Kemik Kıkırdak Doku Defektlerinde Yeni Tasarlanmış Yapı İskelelerinin Araştırılması

Öz

Kemik dokusu organik ve inorganik bileşenlerden oluşan bir yapıdır. Kemik dokusunun kırılmasıyla ilgili sorunlar mevcuttur. Kırığın kaynaması ve rehabilitasyon süreci çeşitli nedenlerden dolayı uzun zaman almaktadır. Yan etkiler arasında kemik kırıklarının iyileşmesinde gecikme, kemiğin kaynamaması ve enfeksiyonlar yer alır. Son yıllarda yapılan çalışmalar ortopedi cerrahlarını hücresel tedaviye ve biyomateryallere yönlendirmiştir. Çalışmamızda kollajen, lif kabağı, PRP ve kondrosit (kıkırdak hücresi) içeren iskeleleri in vivo iyileşme açısından araştırdık. Çalışma rastgele 4 gruba ayrılan 12 adet tavşan üzerinde gerçekleştirildi. Tavşanlar implantasyondan 8 hafta sonra yüksek doz anestezi uygulanarak sakrifiye edildi. Ameliyatından sonra 8. haftada sakrifiye edilen hayvanlardan alınan numuneler üzerinde histolojik analiz yapıldı. Sonuçlarımız lif kabağı+PRP+kondrosit hücre içeren iskelelerinin biyouyumlu olduğunu ve iyileştirme mühendisliğine mükemmel bir alternatif olduğunu gösterdi.

Anahtar Kelimeler

• Kemik-kıkırdak defektleri
• Yapi iskeleleri
• Lif kabağı

Investigation of Newly Designed Scaffolds in Bone Cartilage Tissue Defects

Öz

Bone tissue is a structure composed of organic and inorganic components. There are problems with the fracture of bone tissue. The fracture healing and rehabilitation are a long term processes for various reasons. Complications of bone fractures include delayed healing, bone nonunion, and infections. Studies conducted in recent years have directed orthopedic surgeons to cellular therapy and biomaterials. Our study investigated loofah, PRP, and chondrocyte (cartilage cell) scaffolds for in vivo healing. The study was carried out on rabbits randomly divided into 4 groups. Animals were sacrificed 8 weeks after implant surgery by applying high-dose anesthesia. Histological analyses were performed on samples from animals sacrificed in week 8 after implant surgery. Our results showed that loofah+PRP+chondrocyte cell scaffolds are biocompatible and an excellent alternative to remedial engineering.

Anahtar Kelimeler

• Bone-cartilage defect
• Scaffolds
• Loofah

Kaynakça

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