Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu

Yıl 2019, Cilt: 6 Sayı: 4, 209 - 214, 31.12.2019

Murat Baloğlu , Ebru Gökalp-özkorkmaz

https://doi.org/10.34087/cbusbed.515563

Cited By: 1

Öz

Amaç: Menisküs zorlanmalara
karşı emici özelliği ile eklem stabilitesini sağlar. Menisküs yırtığı ayağın
hareketi sırasında ortaya çıkan ciddi bir travmadır.

Gereç ve Yöntem: Menisküs yırtığı olan
28 erkek ve 32 kadın hasta çalışmaya dahil edilmiştir. Hastaların fiziksel
incelemede lokal ağrı hissi, şişme, günlük aktivitelerini yerine getirmede
güçlük,  koşma ve yürüme gibi şikayetleri
vardı. Hastaların manyetik rezonans görüntülemeleri değerlendirildi. Menisküs
ağrısı olan dizlerin yanal suprapatellar poş kısmından enjektör ile 5 cc
sinoviyal sıvıları aspire edildi. Örneklerin rutin histolojik takipleri
yapıldıktan sonra parafine gömüldü ve mikrotom ile yarı-ince kesitleri alındı.
İmmunohistokimyasal analiz için matriks metaloprotein (MMP-2) ve nükleer faktör kappa (NF-kβ) primer
antikorları kullanıldı. Kesitlerin şık mikroskobu ile fotoğrafları çekildi ve
değerlendirildi.

Bulgular: Bayan bireylerin
eklem sıvılarında lenfosit ve nötrofil hücrelerinde ve hiperplastik
eritrositler ile fibroblast sayısında artış gözlendi. Erkek hastalarda, fibroblast hücreleri hiperplastik olarak
izlendi ve plazma hücrelerinin arttığı görüldü. MMP-2 ekspresyonunun
plazma hücrelerinde, fibroblastlarda ve nötrofillerde arttığı ayrıca, NF-kβ
ekspresyonunun lenfosit ve polimorfik çekirdeğe sahip hücrelerde pozitif olduğu
izlendi.

Sonuçlar: Menisküs yırtığı
ve enflamasyonu olan eklemlerde artmış sıvının kıkırdak matriksinde hasara
neden olduğu ve buna bağlı olarak MMP-2
ve NF-kβ
gibi sitokinlerin ekspresyonunun arttığı, fibroblast hücreleri gibi sentez
yapan hücrelerin yıkıma uğradığı düşünülmektedir.

Anahtar Kelimeler

Menisküs yırtığı, Sinoviyal hücreler, MMP-2, NF-kβ

Matrix Metalloprotein-2 and NF-Kβ Protein Expressions in Synovial Cells of Meniscus Tear Patients

Öz

Aim: Meniscus provides joint stability with its absorbent
properties. Meniscal tear is a serious trauma that occurs during movement of
the foot.

Materials and Methods: The present study included 28 male and 32
female patients with meniscal tears. Physical examination of the patient
revealed local pain, swelling, difficulty in performing daily activities, and
walking. Magnetic resonance imaging of the patients were evaluated. The
synovial fluids of 5 cc were aspirated by the injector from the lateral
suprapatellar pouch portion of the knees with meniscus pain. After routine
histological follow-up of the samples, they were embedded in paraffin and
semi-thin sections were taken with microtome. Matrix metalloprotein (MMP-2) and
nuclear factor kappa (NF-kβ) primary antibodies were used for
immunohistochemical analysis of the samples. Sections were photographed with light
microscope and evaluated.

Results: There was an increase in the number of lymphocytes and
neutrophil cells and hyperplastic erythrocytes and fibroblasts in the joint
fluids of female patients. In male patients, fibroblast cells were observed to
be hyperplastic and plasma cells were increased. MMP-2 expression was increased
in plasma cells, fibroblasts and neutrophils besides, NF-kβ expression was
positive in lymphocytes and cells with polymorphic nuclei.

Conclusion: It is believed that increased fluid in the
joints with meniscal tear and inflammation caused damage in the cartilage
matrix and therefore expressions of cytokines such as MMP-2 and NF-kβ were increased,
and synthesizing cells such as fibroblasts were destroyed

Anahtar Kelimeler

Meniscus tear, Sinovial cells, MMP-2, NF-kβ, immunohistochemistry

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