The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis

Tuba Ozcan Metin; Nafiye Yılmaz; Banu Yılmaz; Şakir Necat Yılmaz; Mehmet Emin Erdal; İlay Buran; Gülhan Temel; Adem Doğaner

doi:10.17826/cumj.1310778

EN TR

The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis

Abstract

Purpose: The goal of this study was to evaluate the expression of transient receptor potential vanilloid 6 (TRPV6) and plasma membrane Ca2+ ATPase 1 (PMCA1) in the endometrium of infertile women with unexplained infertility (UI) and endometriosis (E), and to compare the findings to healthy fertile women. Materials and Methods: Endometrial expression of TRPV6 and PMCA1 proteins was evaluated during the mid-secretory phase using the immunohistochemical method, and mRNA levels were measured by real-time PCR. Calcium concentrations were determined using Inductively coupled plasma-mass spectrometry (ICP-MS). Results: TRPV6 and PMCA1 protein expression were significantly increased in both luminal and glandular epithelium of UI or E groups compared to the control group. TRPV6 mRNA expression levels were significantly lower in the UI group (0.525 ± 0.087) compared to the control group (0.809 ± 0.073). There was no significant difference in PMCA1 mRNA expression levels between the groups. Calcium concentration was significantly higher in women with UI (median (Q1-Q3): 50.57 [40.67-51.37], compared to the control group (median (Q1-Q3): 24.29 [20.11-25.55]). Conclusion: The present ﬁndings demonstrate altered expressions of TRPV6 and PMCA1 mRNA and protein, along with differences in calcium concentration among patients, especially those with the UI during the window of implantation. These differences might potentially be considered as one of the underlying causes of infertility. Further studies are needed to elucidate the functional roles of these channels and to develop new strategies for pharmaceutical intervention in the treatment of infertility.

Keywords

Calcium metabolism , endometriosis , TRPV6 , PMCA1 , unexplained infertility

Açıklanamayan infertilite ve endometriozisli infertil hastaların mid-sekretuar endometriyumunda TRPV6 ve PMCA1’in ekspresyonu

Öz

Amaç: Bu çalışmanın amacı, açıklanamayan infertilite (Aİ) ve endometriozisi (E) olan infertil kadınların endometriyumunda Transient Reseptör Potansiyel Vanilloid 6 (TRPV6) ve Plazma Membran Ca2+ ATPaz 1 (PMCA1) ekspresyonunu değerlendirmek ve bulguları sağlıklı fertil kadınlarla karşılaştırmaktır. Gereç ve Yöntem: TRPV6 ve PMCA1 proteinlerinin endometriyal ekspresyonu mid-sekretuar faz sırasında, immünohistokimyasal yöntemle değerlendirilmiş ve mRNA seviyeleri real-time PCR ile ölçülmüştür. Kalsiyum konsantrasyonları İndüktif Eşleşmiş Plazma-Kütle Spektrometrisi (ICP-MS) kullanılarak belirlenmiştir. Bulgular: TRPV6 ve PMCA1 protein ekspresyonu kontrol grubu ile karşılaştırıldığında, Aİ ve E gruplarının hem luminal hem de glandular epitelinde anlamlı olarak artış saptanmıştır. TRPV6 mRNA ekspresyon seviyeleri Aİ grubunda (0.525 ± 0.087) kontrol grubuna (0.809 ± 0.073) kıyasla anlamlı derecede düşüktür. PMCA1 mRNA ekspresyon düzeylerinde gruplar arasında anlamlı bir fark yoktur. Kalsiyum konsantrasyonu Aİ’li kadınlarda (medyan (Q1-Q3): 50.57 [40.67-51.37], kontrol grubuna (medyan (Q1-Q3): 24.29 [20.11-25.55]) göre anlamlı derecede yüksek bulunmuştur. Sonuç: Mevcut bulgular, implantasyon penceresi sırasında özellikle Aİ’li hastalarda, kalsiyum konsantrasyonlarındaki farklılıkların yanı sıra, TRPV6 ve PMCA1 mRNA ve proteinlerinin değişen ekspresyonlarını göstermektedir. Bu farklılıklar, potansiyel olarak infertilitenin altında yatan nedenlerden biri olarak düşünülebilir. Bu kanalların fonksiyonel rollerini aydınlatmak ve infertilite tedavisinde farmasötik yaklaşımda yeni stratejiler geliştirmek için daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

Kalsiyum metabolizması , endometriozis , TRPV6 , PMCA1 , açıklanamayan infertilite

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Histoloji ve Embriyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Tuba Ozcan Metin ^*
0000-0003-0624-026X
Türkiye

Nafiye Yılmaz
0000-0002-4041-297X
Türkiye

Banu Yılmaz
0000-0002-7723-9146
Türkiye

Şakir Necat Yılmaz
0000-0003-1759-3052
Türkiye

Mehmet Emin Erdal
0000-0002-6191-2930
Türkiye

İlay Buran
0000-0002-2890-3952
Türkiye

Gülhan Temel
0000-0002-2835-6979
Türkiye

Adem Doğaner
0000-0002-0270-9350
Türkiye

Erken Görünüm Tarihi

25 Eylül 2023

Yayımlanma Tarihi

30 Eylül 2023

Gönderilme Tarihi

9 Haziran 2023

Kabul Tarihi

23 Ağustos 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 48 Sayı: 3

DOI

https://doi.org/10.17826/cumj.1310778

IZ

https://izlik.org/JA44EN33AZ

MLA

Ozcan Metin, Tuba, vd. “The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis”. Cukurova Medical Journal, c. 48, sy 3, Eylül 2023, ss. 815-24, doi:10.17826/cumj.1310778.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

The expression of TRPV6 and PMCA1 in the mid-secretory endometrium of infertile patients with unexplained infertility and endometriosis

Abstract

Keywords

Açıklanamayan infertilite ve endometriozisli infertil hastaların mid-sekretuar endometriyumunda TRPV6 ve PMCA1’in ekspresyonu

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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