Araştırma Makalesi

False Positives in Luminal Testing

Cilt: 7 Sayı: 1 15 Ocak 2024
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False Positives in Luminal Testing

Öz

The blood sample is one of the most essential pieces of evidence that helps criminal experts in the elucidation of the crime. However, cleaning the blood found at the crime scene after the crime is committed makes it difficult to detect the crime. Therefore, experts have attached great importance to research on blood samples left at the crime scene. Although many test kits are used, especially in detecting erased blood, the luminol kit is widely used at crime scenes. In the luminol kit, the reaction takes place with the addition of hydrogen peroxide (H2O2). Since it is known that hydrogen peroxide can react with other substances containing Fe (II) ions that may be present in the environment during the reaction, it was aimed to carry out research to determine which substances luminol interferes with, especially those that may be found in a domestic crime scene in this study. In domestic crime scenes, there are limited substances that can replicate the distinct, enduring luminescence characteristic of a reaction between luminol and genuine bloodstains, while the uncharacteristic glow produced from many other surfaces is likely to be recognised with the naked eye by a good expert, experienced and knowledgeable in the field. In this regard, the prepared samples were treated with luminol in a dark environment, and the reactions occurring in the first seconds were recorded. The results obtained with this reagent are recommended to be supported by other blood test reagents or confirmatory tests, as there are substances that give false positive results with luminol reagents in the present study.

Anahtar Kelimeler

Destekleyen Kurum

Bu çalışma sırasında, yapılan araştırma konusu ile ilgili doğrudan bağlantısı bulunan herhangi bir kurum veya firmadan, çalışmanın değerlendirme sürecinde, çalışma ile ilgili verilecek kararı olumsuz etkileyebilecek maddi ve/veya manevi herhangi bir destek alınmamıştır.

Etik Beyan

Yazarlar makalenin etik kurul izni gerektirmeyen araştırmalardan biri olduğunu beyan etmiştir.

Teşekkür

Bu çalışmanın yapılmasında; laboratuvar olanak ve elverişli çalışma ortamı sunan, İstanbul Üniversitesi-Cerrahpaşa, Adli Tıp ve Adli Bilimler Enstitüsü Adli Bilimler Laboratuvarı’na ve Kütahya Sağlık Bilimleri Üniversitesi, Uygulama ve Araştırma Merkezi Laboratuvarı’na teşekkürlerimizi sunarız.

Kaynakça

  1. Adair TW, Gabel R, Shimamoto S, Tewes R. 2008. A comparison of the luminol and Blue star blood reagents in detecting blood in soil nearly four years after deposition. IABPA News, 24(4): 5-8.
  2. An JH, Shin KJ, Yang WI, Lee HY. 2012. Body fluid identification in forensics. BMB Rep, 45(10): 545–53. https://doi.org/10.5483/BMBRep.2012.45.10.206.
  3. Arnhold J, Mueller S, Arnold K, Grimm E. 1991. Chemiluminescence intensities and spectra of luminol oxidation by sodium hypochlorite in the presence of hydrogen peroxide. J Biolumin Chemilumin, 6(3): 189-192.
  4. Arnhold J, Mueller S, Arnold K, Sonntag K. 1993. Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite. J Biolumin Chemilumin, 8(6): 307-313. https://doi.org/10.1002/bio.1170080604.
  5. Barni F, Lewis SW, Berti A, Miskelly GM, Lago G. 2007. Forensic application of the luminol reaction as a presumptive test for latent blood detection. Talanta, 72(3): 896–913. https://doi.org/10.1016/j.talanta.2006.12.045.
  6. Castello A, Alvarez M, Verdu F. 2002. Accuracy, reliability, and safety of luminol in bloodstain investigation. Canadian Soc Foren Sci J, 35(3): 113-121.
  7. Cheyne M. 2011. Illuminating latent blood. Application methods, fixatives, alternatives and new formulas for luminol. Corpus, 1-222.
  8. Chourasiya L, Mahakalkar A. 2017. To compare and study differential detection of human and animal blood using phenolphthalein, tetramethylbenzidine and luminol assays. Int J Recent Trends Eng Res, 3(11): 82-102.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Adli Biyoloji

Bölüm

Araştırma Makalesi

Erken Görünüm Tarihi

1 Ocak 2024

Yayımlanma Tarihi

15 Ocak 2024

Gönderilme Tarihi

15 Kasım 2023

Kabul Tarihi

1 Ocak 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 7 Sayı: 1

Kaynak Göster

APA
Gülekçi, Y., & Cavus Yonar, F. (2024). False Positives in Luminal Testing. Black Sea Journal of Engineering and Science, 7(1), 129-138. https://doi.org/10.34248/bsengineering.1391613
AMA
1.Gülekçi Y, Cavus Yonar F. False Positives in Luminal Testing. BSJ Eng. Sci. 2024;7(1):129-138. doi:10.34248/bsengineering.1391613
Chicago
Gülekçi, Yakup, ve Fatma Cavus Yonar. 2024. “False Positives in Luminal Testing”. Black Sea Journal of Engineering and Science 7 (1): 129-38. https://doi.org/10.34248/bsengineering.1391613.
EndNote
Gülekçi Y, Cavus Yonar F (01 Ocak 2024) False Positives in Luminal Testing. Black Sea Journal of Engineering and Science 7 1 129–138.
IEEE
[1]Y. Gülekçi ve F. Cavus Yonar, “False Positives in Luminal Testing”, BSJ Eng. Sci., c. 7, sy 1, ss. 129–138, Oca. 2024, doi: 10.34248/bsengineering.1391613.
ISNAD
Gülekçi, Yakup - Cavus Yonar, Fatma. “False Positives in Luminal Testing”. Black Sea Journal of Engineering and Science 7/1 (01 Ocak 2024): 129-138. https://doi.org/10.34248/bsengineering.1391613.
JAMA
1.Gülekçi Y, Cavus Yonar F. False Positives in Luminal Testing. BSJ Eng. Sci. 2024;7:129–138.
MLA
Gülekçi, Yakup, ve Fatma Cavus Yonar. “False Positives in Luminal Testing”. Black Sea Journal of Engineering and Science, c. 7, sy 1, Ocak 2024, ss. 129-38, doi:10.34248/bsengineering.1391613.
Vancouver
1.Yakup Gülekçi, Fatma Cavus Yonar. False Positives in Luminal Testing. BSJ Eng. Sci. 01 Ocak 2024;7(1):129-38. doi:10.34248/bsengineering.1391613

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