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Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu

Yıl 2019, Cilt: 6 Sayı: 4, 209 - 214, 31.12.2019
https://doi.org/10.34087/cbusbed.515563

Öz

Amaç: Menisküs zorlanmalara
karşı emici özelliği ile eklem stabilitesini sağlar. Menisküs yırtığı ayağın
hareketi sırasında ortaya çıkan ciddi bir travmadır.



Gereç ve Yöntem: Menisküs yırtığı olan
28 erkek ve 32 kadın hasta çalışmaya dahil edilmiştir. Hastaların fiziksel
incelemede lokal ağrı hissi, şişme, günlük aktivitelerini yerine getirmede
güçlük,  koşma ve yürüme gibi şikayetleri
vardı. Hastaların manyetik rezonans görüntülemeleri değerlendirildi. Menisküs
ağrısı olan dizlerin yanal suprapatellar poş kısmından enjektör ile 5 cc
sinoviyal sıvıları aspire edildi. Örneklerin rutin histolojik takipleri
yapıldıktan sonra parafine gömüldü ve mikrotom ile yarı-ince kesitleri alındı.
İ
mmunohistokimyasal analiz için matriks metaloprotein (MMP-2) ve nükleer faktör kappa (NF-kβ) primer
antikorları kullanıldı. Kesitlerin şık mikroskobu ile fotoğrafları çekildi ve
değerlendirildi.



Bulgular: Bayan bireylerin
eklem sıvılarında lenfosit ve nötrofil hücrelerinde ve hiperplastik
eritrositler ile fibroblast sayısında artış gözlendi. Erkek hastalarda,
fibroblast hücreleri hiperplastik olarak
izlendi ve plazma hücrelerinin arttığı görüldü. MMP-2
ekspresyonunun
plazma hücrelerinde, fibroblastlarda ve nötrofillerde arttığı ayrıca, NF-kβ
ekspresyonunun lenfosit ve polimorfik çekirdeğe sahip hücrelerde pozitif olduğu
izlendi.



Sonuçlar: Menisküs yırtığı
ve enflamasyonu olan eklemlerde artmış sıvının kıkırdak matriksinde hasara
neden olduğu ve buna bağlı olarak
MMP-2
ve
NF-kβ
gibi sitokinlerin ekspresyonunun arttığı, fibroblast hücreleri gibi sentez
yapan hücrelerin yıkıma uğradığı düşünülmektedir.

Kaynakça

  • Gray, J.C. Neural and vascular anatomy of the menisci of the human knee. Journal of orthopaedic and sports physical therapy, 1999, 29(1),23–30.
  • Kurosawa, H, Fukubayashi, T,Nakajima, H, Load-bearing mode of the knee joint: physical behavior of the knee joint with or without menisci. Clinical Orthopaedics and Related Research, 1980, 283-290.
  • Fox, A.J, Wanivenhaus, F, Burge, A.J, Warren, R.F, Rodeo, S.A, The human meniscus: a review of anatomy, function, injury, and advances in treatment. Clinical Anatomy, 2015, 28, 269–287.
  • Koryem, H.K, Wanas, M, Rizk, M.M.A, Kotb, H.T, Naguib, A.H, Shafei, M.M.A.H.E, Naby, H.M.A.A.E. Evaluation of early changes of cartilage biomarkers following arthroscopic meniscectomy in young egyptian adults. Alexandria Journal of Medicine, 2015,51(3),191–197.
  • McNulty, A.L, Rothfusz, N.E, Leddy, H.A, Guilak, F. Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation. Journal of Orthopaedic Research, 2013, 31(7),1039–1045.
  • Lohmander, L.S, Englund, P.M, Dahl, L.L, The long-term consequence of anterior cruciate ligament and meniscus injuries: osteoarthritis. American Journal of Sports Medicine, 2007, 35, 1756–1769.
  • Lotz , M, MartelPelletier, J, Christiansen, C, Brandi, M.L, Bruyere, O, Chapurlat, R, Collette, J, Cooper, C, Giacovelli, G, Kanis, J.A, Karsdal, M.A, Kraus, V, Lems W.F, Meulenbelt, I, Pelletier, JP, Raynauld, J.P, Reiter-Niesert, S, Rizzoli, R, Sandell, L.J, Van Spil, W.E, Reginster, J.Y. Value of biomarkers in osteoarthritis: current status and perspectives. Annals of the Rheumatic Diseases, 2013,72(11),1756–1763.
  • Buma, P, Ramrattan, N.N, van Tienen, T.G, Veth, R.P.H, Tissue engineering of the meniscus. Biomaterials, 2004, 25, 1523–1532.
  • Balakrishnan, L, Nirujogi, R.S, Ahmad, S, Bhattacharjee, M, Manda, S.S, Renuse, S, Kelkar, D.S, Subbannayya, Y, Raju, R, Goel R, Proteomic analysis of human osteoarthritis synovial fluid. Clinical Proteomics, 2014,11, 6.
  • Baragi, V.M, Becher, G, Bendele, A.M, Biesinger, R, Bluhm, H, Boer, J, Deng, H, A new class of potent matrix metalloproteinase 13 inhibitors for potential treatment of osteoarthritis: Evidence of histologic and clinical efficacy without musculoskeletal toxicity in rat models. Arthritis & Rheumatology, 2009, 60(7), 2008-18.
  • Janusz, M.J, Bendele, A.M, Brown, K.K, Taiwo, Y.O, Hsieh, L, Heitmeyer, S.A, Induction of osteoarthritis in the rat by surgical tear of the meniscus: Inhibition of joint damage by a matrix metalloproteinase inhibitor. Osteoarthritis Cartilage, 2002,10, 785-91.
  • Pozgan, U, Caglic, D, Rozman, B, Nagase, H, Turk, V, Turk, B, Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis, Biological Chemistry, 2010, 391(5), 571-9.
  • Hayden, MS, Ghosh, S. Signaling to NF-kappaB, Genes & Development, 2004, 18, 2195–2224.
  • Mitchell, S, Vargas, J, Hoffmann, A, Signaling via the NFkB system. Wiley Interdisciplinary Reviews Systems Biology and Medicine, 2016, 8, 227–241.
  • Wong, E.T, Tergaonkar, V, Roles of NF-κB in health and disease: mechanisms and therapeutic potential. Clinical Science 2009,116(6), 451-465.
  • Auth PC. Orthopedics. In: Ballweg R, Sullivan E, Brown D, Vetrosky D Physician Assistant A Guide to Clinical Practice, 4th edn. Saunders, New York, 2008, pp 474-491.
  • Peña E, Calvo B., Martínez M.A. , Palanca D., . Doblar M. Finite element analysis of the effect of meniscal tears and meniscectomies on human knee biomechanics. Clinical Biomechanics, 2005,20,498-507.
  • Lingen, M.W, Role of leukocytes and endothelial cells in the development of angiogenesis in inflammation and wound healing. Archives of Pathology & Laboratory Medicine 2001, 125, 67–71.
  • Englund, M. A, Guermazi, S, Lohmander, L, The role of the meniscus in knee osteoarthritis: a cause or consequence? Radiologic Clinics of North America 2009, 47, 703-712.
  • Payette, C. P, Blackburn, B. Lamarche, et al. Sex differences in postprandial plasma tumor necrosis factor-alpha, interleukin-6, and C-reactive protein concentrations. Metabolism, 2009,58,1593-1601.
  • Zhou, X, Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research. Current Opinion in Clinical Nutrition and Metabolic Care, 2010, 13, 541-547.
  • Berenbaum, F, Osteoarthritis as an inflammatory disease. Osteoarthritis and Cartilage, 2013,21,16-21.
  • Loeser, R.F, The effects of aging on the development of osteoarthritis. Hospital for Special Surgery, 2012, 18-19.
  • Stone, A.V, Loeser R.F., Vanderman K.S.,. LongD.L, Clark S.C., Ferguson C.M. Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis pathogenesis. Osteoarthritis and Cartilage, 2014, 264e274.
  • Nagase, H , Woessner, J.F.J, Matrix metalloproteinases, Journal of Biological Chemistry, 1999, 274, 21491-21494.
  • Overall, C.M, Molecular determinants of metalloproteinase substrate specificity: matrix metalloproteinase substrate binding domains, modules, and exosites. Molecular Biotechnology, 2002,22, 51-86.
  • Mott, J.D, Werb, Z, Regulation of matrix biology by matrix metalloproteinases. Current Opinion in Cell Biology, 2004, 16, 558-564.
  • Page-McCaw A, EwaldA.J. , Werb, Z. Matrix metalloproteinases and the regulation of tissue remodelling. Nature Reviews Molecular Cell Biology, 2007,8, 221-233.
  • Liu, B, Goode, A.P, Carter, T.E, Utturkar, G.M, Huebner, J.L, Taylor, D.C, Matrix Metalloproteinase Activity and Prostaglandin E2 are Elevated in the Synovial Fluid of Meniscus Tear Patients, Connective Tissue Research, 2017,58(3-4),305-316.
  • Bonizzi, G, Karin, M, The two NF-kappaB activation pathways and their role in innate and adaptive immunity, Trends in Immunology, 2004, 25(6),280–288.
  • Wang, T, Zhang, X, Li, J, The role of NF-nB in the regulation of cell stress responses. International Immunopharmacology, 2002, 1509–1520.
  • Saito, T, Tanaka, S, Molecular mechanisms underlying osteoarthritis development: Notch and NF-κB. Arthritis Research & Therapy,2017, 19,94.

Matrix Metalloprotein-2 and NF-Kβ Protein Expressions in Synovial Cells of Meniscus Tear Patients

Yıl 2019, Cilt: 6 Sayı: 4, 209 - 214, 31.12.2019
https://doi.org/10.34087/cbusbed.515563

Öz

Aim: Meniscus provides joint stability with its absorbent
properties. Meniscal tear is a serious trauma that occurs during movement of
the foot.

Materials and Methods: The present study included 28 male and 32
female patients with meniscal tears. Physical examination of the patient
revealed local pain, swelling, difficulty in performing daily activities, and
walking. Magnetic resonance imaging of the patients were evaluated. The
synovial fluids of 5 cc were aspirated by the injector from the lateral
suprapatellar pouch portion of the knees with meniscus pain. After routine
histological follow-up of the samples, they were embedded in paraffin and
semi-thin sections were taken with microtome. Matrix metalloprotein (MMP-2) and
nuclear factor kappa (NF-kβ) primary antibodies were used for
immunohistochemical analysis of the samples. Sections were photographed with light
microscope and evaluated.

Results: There was an increase in the number of lymphocytes and
neutrophil cells and hyperplastic erythrocytes and fibroblasts in the joint
fluids of female patients. In male patients, fibroblast cells were observed to
be hyperplastic and plasma cells were increased. MMP-2 expression was increased
in plasma cells, fibroblasts and neutrophils besides, NF-kβ expression was
positive in lymphocytes and cells with polymorphic nuclei.







Conclusion: It is believed that increased fluid in the
joints with meniscal tear and inflammation caused damage in the cartilage
matrix and therefore expressions of cytokines such as MMP-2 and NF-kβ were increased,
and synthesizing cells such as fibroblasts were destroyed

Kaynakça

  • Gray, J.C. Neural and vascular anatomy of the menisci of the human knee. Journal of orthopaedic and sports physical therapy, 1999, 29(1),23–30.
  • Kurosawa, H, Fukubayashi, T,Nakajima, H, Load-bearing mode of the knee joint: physical behavior of the knee joint with or without menisci. Clinical Orthopaedics and Related Research, 1980, 283-290.
  • Fox, A.J, Wanivenhaus, F, Burge, A.J, Warren, R.F, Rodeo, S.A, The human meniscus: a review of anatomy, function, injury, and advances in treatment. Clinical Anatomy, 2015, 28, 269–287.
  • Koryem, H.K, Wanas, M, Rizk, M.M.A, Kotb, H.T, Naguib, A.H, Shafei, M.M.A.H.E, Naby, H.M.A.A.E. Evaluation of early changes of cartilage biomarkers following arthroscopic meniscectomy in young egyptian adults. Alexandria Journal of Medicine, 2015,51(3),191–197.
  • McNulty, A.L, Rothfusz, N.E, Leddy, H.A, Guilak, F. Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation. Journal of Orthopaedic Research, 2013, 31(7),1039–1045.
  • Lohmander, L.S, Englund, P.M, Dahl, L.L, The long-term consequence of anterior cruciate ligament and meniscus injuries: osteoarthritis. American Journal of Sports Medicine, 2007, 35, 1756–1769.
  • Lotz , M, MartelPelletier, J, Christiansen, C, Brandi, M.L, Bruyere, O, Chapurlat, R, Collette, J, Cooper, C, Giacovelli, G, Kanis, J.A, Karsdal, M.A, Kraus, V, Lems W.F, Meulenbelt, I, Pelletier, JP, Raynauld, J.P, Reiter-Niesert, S, Rizzoli, R, Sandell, L.J, Van Spil, W.E, Reginster, J.Y. Value of biomarkers in osteoarthritis: current status and perspectives. Annals of the Rheumatic Diseases, 2013,72(11),1756–1763.
  • Buma, P, Ramrattan, N.N, van Tienen, T.G, Veth, R.P.H, Tissue engineering of the meniscus. Biomaterials, 2004, 25, 1523–1532.
  • Balakrishnan, L, Nirujogi, R.S, Ahmad, S, Bhattacharjee, M, Manda, S.S, Renuse, S, Kelkar, D.S, Subbannayya, Y, Raju, R, Goel R, Proteomic analysis of human osteoarthritis synovial fluid. Clinical Proteomics, 2014,11, 6.
  • Baragi, V.M, Becher, G, Bendele, A.M, Biesinger, R, Bluhm, H, Boer, J, Deng, H, A new class of potent matrix metalloproteinase 13 inhibitors for potential treatment of osteoarthritis: Evidence of histologic and clinical efficacy without musculoskeletal toxicity in rat models. Arthritis & Rheumatology, 2009, 60(7), 2008-18.
  • Janusz, M.J, Bendele, A.M, Brown, K.K, Taiwo, Y.O, Hsieh, L, Heitmeyer, S.A, Induction of osteoarthritis in the rat by surgical tear of the meniscus: Inhibition of joint damage by a matrix metalloproteinase inhibitor. Osteoarthritis Cartilage, 2002,10, 785-91.
  • Pozgan, U, Caglic, D, Rozman, B, Nagase, H, Turk, V, Turk, B, Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis, Biological Chemistry, 2010, 391(5), 571-9.
  • Hayden, MS, Ghosh, S. Signaling to NF-kappaB, Genes & Development, 2004, 18, 2195–2224.
  • Mitchell, S, Vargas, J, Hoffmann, A, Signaling via the NFkB system. Wiley Interdisciplinary Reviews Systems Biology and Medicine, 2016, 8, 227–241.
  • Wong, E.T, Tergaonkar, V, Roles of NF-κB in health and disease: mechanisms and therapeutic potential. Clinical Science 2009,116(6), 451-465.
  • Auth PC. Orthopedics. In: Ballweg R, Sullivan E, Brown D, Vetrosky D Physician Assistant A Guide to Clinical Practice, 4th edn. Saunders, New York, 2008, pp 474-491.
  • Peña E, Calvo B., Martínez M.A. , Palanca D., . Doblar M. Finite element analysis of the effect of meniscal tears and meniscectomies on human knee biomechanics. Clinical Biomechanics, 2005,20,498-507.
  • Lingen, M.W, Role of leukocytes and endothelial cells in the development of angiogenesis in inflammation and wound healing. Archives of Pathology & Laboratory Medicine 2001, 125, 67–71.
  • Englund, M. A, Guermazi, S, Lohmander, L, The role of the meniscus in knee osteoarthritis: a cause or consequence? Radiologic Clinics of North America 2009, 47, 703-712.
  • Payette, C. P, Blackburn, B. Lamarche, et al. Sex differences in postprandial plasma tumor necrosis factor-alpha, interleukin-6, and C-reactive protein concentrations. Metabolism, 2009,58,1593-1601.
  • Zhou, X, Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research. Current Opinion in Clinical Nutrition and Metabolic Care, 2010, 13, 541-547.
  • Berenbaum, F, Osteoarthritis as an inflammatory disease. Osteoarthritis and Cartilage, 2013,21,16-21.
  • Loeser, R.F, The effects of aging on the development of osteoarthritis. Hospital for Special Surgery, 2012, 18-19.
  • Stone, A.V, Loeser R.F., Vanderman K.S.,. LongD.L, Clark S.C., Ferguson C.M. Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis pathogenesis. Osteoarthritis and Cartilage, 2014, 264e274.
  • Nagase, H , Woessner, J.F.J, Matrix metalloproteinases, Journal of Biological Chemistry, 1999, 274, 21491-21494.
  • Overall, C.M, Molecular determinants of metalloproteinase substrate specificity: matrix metalloproteinase substrate binding domains, modules, and exosites. Molecular Biotechnology, 2002,22, 51-86.
  • Mott, J.D, Werb, Z, Regulation of matrix biology by matrix metalloproteinases. Current Opinion in Cell Biology, 2004, 16, 558-564.
  • Page-McCaw A, EwaldA.J. , Werb, Z. Matrix metalloproteinases and the regulation of tissue remodelling. Nature Reviews Molecular Cell Biology, 2007,8, 221-233.
  • Liu, B, Goode, A.P, Carter, T.E, Utturkar, G.M, Huebner, J.L, Taylor, D.C, Matrix Metalloproteinase Activity and Prostaglandin E2 are Elevated in the Synovial Fluid of Meniscus Tear Patients, Connective Tissue Research, 2017,58(3-4),305-316.
  • Bonizzi, G, Karin, M, The two NF-kappaB activation pathways and their role in innate and adaptive immunity, Trends in Immunology, 2004, 25(6),280–288.
  • Wang, T, Zhang, X, Li, J, The role of NF-nB in the regulation of cell stress responses. International Immunopharmacology, 2002, 1509–1520.
  • Saito, T, Tanaka, S, Molecular mechanisms underlying osteoarthritis development: Notch and NF-κB. Arthritis Research & Therapy,2017, 19,94.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Cerrahi
Bölüm Araştırma Makalesi
Yazarlar

Murat Baloğlu 0000-0002-3478-1461

Ebru Gökalp-özkorkmaz

Yayımlanma Tarihi 31 Aralık 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 6 Sayı: 4

Kaynak Göster

APA Baloğlu, M., & Gökalp-özkorkmaz, E. (2019). Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 6(4), 209-214. https://doi.org/10.34087/cbusbed.515563
AMA Baloğlu M, Gökalp-özkorkmaz E. Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu. CBU-SBED. Aralık 2019;6(4):209-214. doi:10.34087/cbusbed.515563
Chicago Baloğlu, Murat, ve Ebru Gökalp-özkorkmaz. “Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 Ve NF-Kβ Protein Ekspresyonu”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6, sy. 4 (Aralık 2019): 209-14. https://doi.org/10.34087/cbusbed.515563.
EndNote Baloğlu M, Gökalp-özkorkmaz E (01 Aralık 2019) Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6 4 209–214.
IEEE M. Baloğlu ve E. Gökalp-özkorkmaz, “Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu”, CBU-SBED, c. 6, sy. 4, ss. 209–214, 2019, doi: 10.34087/cbusbed.515563.
ISNAD Baloğlu, Murat - Gökalp-özkorkmaz, Ebru. “Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 Ve NF-Kβ Protein Ekspresyonu”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6/4 (Aralık 2019), 209-214. https://doi.org/10.34087/cbusbed.515563.
JAMA Baloğlu M, Gökalp-özkorkmaz E. Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu. CBU-SBED. 2019;6:209–214.
MLA Baloğlu, Murat ve Ebru Gökalp-özkorkmaz. “Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 Ve NF-Kβ Protein Ekspresyonu”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, c. 6, sy. 4, 2019, ss. 209-14, doi:10.34087/cbusbed.515563.
Vancouver Baloğlu M, Gökalp-özkorkmaz E. Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu. CBU-SBED. 2019;6(4):209-14.