Determination of Antimicrobial Activity of Commercially Sold Spirulina Platensis Extracts Obtained With Three Different Solvents against Selected Pathogenic Bacteria and Fungi

Öz

Members of the genus Spirulina, like many other species of cyanobacteria, are known to have the potential to produce numerous antimicrobial substances. In our study, extracts of the commercial powder form of Spirulina platensis were tested using three different solvents (acetone, hexane, methanol) against seven bacterial strains (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) and two fungal species (Candida albicans ATCC 10231, Aspergillus niger ATCC 16404) at five different concentrations (30 mg/ml, 15 mg/ml, 6 mg/ml, 3 mg/ml and 1 mg/ml) using the disc diffusion method. According to our results, it was observed that the extract obtained from methanol gave the best result among the solvents used. The extract obtained from methanol showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 and Pseudomonas aeruginosa ATCC 27853 strains, The extract obtained from hexane showed activity against Bacillus cereus ATCC 10987 and Bacillus subtilis ATCC 6623 strains, while the extract obtained from acetone showed antimicrobial activity only against Pseudomonas aeruginosa ATCC 27853 strain. The extracts obtained from methanol, acetone and hexane did not show activity against the fungi used.

Anahtar Kelimeler

• Spirulina platensis
• Antimicrobial activity
• Extract
• Disc diffusion

Ticari Olarak Satılan Spirulina Platensis’in Üç Farklı Çözücü İle Elde Edilen Ekstraktlarının Seçilmiş Patojenik Bakteri ve Mantarlara Karşı Antimikrobiyal Aktivitelerinin Belirlenmesi

Öz

Spirulina cinsi üyelerinin, diğer birçok siyanobakteri türü gibi çok sayıda antimikrobiyal madde üretme potansiyeline sahip olduğu bilinmektedir. Çalışmamızda, ticari olarak satılan toz formundaki Spirulina platensis’in üç farklı çözücü (aseton, hekzan, metanol) kullanılarak elde edilen ekstraktlarının yedi bakteri suşu (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) ve iki fungus türüne (Candida albicans ATCC 10231, Aspergillus niger ATCC 16404) karşı beş farklı konsantrasyonda (30 mg/ml, 15 mg/ml, 6 mg/ml, 3 mg/ml ve 1 mg/ml) disk difüzyon yöntemi kullanılarak antimikrobiyal aktivitesinin belirlenmesi amaçlanmıştır.Sonuçlarımıza göre, kullanılan çözücüler arasında en iyi sonucu metanolden elde edilen ekstraktın verdiği görülmüştür. Metanolden elde edilen ekstrakt Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 ve Pseudomonas aeruginosa ATCC 27853 suşlarına karşı antimikrobiyal aktivite gösterirken, hekzandan elde edilen ekstrakt Bacillus cereus ATCC 10987 ve Bacillus subtilis ATCC 6623 suşlarına karşı aktivite göstermiş, asetondan elde edilen ekstrakt ise sadece Pseudomonas aeruginosa ATCC 27853 suşuna karşı antimikrobiyal aktivite göstermiştir. Metanol, aseton ve hekzandan elde edilen ekstraktlar kullanılan funguslara karşı aktivite göstermemiştir.

Anahtar Kelimeler

• Spirulina platensis
• Antimikrobiyal aktivite
• Ekstrakt
• Disk difüzyon

Kaynakça

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