Investigation of Anxiolytic Effect of Cashew (Anacardium Occidentale) Plant Extracts on Brain Tissues with Bioinformatics-Based Lipidome Analysis: Zebrafish Modeling

Öz

Aim: In this study, the mechanism of the anxiolytic effect of the methanol extract of the cashew plant on anxiety induced by predator stress in zebrafish will be investigated at the molecular level in brain tissue. In this study, it is aimed to show the anxiolytic effects of cashew methanol extract in a zebrafish model and to explain these effects at the molecular level by lipidomics analyses. Material and Method: 18 fish exposed to predator stress were divided into 3 groups for use in the experiment. Each of these groups was given the pharmaceutical formulation (diazepam), 100 grams of powdered cashew extract prepared by maceration method in 1 liter of pure methanol and finally normal feed was given to the control group. Results: The experimental group treated with cashew extract and the control group treated with diazepam were successfully separated from each other as a result of the experiments performed. The accuracy, sensitivity and specificity of the discrimination analyses performed were proven by other experiments. In the experimental group, the metabolite profile in the brain tissue of zebrafish in the anxiety state showed significant changes. In the study, 64 metabolites showing significant differences were identified. Conclusion: Cashew (Anacardium occidentale) plant was used as the experimental group and diazepam was used as the control group in the zebrafish model, and its anxiolytic effect was proven thanks to the anacardic acid and tryptophan compounds contained in cashew.

Anahtar Kelimeler

• Anacardium occidentale
• Anxiety
• Anacardic acid
• Lipidomix
• TOF MS/MS
• Zebrafish

Kaju (Anacardium Occidentale) Bitki Ekstrelerinin Anksiyolitik Etkisinin Biyoinformatik Temelli Geliştirilen Lipidom Analiz ile Beyin Dokularında Araştırılması: Zebra Balığı Modellemesi

Öz

Amaç: Çalışmada predatör stresi ile zebra balıklarında oluşturulan anksiyete üzerine kaju bitkisinin metanol ekstraktının anksiyolitik etkisinin mekanizması, beyin dokusunda moleküler düzeyde incelenecektir. Bu çalışmada, kaju metanol ekstraktının zebra balığı modelinde anksiyolitik etkilerini göstermek ve bu etkileri lipidomiks analizleri aracılığıyla moleküler düzeyde açıklamak amaçlanmaktadır. Materyal ve Metot: Deneyde kullanılmak üzere predatör strese maruz bırakılan 18 balığı 3 gruba ayrılmıştır. Bu grupların her birine 3 hafta boyunca farmasötik formülasyon (diazepam), 100 gram toz haline getirilmiş kajunun 1 litre saf metanolde maserasyon yöntemiyle hazırlanmış ekstratı ve son olarak kontrol grubuna normal yem verilmiştir. Bulgular: Kaju ekstresi ile tedavi edilen deney grubu ve diazepam ile tedavi edilen kontrol grubu, gerçekleştirilen deneyler sonucunda başarıyla birbirinden ayrılmıştır. Yapılan ayrım analizlerinin doğruluğu, hassasiyeti ve özgüllüğü başka deneylerle kanıtlanmıştır. Deney grubunda, anksiyete durumundaki zebra balıklarının beyin dokusundaki metabolit profili önemli ölçüde değişiklik göstermiştir. Çalışmada, anlamlı farklılık gösteren 64 metabolit tespit edilmiştir. Sonuç: Zebra balığı modelinde deney grubu olarak kaju (Anacardium occidentale) bitkisi, kontrol grubu olarak diazepam kullanılmış olup kajunun içerdiği anakardik asit ve triptofan bileşikleri sayesinde anksiyolitik etkisi ispatlanmıştır.

Anahtar Kelimeler

• Anacardium occidentale
• Anksiyete
• Anakardik asit
• Lipidomiks
• TOF MS/MS
• Zebra Balığı

Kaynakça

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