benchaar C, Holtshausen L. 2009. Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: effects on methane production, rumen fermentation, and milk production. J Dairy Sci, 92(5): 2118-2127.
  • Boadi D, Benchaar C, Chiquette J, Masse D. 2004. Mitigation strategies to reduce enteric methane emissions from dairy cows: Update review. Canadian J Anim Scie, 84: 319-335.
  • Bonhomme A. 1990. Rumen ciliates: Their metabolism and relationships with bacteria and their hosts. Anim Feed Sci Tech, 30: 203-266.
  • Carulla J, Kreuzer M, Machmueller A, Hess H. 2005. Supplementation of acacia mearnsii tannins decrease methanogenesis and urinary nitrogen in forage-fed sheep. Australian J Agri Research, 56: 5022.
  • Chynoweth DP. 1996. Environmental impact of biomethanogenesis. Envi Moni Asses, 42: 3-18.
  • Cook BG, Pengelly BC, Schultze-Kraft R, Taylor M, Burkart S, Cardoso Arango JA, et al. 2020. Tropical forages: An interactive selection tool, 2nd and revised Edn. Cali; Nairobi: International center for tropical agriculture (CIAT); Colombia and international livestock research institute (ILRI). Available online at: www.tropicalforages.info (accessed date: January 15, 2025).
  • Demeyer D, De Meulemeester M, De Graeve K, Gupta BW. 1988. Effect of fungal treatment on nutritivevalue of straw. Med Fac Landbouww Rijks univ Gent 53: 1811-1819.
  • Dhiman TR, Satter LD. 1997. Yield response of dairy cows fed different proportions of alfafa silage and corn silage. J Dairy Sci, 80: 2069-2082.
  • Fievez V, Babayemi OJ, Demeyer D. 2005. Estimation of direct and indirect gas production in syringes: a tool to estimate short chain fatty acid production requiring minimal laboratory facilities. Anim Feed Sci Tech, 128-124, 197-210.
  • Forage and Grazing Terminology Committee 1991. Terminology for grazing Lands and grazing animals. Pocahontas Press, Blacksburg, Virginia, USA, pp: 45-60.
  • Getachew G, Makkar HPS, Becker K. 1999. Stochiometric relationship between short chain fatty acid and in vitro production in presence and absence of polyethylene glycol for tannin containing browses. In: FAAP satellite symposium gas production. Fermentation Kinetics for feed evaluation and to assess microbial activity. August 18-19 Wageningen, Netherlands, pp: 78.
  • Houghton J. 1997. Global warming. Geol Magaz, 135(6): 819-842.
  • Intergovernmental Panel on Climate Change (IPCC). 2013. The physical science basis. In Working group i contribution to the fifth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge, UK, pp: 54.
  • Janssen PH. 2010. Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics, Anim Feed Scie Tech, 160(12): 1-22.
  • Johnson KA, Johnson DE. 1995. Methane emissions from cattle. J Anim Sci, 73: 2483-2492.
  • Jouany JP. 1994. Les fermentations dans le rumen et leur optimisation. INRA Prod Anim 7(3): 207-225.
  • Kearny CC. 2005. Effects of dietary physical form and carbohydrate profile on captive giraffe. MSc Thesis, University of Florida, Gainesville, Florida, USA, pp: 87.
  • Leng RA. 1993. Quantitative ruminant nutrition-a green science. Australian J Agri Research, 44(3): 363-380.
  • Man NV, Wiktorsson H. 2003. Forage yield, nutritive value, feed intake, and digestibility of three grass species as affected by harvest frequency. Trop Grassland, 37: 101-110.
  • Mbah CN, Mbah EC, Orji JE, Igberi C, Abam P, Awere SU. 2024. Using Gliricidia sepium prunings as green manure in a degraded ultisol; effects on soil physical properties and yield of okra (Abelmuschus esculentus) in Abakaliki, southeast Nigeria. Biol Agri Hort, 40(4): 257-266.
  • McAllister TA, Cheng KJ, Okine EK, Mathison GW. 1996. Dietary, environmental, and microbiological aspects of methane production in ruminants. Canadian J Anim Sci 76(2): 231-243.
  • Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor. J Agri Sci, 93: 217.
  • Menke KH. Steingass. 1988. Estimation of the energy of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev, 28: 1-7.
  • Merry RJ, Lee MRF, Davies DR, Dewhurst RJ, Moorby JM, Scollan ND, Theodorou MK. 2006. Effects of high- sugar ryegrass silage and mixtures with red clover silage on ruminant digestion. I. In vitro and in vivo studies of nitrogen utilization. J Anim Sci, 84: 3049-3060.
  • Merten DR. 1997. Creating a system for meeting the fibre requirement of Dairy cattle. J Dairy Sci, 80: 1463-1482.
  • Moe PW, Tyrrell HF. 1979. Methane production in dairy cows. J Dairy Sci, 62(10): 1583-1586.
  • Norton BW. 2010. The Nutritive value of tree legumes, http://www.fao.org/ag/AGP/doc/Publication/Guttshel/×5556e0.html (accessed date: January 15, 2025).
  • Oba M, Allen MS. 1999. Evaluation of the importance of the digestibility of neutral detergent fibre from forage: Effects on dry matter intake and milk yield of dairy cows. J Dairy Sci, 82: 529-596.
  • Odeyinka SM. 2001. Effect of feeding varying levels of Leucaena leucocephala and Gliricidia sepium on the performance of West African Dwarf goats. Nigerian. J Anim Prod, 28(1): 61-64.
  • Orskov ER, McDonald I. 1979. The estimation of protein degradability in rumen from incubation measurements weighted according to the rate of passage. J Agri Sci Cambridge, 92: 449-503.
  • Preston TR. 1995. Tropical animal feeding. A manual for research workers. FAO Anim Prod Heal Paper 126, pp: 305.
  • Qin WZ, Li CY, Kim JK, Ju JG, Song MK. 2012. Effects of defaunation on fermentation characteristics and methane production by rumen microbes in vitro when incubated with starchy feed sources. Asian-Australasian J Anim Scie, 25(10): 1381-8. http://doi:10.5713/ajas.2012.12240.
  • Righi F, Ruini P, Romanelli S, Renzi M, Rossi F, Quarantelli A. 2008. Study of alternative field systems for the evaluation of total mixed ration physical form. Corso di Vet med - Università degli Studi di Parma Vol. 28: pp: 181- 190.
  • Shibata M. 1992. Methane production in heifers, sheep and goats consuming diets of various hay-concentrate ratios. J Anim Sci Tech, 63: 1221-1227.
  • Statistical Analysis System Institute Inc 1990 SAS/STAT® user’s guide Int Volume 1, version 6, Fourth Edition, Cary, NC, USA.
  • Swan CG, Bowman JGP, Martin JM, Giroux MJ. 2006. Increased puroindoline levels slow ruminal digestion of wheat (Triticum aestivum L.) starch by cattle. J Anim Sci Tech. 84: 641-650.
  • Theodorou MK, Williams BA, Dhanoa MS, McAllen AB, France J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci Tech, 48: 185-197.
  • Van Soest PJ, Robertson JB, Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci, 74(10): 3583-97.
  • Zhang J, Shi H, Wang Y, Li S, Cao Z, Ji S, He Y, Zhang H. 2017. Effect of dietary forage to concentrate ratios on dynamic profile changes and interactions of ruminal microbiota and metabolites in holstein heifers. Fronti in Microb. 8: 2206.

Evaluation of Associative Effects of Gliricidia sepium and Megathyrsus maximus Combinations on Fibre Fractions, In-Vitro Carbon Dioxide and Methane Production

Yıl 2025, Cilt: 8 Sayı: 3, 295 - 303, 15.05.2025
https://doi.org/10.47115/bsagriculture.1564072

Öz

The voluntary feed intake and digestibility of forages are contingent upon the quality of the pasture, which in turn has an impact on ruminant productivity. There is limited research on the proximate/chemical composition, in-vitro gas production, and degradability of mixtures containing Megathyrsus maximus (MM) and Gliricidia sepium (GS). The study investigated the impact of different combinations of MM and GS on in-vitro gas production, degradability, and chemical composition. Megathyrsus maximus (MM) and Gliricidia sepium (GS) were combined as follows: T1- 100%MM + 0%GS, T2- 75%MM + 25%GS, T3- 50%MM + 50%GS, T4- 25%MM + 75%GS, and T5- 0%MM + 100%GS. The treatments were assayed using standard procedures. The proximate composition of the treatments was also determined using standard procedures. Data were analyzed using descriptive statistics and ANOVA at α0.05. Initial gas produced in T1 and T2 and net gas volume (NGV) observed in T1 and T2 were significantly lower compared to other treatments. In addition, the CO2 gas produced in T3, T4, and T5 was significantly higher than that produced in T1 and T2. However, T1 had the lowest CO2 gas production. The treatment with 100% legume (T5) had the highest methane (CH4) production, followed by T4 and T3. Treatments containing 100% (T1) and 75% (T2) grass had the lowest CH4 production. In terms of degradability, it was observed that T5 had significantly higher organic matter degradability (OMD) compared to other dietary treatments. The crude protein observed in T5 was also significantly higher than other treatments. In conclusion, mixtures with a high content of soluble carbohydrates presented the lowest gas production. It was determined that a mixture of 75% Megathyrsus maximus and 25% Gliricidia sepium has increased carbohydrate, ash, lower moisture content, and in vitro gas production and can be utilized by ruminant farmers as a cheap and readily available source of nutrition for their animals.

Kaynakça

  • Aderinola OA, Lateef OA, Binuomote RT, Adeeyo A, Jekayinfa OA. 2014. Nutritional and microbial contents of varied combinations of ensiled Megathyrsus maximus and Vetiveria nigritana grass. Intr J Food Agri Vet Sci 4(1): 141- 148
  • Ajayi FT, Babayemi OJ. 2008. Comparative evaluation of mixtures of Panicum maximum cv Ntchisi with stylo (Stylosanthes guianensis), lablab (Lablab purpureus), centro (Centrosema pubescens) and tridax. Livestock Research for Rural Development, 20(6): 54.
  • Anim-Jnr AS, Sasu P, Bosch C, Mabiki FP, Frimpong YO, Emmambux MN, Greathead HMR. 2023. Sustainable small ruminant production in low- and middle-income African Countries: Harnessing the potential of agroecology. Sustainability, 15(21): 15326.
  • AOAC (Association of Official Analytical Chemists) 1990. Official methods of analysis. 15th Ed. Washington DC, USA, pp: 25.
  • AOAC, 2005. Official methods of analysis. Assoc Offic Analy Chem Washington, DC, USA, pp: 48.
  • Arango J, Ruden A, Martinez-Baron D, Loboguerrero AM, Berndt A, Chacón M, et al. 2020. Ambition meets reality: achieving GHG emission reduction targets in the livestock sector of Latin America Front Sustain Food Syst, 4: 65.
  • Babayemi OJ, Bamikole MA. 2006. Effect of Tephrosia candida DC leaf and its mixtures with guinea grass on the in vitro fermentation changes in feed for ruminants in Nigeria. Pakistan J Nutr 5(1): 14-18.
  • Babayemi OJ, 2007. In vitro fermentation characteristic and acceptability by West African dwarf goats of some dry season forages. African J Bio, 6(10): 1260-1265.
  • Bamikole MA, Babayemi OJ. 2004. Feeding goats with Guinea grass, veruno stylo and nitrogen fertilized grass with energy concentrate, Archi Zoot, 53: 13-23.
  • Barnes RF, Baylor JE. 1995. Forages in a changing world. In: Barnes RF, Miller DA, Nelson CJ. (eds) Forages, Vol. 1. An introduction to grassland agriculture. Iowa State University Press, Ames, Iowa, USA, pp: 3-13.
  • Bawala TO, Isah OA, Akinsoyinu AO. 2006. Studies on milk mineral composition of lactating West African Dwarf goats. J Anim Vet advances 5(10): 805-809.
  • Beauchemin KA, McGinn SM, Benchaar C, Holtshausen L. 2009. Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: effects on methane production, rumen fermentation, and milk production. J Dairy Sci, 92(5): 2118-2127.
  • Boadi D, Benchaar C, Chiquette J, Masse D. 2004. Mitigation strategies to reduce enteric methane emissions from dairy cows: Update review. Canadian J Anim Scie, 84: 319-335.
  • Bonhomme A. 1990. Rumen ciliates: Their metabolism and relationships with bacteria and their hosts. Anim Feed Sci Tech, 30: 203-266.
  • Carulla J, Kreuzer M, Machmueller A, Hess H. 2005. Supplementation of acacia mearnsii tannins decrease methanogenesis and urinary nitrogen in forage-fed sheep. Australian J Agri Research, 56: 5022.
  • Chynoweth DP. 1996. Environmental impact of biomethanogenesis. Envi Moni Asses, 42: 3-18.
  • Cook BG, Pengelly BC, Schultze-Kraft R, Taylor M, Burkart S, Cardoso Arango JA, et al. 2020. Tropical forages: An interactive selection tool, 2nd and revised Edn. Cali; Nairobi: International center for tropical agriculture (CIAT); Colombia and international livestock research institute (ILRI). Available online at: www.tropicalforages.info (accessed date: January 15, 2025).
  • Demeyer D, De Meulemeester M, De Graeve K, Gupta BW. 1988. Effect of fungal treatment on nutritivevalue of straw. Med Fac Landbouww Rijks univ Gent 53: 1811-1819.
  • Dhiman TR, Satter LD. 1997. Yield response of dairy cows fed different proportions of alfafa silage and corn silage. J Dairy Sci, 80: 2069-2082.
  • Fievez V, Babayemi OJ, Demeyer D. 2005. Estimation of direct and indirect gas production in syringes: a tool to estimate short chain fatty acid production requiring minimal laboratory facilities. Anim Feed Sci Tech, 128-124, 197-210.
  • Forage and Grazing Terminology Committee 1991. Terminology for grazing Lands and grazing animals. Pocahontas Press, Blacksburg, Virginia, USA, pp: 45-60.
  • Getachew G, Makkar HPS, Becker K. 1999. Stochiometric relationship between short chain fatty acid and in vitro production in presence and absence of polyethylene glycol for tannin containing browses. In: FAAP satellite symposium gas production. Fermentation Kinetics for feed evaluation and to assess microbial activity. August 18-19 Wageningen, Netherlands, pp: 78.
  • Houghton J. 1997. Global warming. Geol Magaz, 135(6): 819-842.
  • Intergovernmental Panel on Climate Change (IPCC). 2013. The physical science basis. In Working group i contribution to the fifth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge, UK, pp: 54.
  • Janssen PH. 2010. Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics, Anim Feed Scie Tech, 160(12): 1-22.
  • Johnson KA, Johnson DE. 1995. Methane emissions from cattle. J Anim Sci, 73: 2483-2492.
  • Jouany JP. 1994. Les fermentations dans le rumen et leur optimisation. INRA Prod Anim 7(3): 207-225.
  • Kearny CC. 2005. Effects of dietary physical form and carbohydrate profile on captive giraffe. MSc Thesis, University of Florida, Gainesville, Florida, USA, pp: 87.
  • Leng RA. 1993. Quantitative ruminant nutrition-a green science. Australian J Agri Research, 44(3): 363-380.
  • Man NV, Wiktorsson H. 2003. Forage yield, nutritive value, feed intake, and digestibility of three grass species as affected by harvest frequency. Trop Grassland, 37: 101-110.
  • Mbah CN, Mbah EC, Orji JE, Igberi C, Abam P, Awere SU. 2024. Using Gliricidia sepium prunings as green manure in a degraded ultisol; effects on soil physical properties and yield of okra (Abelmuschus esculentus) in Abakaliki, southeast Nigeria. Biol Agri Hort, 40(4): 257-266.
  • McAllister TA, Cheng KJ, Okine EK, Mathison GW. 1996. Dietary, environmental, and microbiological aspects of methane production in ruminants. Canadian J Anim Sci 76(2): 231-243.
  • Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor. J Agri Sci, 93: 217.
  • Menke KH. Steingass. 1988. Estimation of the energy of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev, 28: 1-7.
  • Merry RJ, Lee MRF, Davies DR, Dewhurst RJ, Moorby JM, Scollan ND, Theodorou MK. 2006. Effects of high- sugar ryegrass silage and mixtures with red clover silage on ruminant digestion. I. In vitro and in vivo studies of nitrogen utilization. J Anim Sci, 84: 3049-3060.
  • Merten DR. 1997. Creating a system for meeting the fibre requirement of Dairy cattle. J Dairy Sci, 80: 1463-1482.
  • Moe PW, Tyrrell HF. 1979. Methane production in dairy cows. J Dairy Sci, 62(10): 1583-1586.
  • Norton BW. 2010. The Nutritive value of tree legumes, http://www.fao.org/ag/AGP/doc/Publication/Guttshel/×5556e0.html (accessed date: January 15, 2025).
  • Oba M, Allen MS. 1999. Evaluation of the importance of the digestibility of neutral detergent fibre from forage: Effects on dry matter intake and milk yield of dairy cows. J Dairy Sci, 82: 529-596.
  • Odeyinka SM. 2001. Effect of feeding varying levels of Leucaena leucocephala and Gliricidia sepium on the performance of West African Dwarf goats. Nigerian. J Anim Prod, 28(1): 61-64.
  • Orskov ER, McDonald I. 1979. The estimation of protein degradability in rumen from incubation measurements weighted according to the rate of passage. J Agri Sci Cambridge, 92: 449-503.
  • Preston TR. 1995. Tropical animal feeding. A manual for research workers. FAO Anim Prod Heal Paper 126, pp: 305.
  • Qin WZ, Li CY, Kim JK, Ju JG, Song MK. 2012. Effects of defaunation on fermentation characteristics and methane production by rumen microbes in vitro when incubated with starchy feed sources. Asian-Australasian J Anim Scie, 25(10): 1381-8. http://doi:10.5713/ajas.2012.12240.
  • Righi F, Ruini P, Romanelli S, Renzi M, Rossi F, Quarantelli A. 2008. Study of alternative field systems for the evaluation of total mixed ration physical form. Corso di Vet med - Università degli Studi di Parma Vol. 28: pp: 181- 190.
  • Shibata M. 1992. Methane production in heifers, sheep and goats consuming diets of various hay-concentrate ratios. J Anim Sci Tech, 63: 1221-1227.
  • Statistical Analysis System Institute Inc 1990 SAS/STAT® user’s guide Int Volume 1, version 6, Fourth Edition, Cary, NC, USA.
  • Swan CG, Bowman JGP, Martin JM, Giroux MJ. 2006. Increased puroindoline levels slow ruminal digestion of wheat (Triticum aestivum L.) starch by cattle. J Anim Sci Tech. 84: 641-650.
  • Theodorou MK, Williams BA, Dhanoa MS, McAllen AB, France J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci Tech, 48: 185-197.
  • Van Soest PJ, Robertson JB, Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci, 74(10): 3583-97.
  • Zhang J, Shi H, Wang Y, Li S, Cao Z, Ji S, He Y, Zhang H. 2017. Effect of dietary forage to concentrate ratios on dynamic profile changes and interactions of ruminal microbiota and metabolites in holstein heifers. Fronti in Microb. 8: 2206.
Toplam 50 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Hayvansal Üretim (Diğer)
Bölüm Research Articles
Yazarlar

Godswill Arinzechukwu Iwuchukwu 0009-0001-3621-7055

Olatunbosun Odu 0009-0002-1274-6902

Uğur Şen

Yayımlanma Tarihi 15 Mayıs 2025
Gönderilme Tarihi 3 Kasım 2024
Kabul Tarihi 14 Ocak 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 8 Sayı: 3

Kaynak Göster

APA Iwuchukwu, G. A., Odu, O., & Şen, U. (2025). Evaluation of Associative Effects of Gliricidia sepium and Megathyrsus maximus Combinations on Fibre Fractions, In-Vitro Carbon Dioxide and Methane Production. Black Sea Journal of Agriculture, 8(3), 295-303. https://doi.org/10.47115/bsagriculture.1564072

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