Production and Characterization of Sporopollenin Reinforced Alginate-Based Composite Films

Yıl 2025, Cilt: 8 Sayı: 5, 1531 - 1536, 15.09.2025

Bahar Akyüz Yılmaz

https://doi.org/10.34248/bsengineering.1697543

Öz

Sporopollenin is a natural biomaterial that offers great potential for various applications due to its biocompatibility, biodegradability, non-toxicity, durability, and high thermal stability. In this study, sporopollenin was extracted from Pinus nigra pollen and incorporated into alginate-based films to utilize these advantageous properties. Sporopollenin samples were added to 100 mL of 1% alginate solution at different concentrations of 0, 5, 10, and 50 mg. Both sporopollenin-free alginate films and sporopollenin-enriched films were comprehensively characterized using FT-IR, TGA, SEM, and MTT analysis methods. The results revealed that sporopollenin obtained from P. nigra pollen was successfully incorporated into the alginate-based films. Furthermore, an increase in the amount of sporopollenin led to enhanced surface roughness. MTT test results also confirmed that the films were non-toxic. The aim of this work is to investigate the potential of sporopollenin as a functional additive in alginate films and to highlight its importance in developing sustainable and biocompatible biomaterials. These findings suggest that sporopollenin holds promise as an innovative biomaterial for various applications, such as food packaging.

Anahtar Kelimeler

Alginate , Biodegradable , Composite , Cytotoxicity

Etik Beyan

Ethics committee approval was not required for this study because there was no study on animals or humans.

Kaynakça

• Akyuz Yilmaz B, Karaduman T, Cicek M, Akata I, Kaya M. 2021. Production and characterization of nontoxic and biodegradable chitosan–ectomycorrhizal fungi spores blend films. Waste Biomass Valor, 12(11): 5899-5908.
• Alshehri SM, Al-Lohedan HA, Chaudhary AA, Al-Farraj E, Alhokbany N, Issa Z, Ahamad T. 2016. Delivery of ibuprofen by natural macroporous sporopollenin exine capsules extracted from Phoenix dactylifera L. Eur J Pharm Sci, 88: 158-165.
• Chiappe C, Demontis GC, Di Bussolo V, Douton MJR, Rossella F, Pomelli CS, Caporali S. 2017. From pollen grains to functionalized microcapsules: a facile chemical route using ionic liquids. Green Chem, 19(4): 1028-1033.
• Dekamin MG, Karimi Z, Latifidoost Z, Ilkhanizadeh S, Daemi H, Naimi-Jamal MR, Barikani M. 2018. Alginic acid: A mild and renewable bifunctional heterogeneous biopolymeric organocatalyst for efficient and facile synthesis of polyhydroquinolines. Int J Biol Macromol, 108: 1273-1280.
• Dyab AK, Mohamed MA, Meligi NM, Mohamed SK. 2018. Encapsulation of erythromycin and bacitracin antibiotics into natural sporopollenin microcapsules: antibacterial, cytotoxicity, in vitro and in vivo release studies for enhanced bioavailability. RSC Adv, 8(58): 33432-33444.
• Kaya M, Akyuz L, Sargin I, Mujtaba M, Salaberria AM, Labidi J, Ceter T. 2017. Incorporation of sporopollenin enhances acid–base durability, hydrophobicity, and mechanical, antifungal and antioxidant properties of chitosan films. J Ind Eng Chem, 47: 236-245.
• Lingait D, Sethy LK, Kumar A. 2024. Biopolymer sporopollenin reinforced pectin/PVA composite films for sustainable packaging application. Sustain Chem Pharm, 41: 101711.
• Martău GA, Mihai M, Vodnar DC. 2019. The use of chitosan, alginate, and pectin in the biomedical and food sector—biocompatibility, bioadhesiveness, and biodegradability. Polym, 11(11): 1837.
• Martins VF, Pintado ME, Morais RM, Morais AM. 2024. Recent highlights in sustainable bio-based edible films and coatings for fruit and vegetable applications. Foods, 13(2): 318.
• Mohammed ASY, Dyab AK, Taha F, Abd El-Mageed I. 2021. Encapsulation of folic acid (vitamin B9) into sporopollenin microcapsules: Physico-chemical characterisation, in vitro controlled release and photoprotection study. Mater Sci Eng C, 128: 112-271.
• Mujtaba M, Yilmaz BA, Cansaran-Duman D, Akyuz L, Yangın S, Kaya M, Khawar KM. 2022. Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay. Polym Bull, 79(1): 519-540.
• Pathak TS, Kim JS, Lee SJ, Baek DJ, Paeng KJ. 2008. Preparation of alginic acid and metal alginate from algae and their comparative study. J Polym Environ, 16: 198-204.
• Rahman MM, Shahid MA, Hossain MT, Sheikh MS, Rahman MS, Uddin N, Hossain I. 2024. Sources, extractions, and applications of alginate: a review. Discov Appl Sci, 6(8): 443.
• Tallis E. 1950. The structure of alginate fibres. J Text Inst Trans, 41(4): 151-158.
• Tavassoli-Kafrani E, Shekarchizadeh H, Masoudpour-Behabadi M. 2016. Development of edible films and coatings from alginates and carrageenans. Carbohydr Polym, 137: 360-374.
• Tønnesen HH, Karlsen J. 2002. Alginate in drug delivery systems. Drug Dev Ind Pharm, 28(6): 621-630.