THE INVESTIGATION OF IN VITRO CALLUS FORMATION, REGENERATION AND MICROPROPAGATION OF DEVEGULU HOLLYHOCK, A MEDICINAL PLANT

Öz

Devegülü hollyhock is used by public for the treatment of certain diseases. This study investigated the appropriate protocols for in vitro callus formation, regeneration and micropropagation of various explants of 14-day and 28-day old Devegülü seedlings germinated from the seeds in a sterile environment. Different concentrations of 2,4- dichlorophenoxy acetic acid (2,4-D) were used as plant growth regulator and Murashige and Skoog basal medium was used as basic medium. Regenerated plantlets were firstly transferred to rooting medium to promote root formation, after that they were planted into soil. Both 14-day and 28-day explants produced a higher rate of callus in MS medium including 2,4-D auxin hormone. The regenerated plantlets were obtained from the shoot tip and node explants of 28 days old seedlings after incubation in MS medium for 6 weeks. Direct regeneration from the node and shoot tip was observed in this hormone free medium. Micropropagation rates were 44.4 % and 12.5 % for node and shoot tip explants, respectively. With this study, callus formation, regeneration and micropropagation of the shoot tip and nodes was successfully carried for the first time.

Anahtar Kelimeler

• Devegülü
• In Vitro Regeneration
• Callus
• Hollyhock

Kaynakça

1. Azab A. (2017). Alcea: Traditional Medicine, Current Research and Future Opportunities. Eur Chem Bull, 5: 505-14.
2. Battal A., Görmez G., Türker M. (2019). Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 8(3): 843-851.
3. Bouayed J., Piri K., Rammal H., Dicko A., Desor F., Younos C., Soulimani R. (2007). Comparative Evaluation of the Antioxidant Potential of Some Iranian Medicinal Plants. Food Chemistry, 104(1): 364-368.)
4. Chenar H.M., Kahrizi D., Zebarjadi A. (2015). Effect of Plant Growth Regulators and Explant Type Upon Cell Dedifferentiation and Callus Induction in Chickpea (Cicer arietinum L.). Journal of Applied Biotechnology Reports, 2: 241-4.
5. Cowan M.M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews, 12(4): 564-582.
6. Darvishi E., Kazemi E., Kahrizi D., Chaghakaboudi S.R., Khani Y.(2014). Optimization of Callus Induction in Pennyroyal (Mentha Pulegium). Journal of Applied Biotechnology Reports, 1: 97-100.
7. Dhar U., Joshi M. (2005). Efficient Plant Regeneration Protocol through Callus for Saussurea Obvallata (DC.) Edgew.(Asteraceae): Effect of Explant Type, Age and Plant Growth Regulators. Plant Cell Reports, 24 (4): 195-200.
8. Gang Y.Y, Du G.S., Shi D.J., Wang M.Z., Li X.D., Hua Z.L. (2003). Establishment of in vitro Regeneration System of the Atrichum mosses. Acta Botanica Sinica, 45 (12): 1475-1480.

9. Güner A., Akyıldırım B., Alkayış M.F., Çıngay B., Kanoğlu S.S., Özkan A.M., Öztekin M., Tuğ G.N. (2012). Türkçe Bitki Adları. In: Türkiye Bitkileri Listesi (Damarlı Bitkiler). Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (Eds.), Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, 618.
10. Hemphill J.K., Maier C.G.A., Chapman K.D. (1998). Rapid in-vitro Plant Regeneration of Cotton (Gossypium hirsutum L.). Plant Cell Rep., 17: 273-278.
11. Hosseini Z., Ghasempour H.R., Kahrizi D., Akbari L. (2017). In Vitro Callus Induction and Shoot Regeneration in Hollyhocks (Althaea digitata). Biological, Environmental and Agricultural Sciences, 2:34-40.
12. Ishii Y., Takamura T., Goi M., Tanaka M., 2004. Callus Induction and Somatic Embryogenesis of Phalaenopsis. Plant Cell Rep. 17 (6): 446-450.
13. Kozai T, Smith M.L. (1995). Environmental Control in Plant Tissue Culture-General Introduction and Overview. In:Automation and Envir. Cont. in Plant Tiss. Cult. J. Aitken-Christie, T. Kozai and Smith, M. L. (eds), Kluwer Academic Publ. Netherlands, p 301-318.Luo J., Gould J.H. (2000).
14. In Vitro Shoot Tip Grafting Improves Recovery of Cotton Plants from Culture. Plant Cell Tissue Organ Culture, 57: 211-213
15. Mansuroğlu S., Gürel E. (2001). Mikroçoğaltım. In: Bitki Biyoteknolojisi I. Doku Kültürü ve Uygulamaları, Babaoğlu, M., Gürel, E. ve Özcan, S. (edt.). S.Ü. Vakfı Yayınları, Konya 262-281.
16. Mati E., de Boer H. (2011). Ethnobotany and Trade of Medicinal Plants in the Qaysari Market, Kurdish Autonomous Region, Iraq. Journal of Ethnopharmacology, 133(2): 490-510.
17. Michel Z., Hilaire K.T., Mongomaké K., Georges A.N., Justin K.Y. (2008). Effect of Genotype, Explants, Growth Regulators and Sugars on Callus Induction in Cotton (Gossypium hirsutum L.). Aust J Crop Sci, 2 (1): 1-9.
18. Mubashrah M, Athar H, İkram U, Rahmatullah Q, Mehmooda M, Muhammad A, Muhammad K, 2012. Callogenesis Potential of Cotyledonary Explants of Althaea rosea from Pakistan. Pak. J. Bot., 44: 271-275, Special Issue March.
19. Munir M., Athar H., İkram U., Rahmatullah Q., Mehmooda M., Muhammad A., Muhammad K. (2012). Callogenesis Potential of Cotyledonary Explants of Althaea rosea from Pakistan. Pak. J. Bot., 44: 271-275.
20. Murch S.J., KrishnaRaj S., Saxena P.K. (2000). Tryptophan is a Precursor for Melatonin and Serotonin Biosynthesis in in vitro Regenerated St. John's Wort (Hypericum perforatum L. cv. Anthos) plants. Plant Cell Reports 19. (7): 698-704.
21. Özkaynak E., Samancı B. (2005). Mikroçoğaltımda Alıştırma. Selçuk Tarım ve Gıda Bilimleri Dergisi, 19 (36): 28-36.
22. Özyiğit I.I., Kahraman M.V., Ercan O. (2007). Relation between Explant Age, Total Phenols and Regeneration Response in Tissue Cultured Cotton (Gossypium hirsutum L.). African Journal of Biotechnology, 6 (1), 003-008.
23. Özyigit I.I. (2008). Phenolic Changes during in vitro Organogenesis of Cotton (Gossypium hirsutum L.) Shoot Tips. African Journal of Biotechnology, 7 (8): 1145-1150.
24. Pakseresht G., Kahrizi D., Mansouri M., Ghorbani T., Kazemi N. (2016). Study of Callus Induction and Cell Culture to Secondary Metabolite Production in Hyssopus officinalis L. J Rep Pharma Sci, 5:104-111.
25. Qader S., Awad H. (2014). Evaluation of Antioxidant, Antimicrobial and Cytotoxicity of Alcea kurdica Alef. Jordan Journal of Biological Sciences, 147(1572): 1-5.
26. Raoul S.S., Gilbert C., Hamidou S.F., Yannick T., Yao D., Abdourahamane S., Michle B. (2010). Protocol for Callus and Somatic Embryogenesis Initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of Explant Type, Sugar and Plant Growth Regulator. Australian J. Crop Sci., 4(2): 98-106.
27. Samanthi A., Mohd Puad N., Suhaimi S., Kumara M., Nor Aini A.S. (2013). In vitro Shoot Regeneration from Leaf Explants of Kenaf (Hibiscus cannabinus L.). Sains Malaysiana, 42 (10): 1505-1510.
28. Sivanesan I., Jeong B.R. (2007). Direct Shoot Regeneration from Nodal Explants of Sida cordifolia Linn. In Vitro Cellular & Developmental Biology-Plant, 43(5): 436-441.
29. Sun T., Ho C.T. (2005). Antioxidant Activities of Buckwheat Extracts. Food chemistry, 90 (4): 743-749
30. Uzunhisarcıklı M.E., Vural M. (2012). The Taxonomic Revision of Alcea and Althaea (Malvaceae) in Turkey. Turkish Journal of Botany, 36 (6): 603-636.
31. Wang J., Sun Y., Hu J., Cui G. (2004). Factors Affecting the Frequencies of callus Induction and Plantlet Regeneration in Maize Immature Embryo Culture. Acta Agron Sin, 30: 398-402.
32. Zhang B.H. (2000). Regulation of Plant Growth Regulators on Cotton Somatic Embryogenesis and Plant Regeneration. Biochemistry, 39: 1567.
33. Zouine J., El Hadrami I. (2004). Somatic Embryogenesis In Phoenix dactylifera L. Effect of Exogenous Supply of Sucrose on Proteins, Sugars, Phenolics and Peroxidases Activities during the Embryogenic Cell Suspension Culture. Biotechnology, 3 (2): 114-118.
34. Zouzou M., Hilaire K.T., Mongomaké K., Georges A.N., Justin K.Y. (2008). Effect of Genotype, Explants, Growth Regulators and Sugars on Callus Induction in Cotton (Gossypium hirsutum L.). Aust J Crop Sci, 2(1), 1-9.