Chromosome Banding Properties of Neogobius fluviatilis (Pallas, 1814) (Perciformes, Gobiidae)

Yıl 2023, , 600 - 603, 15.10.2023

Sevgi Ünal Karakuş , Muradiye Karasu Ayata , Muhammet Gaffaroğlu

https://doi.org/10.34248/bsengineering.1355295

Öz

The monkey goby, Neogobius fluviatilis (Pallas, 1814) that distributed in Türkiye was studied cytogenetically for the first time. In this context, diploid chromosome number, chromosome morphology and also chromosomal banding properties (C-banding and Ag-NOR staining) of N. fluviatilis were revealed out. Chromosome slides were prepared from head kidney cells according to the air-drying technique. Chromosome slides were observed under the microscope and metaphases were photographed. The chromosomes were measured by digital caliper and karyotype was arranged manually. The diploid chromosome number was found as 46. Karyotype was composed with all uniarmed chromosomes. Fundamental arm number was calculated as 46 too. No heteromorphic sex chromosomes were determined in the karyotype. C-bands were detected on the pericentromeres of almost all chromosomes. Otherwise, two Ag-NORs were found in the silver-stained metaphases. This study revealed out chromosomal properties of N. fluviatilis from Türkiye with conventional cytogenetic techniques. This report may improve the cytogenetic data of the genus Neogobius.

Anahtar Kelimeler

Karyotype, C-banding, Nucleolus Organizer Region, Monkey Goby

Etik Beyan

The experimental procedures were approved by the Local Animal Care and Ethics Committee of Kırşehir Ahi Evran University (Approve number: 68429034/09).

Kaynakça

• Arai R. 2011. Fish karyotypes: A check list. Springer, Tokyo, Japan, pp: 340.
• Arslan A, Arslan E. 2007. Importance of C-banding (Constitutive Heterochromatin) in karyosystematic. Selçuk Univ J Sci Fac, 2(29): 121-126.
• Bertollo LAC, Cioffi MB, Moreira-Filho O. 2015. Direct chromosome preparation from freshwater teleost fishes. In: Ozouf-Costaz C, Pisano E, Foresti F, Toledo LFA, editors. Fish cytogenetic techniques, ray-fin fishes and chondrichthyans. CRC Press, New York, US, pp: 21-26.
• Bigaliev AB, Kobegenova SS, Adyrbekova KB, Gutsulyak SA. 2017. Diversity, morphology and karyotypes of species from genus Neogobius (Perciformes; Gobiidae) at coastal zone of the Caspian Sea (Aktau city). Int J Biol Chem, 10(1): 4-8.
• Caputo V. 1998. Nucleolar organizer (NOR) location and cytotaxonomic implications in six species of gobiid fishes (Perciformes, Gobiidae). Ital J Zool, 65(1): 93-99.
• Çiçek E, Sungur S, Fricke R. 2020. Freshwater lampreys and fishes of Turkey; a revised and updated annotated checklist 2020. Zootaxa, 4809(2): 241-270.
• Ene AC. 2003. Chromosomal polymorphism in the goby Neogobius eurycephalus (Perciformes: Gobiidae). Mar Biol, 142: 583-588.
• Ergene-Gözükara S, Çavaş T. 2002. Cytogenetic analysis of a Mediterranean gobiid fish Gobius paganellus L., 1758 from Turkey. Folia Biol, 50(1-2): 5-7.
• Esmaily AH, Kalbassi MR. 2008. Karyological study on bighead goby (Neogobius kessleri) from southern part of the Caspian Sea. Iran J Fish Sci, 7(2): 15-26.
• Howell WM, Black DA. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36: 1014-1015.
• Karakuş U, Top N, Tepeköy EG, Britton JR, Tarkan AS. 2018. Life history characteristics of the potentially invasive Ponto-Caspian goby Neogobius fluviatilis in natural lakes from its native range (Black Sea region of Turkey). Mar Freshw Res, 69: 1544-1556.
• Levan A, Fredga K, Sandberg AA. 1964. Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201-220.
• Mandrioli M, Manicardi GC, Machella N, Caputo V. 2001. Molecular and cytogenetic analysis of the goby Gobius niger (Teleostei, Gobiidae). Genetica, 110: 73-78.
• Ocalewicz K, Sapota M. 2011. Cytogenetic characteristics of the round goby Neogobius melanostomus (Pallas, 1814) (Teleostei: Gobiidae: Benthophilinae). Mar Biol Res, 7(2): 195-201.
• Özuluğ M, Gaygusuz Ö, Gaygusuz ÇG, Kaya N, Saç G. 2023. Fishes encountered in the Turkish Thrace River systems (NW Turkey). Inland Water Biol, 16(2): 341-356.
• Sumner AT. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res, 75: 304-306.
• Thode G, Alvares MC. 1983. The chromosome complements of two species of Gobius (Teleostei, Perciformes). Experientia, 39: 1312-1314.
• Unal-Karakus S, Gaffaroğlu M, Karasu-Ayata M. 2023. Chromosomal analysis of Sander lucioperca (L., 1758) (Perciformes: Percidae) from Turkey. Menba J Fisher Fac, 9(1): 1-6.