Investigation of the Potential Antioxidant and Antidiabetic Effects of Cotinus coggygria Scop.

Abstract

ABSTRACT Objective: The aim of this study is to evaluate and compare the total phenolic content, total flavonoid content, antioxidant activities (ABTS•+ and DPPH• scavenging effects), and α-glucosidase inhibition effects of different parts of Cotinus coggygria Scop. (green leaves, red leaves, branches, and the heartwood) collected at different times. Methods: The total phenolic content of the extracts was determined using the method developed by Folin and Denis and modified by Singleton. The total flavonoid content was determined according to the method developed by Lar'kina and colleagues. To test the antioxidant capacities of the extracts, DPPH• and ABTS•+ scavenging capacity assays were conducted; and to test their antidiabetic effects, α-glucosidase enzyme inhibition assay was performed. Results: It was found that the heartwood extract had the highest values in terms of total phenolic content and total flavonoid content. In the antioxidant activity assays, similar to the results of the total phenolic and total flavonoid content determinations, the heartwood extract also showed the highest content. However, it was observed that all other extracts also exhibited high antioxidant activity, especially against the ABTS•+. In the α-glucosidase inhibition assay conducted to examine the antidiabetic effects of the extracts, it was found that, in addition to red and green leaves, other extracts also possessed antidiabetic activity. Conclusion: The antioxidant and antidiabetic effects of extracts obtained from different parts of C. coggygria, a species used in traditional medicine for its various therapeutic effects, have been scientifically evaluated, proven, and compared. Key Words: α-glucosidase inhibition, ABTS•+, Cotinus coggygria Scop., DPPH•, total flavonoid content, total phenolic content

Keywords

• α-glucosidase inhibition
• ABTS•+
• Cotinus coggygria Scop.
• DPPH•
• total flavonoid content
• total phenolic content

Cotinus coggygria Scop.'un Potansiyel Antioksidan ve Antidiyabetik Etkilerinin İncelenmesi

Öz

ÖZ Amaç: Cotinus coggygria Scop. türünün farklı zamanlarda toplanan farklı kısımlarının (yeşil renkli yapraklar, kırmızı renkli yapraklar, ince dallar ve gövde iç kısmı) toplam fenolik madde, toplam flavonoit madde, antioksidan (ABTS•+ ve DPPH• süpürücü etki) ve α-glukozidaz inhibisyon etkileri açısından değerlendirilmesi ve karşılaştırılması bu çalışmanın amacını oluşturmaktadır. Yöntemler: Ekstrelerin toplam fenolik madde miktarı Folin ve Denis tarafından geliştirilen, Singleton tarafından modifiye edilen yöntem kullanılarak tayin edilmiştir. Toplam flavonoit madde miktarı ise Lar’kina ve arkadaşları tarafından geliştirilen yönteme göre tayin edilmiştir. Ekstrelerin antioksidan kapasitelerinin test edilmesi amacıyla DPPH• ve ABTS•+ süpürücü kapasite tayini deneyleri; antidiyabetik etkilerinin test edilmesi amacıyla α-glukozidaz enzim inhibisyonu deneyleri yapılmıştır. Bulgular: Toplam fenolik madde, toplam flavonoit madde bakımından en yüksek değerlere gövde ekstresinin sahip olduğu bulunmuştur. Antioksidan aktivite deneylerinde de toplam fenolik madde ve toplam flavonoit madde miktar tayini deneylerinin sonuçlarıyla benzer şekilde en yüksek etkiye gövde ekstresinin sahip olduğu bulunmuştur. Bununla beraber diğer bütün ekstrelerin de özellikle ABTS•+’ye karşı yüksek antioksidan etki gösterdiği gözlenmiştir. Ekstrelerin antidiyabetik etkilerinin incelenmesi amacıyla yapılan α-glukozidaz inhibisyonu deneylerinde ise başta kırmızı ve yeşil yaprak olmak üzere diğer ekstrelerin de antidiyabetik etkiye sahip olduğu bulunmuştur. Sonuç: Halk arasında çeşitli tıbbi etkilerinden dolayı kullanılan C. coggygria türünün farklı kısımlarından elde edilen ekstrelerin antioksidan ve antidiyabetik etkileri bilimsel olarak değerlendirilmiş, kanıtlanmış ve karşılaştırılmıştır.

Anahtar Kelimeler

• α-glukozidaz inhibisyonu
• ABTS•+
• Cotinus coggygria Scop.
• DPPH•
• toplam fenolik içerik
• toplam flavonoit içerik

Kaynakça

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